Continuous-flow determination of bile acids in serum, and its clinical application.

Mashige, Fumiko; Osuga, Tatsuya; Tanaka, Natsuki; Imai, Katsuyuki; Yamanaka, Masami

doi:10.1093/clinchem/24.7.1150

Cited by 30 publications

(9 citation statements)

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“… 9 The total bile salt was assayed using the enzymatic 3‐α hydroxysteroid dehydrogenase and diaphorase method. 10 Biliary cholesterol levels were measured by a method incorporating cholesterol oxidase and peroxidase. 11 Phospholipid levels were measured using phospholipase, choline oxidase and a peroxidase‐coupled reaction.…”

Section: Methodsmentioning

confidence: 99%

Human biliary β‐glucuronidase activity before and after relief of bile duct obstruction: Is it the major role in the formation of pigment gallstones?

Chen¹,

Shiesh

Tsao

et al. 2000

J of Gastro and Hepatol

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Section: Methodsmentioning

confidence: 99%

Human biliary β‐glucuronidase activity before and after relief of bile duct obstruction: Is it the major role in the formation of pigment gallstones?

Chen¹,

Shiesh

Tsao

et al. 2000

J of Gastro and Hepatol

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“…Bile acid concentration in endosomes was measured by the enzymatic method of Mashige, Osuga, Tanaka and Yamanaka (1978). Briefly, bile acids are oxidized to 3-oxo bile acids in a reaction catalyzed by 3a-hydroxysteroid dehydrogenase.…”

Section: Measuement Of Bile Acid Concentration In Endosomal Fractionsmentioning

confidence: 99%

Androgenic Modulation of Lipid Metabolism at Subcellular Sites in Cholestatic Rats

Hossain

Hornick²

1994

Horm Metab Res

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The present studies examine the modulation of lipid metabolism at subcellular sites in the liver of control and cholestatic rats by a male sex hormone. Subcutaneous injection of testosterone enanthate (5 mg/kg body weight), increased triacylglycerol lipase (TG-lipase) and neutral cholesterol ester hydrolase (CE-hydrolase) activity in endosomes of normal rats. During induced cholestasis, TG-lipase and CE-hydrolase activity in endosomal fractions were decreased compared to those in endosomes of age-matched sham-operated female controls. Following testosterone administration to cholestatic rats, endosomal lipolytic enzyme activity increased to approximate the levels of normal controls. These observations may provide insight into the alterations in lipid metabolism which accompany cholestatic liver disease.

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“…Serum ALP was estimated by kinetic method (Hausamen et al, 1967). Serum BA and serum bilirubin were measured using enzymatic colorimetric determination (Mashige et al, 1978; Walters and Gerarde, 1970).…”

Section: Methodsmentioning

confidence: 99%

Serum bile acid as a screening tool in workers occupationally exposed to mixtures of organic solvents

et al. 2012

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The aim of the present study was to find out the potential impact of occupational exposure to mixtures of organic solvents on liver function tests. Serum bile acid (BA) was evaluated as a marker of early hepatic derangement and compared to routine liver function tests. The study included two comparable groups: 57 male workers exposed to organic solvents from a paints factory and 59 administrative male controls. Occupational and medical data and serum analysis for liver function parameters (BAs, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), total and direct bilirubin) were obtained. Bile acids showed significantly elevated mean levels in the exposed workers compared to the controls (50.2 ± 47.9 and 5.4 ± 2.7 µmol/L, respectively, p < 0.01). Bile acids were elevated in almost all exposed workers (87.7%) but in none of the controls. Bile acids had significant positive correlation with duration of exposure to organic solvents and lifetime hydrocarbon exposure score (HES) (p < 0.01 and p < 0.05, respectively). All other liver function tests exhibited normal mean levels and did not show statistically significant differences between both groups. The performance of BA versus combined liver enzymes is evaluated using receiver-operating characteristic (ROC) curve and likelihood ratios. The area under the curve was significantly high (0.687 > 0.5, p = 0.012) and had optimal decision level (8 µmol/L). Serum BA can be recommended as a screening test for exposure to organic solvents. It can be suggested for follow-up every 1-2 years or accordingly.

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Continuous-flow determination of bile acids in serum, and its clinical application.

Cited by 30 publications

References 0 publications

Human biliary β‐glucuronidase activity before and after relief of bile duct obstruction: Is it the major role in the formation of pigment gallstones?

Human biliary β‐glucuronidase activity before and after relief of bile duct obstruction: Is it the major role in the formation of pigment gallstones?

Androgenic Modulation of Lipid Metabolism at Subcellular Sites in Cholestatic Rats

Serum bile acid as a screening tool in workers occupationally exposed to mixtures of organic solvents

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