Continuous ethanol production from raw starch using a reversibly soluble-autoprecipitating amylase and flocculating yeast cells

Hoshino, Kazuhiro; Taniguchi, Masayuki; Marumoto, Hideji; Fujii, Michihiro

doi:10.1016/0922-338x(90)90218-l

Cited by 26 publications

(7 citation statements)

References 13 publications

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“…Methods reported for the fermentative production of ethanol from starch include (i) simultaneous saccharification and fermentation with a mixed culture of amylolytic and an ethanol-producing micro- organism (Dostálek and Häggström, 1983;Tanaka, 1986;Abouzied and Reddy, 1987;Han and Steinberg, 1987;Verma et al, 2000), (ii) use of both amylolytic enzymes from bacteria and a fermenting yeast together for the saccharification and fermentation of starch (Laluce and Mattoon, 1984;Hoshino et al, 1989Hoshino et al, , 1990, and (iii) addition of glucoamylase to the fermentation broth prior fermentation, which is a common practice in industry (Yamade and Fukushima, 1989). There is little information about fermentation using raw sweet potato starch with mixed cultures of microorganisms.…”

Section: Filter Paper Chromatography Of Supernatant From Mixedculturementioning

confidence: 99%

Ethanol production by mixed-cultures ofPaenibacillus sp. andZymomonas mobilis using the raw starchy material from sweet potato

Liu

et al. 2009

Ann. Microbiol.

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The conversion of raw starchy materials from sweet potato into ethanol in a mixed-culture of an amylolytic bacterium, Paenibacillus sp. and different strains of Zymomonas mobilis were studied. Raw starchy material from sweet potato was hydrolyzed by Paenibacillus sp. to glucose, which is directly used by Z. mobilis for ethanol production. A mixed-culture of Z. mobilis ATCC 29191 and Paenibacillus sp. 9 yielded the highest ethanol concentration (6.89 g/l) in cultures of small volumes. The effects of medium pH (the best pH value were between 5.0 and 6.0) and the supplementation of the medium with glucose on ethanol production were also studied. In a large-scale fermentation, the final concentration of ethanol was 6.60 g/l, which corresponds to 23.24% of the theoretical yield of the ethanol from 50.0 g/l of the starch from raw sweet potato after a 120 h fermentation period.

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Section: Filter Paper Chromatography Of Supernatant From Mixedculturementioning

confidence: 99%

Ethanol production by mixed-cultures ofPaenibacillus sp. andZymomonas mobilis using the raw starchy material from sweet potato

Liu

et al. 2009

Ann. Microbiol.

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“…Several methods reported for the fermentative production of ethanol from starch include (1) simultaneous saccharification and fermentation with a mixed culture of amylolytic and an ethanol-producing microorganism [19], (2) use of amylolytic enzymes from bacteria and fermenting yeast both together for the saccharification and fermentation of starch [20], (3) addition of glucoamylase to the raw starchy materials prior to fermentation, which is a common practice in industry [21].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from the Raw Corn Starch and Food Waste Employing Simultaneous Saccharification and Fermentation Approach

Prajapati

Trivedi

Patel

2014

Waste Biomass Valor

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Response surface methodology based on the 2 4 factorial central composite design (CCD) was applied to optimize the liquefaction and saccharification process for the enhancement of sugar production from raw corn starch. Optimum level of the pre and post-cooking a-amylase and glucoamylase dose were found to be 3.0 and 1.5 U/ml, respectively, while saccharification temperature for the maximum sugar production was found to be 50°C. The quadratic model predicted that maximum conversion efficiency for the enhanced sugar production from raw corn starch was 93.65 % under the above mentioned optimum level of variables. Additional experiments performed in triplicate with the optimized level of variables showed maximum conversion efficiency of 94.12 %, in close agreement between the predicted and experimental results. The generated sugar was subjected for the bioethanol production in separate fermentation system using yeast while simultaneous saccharification and fermentation system was also evaluated.

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“…Research on bioethanol has centered on the development of advanced technologies that improve process efficiency [1][2][3][4], use inexpensive feedstock materials [5][6][7], and genetically engineered microorganisms to increase the conversion of feedstock into ethanol [8][9][10][11]. In fermentation, the relatively high costs associated with raw materials, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…1 The summary of formulation of balance equation and the adaptation of these formulations to the considered reaction system metabolites. d.f.…”

mentioning

confidence: 99%

Flux analysis of recombinant Saccharomyces cerevisiae YPB-G utilizing starch for optimal ethanol production

Çakır

Arğa

Altintas

et al. 2004

Process Biochemistry

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Continuous ethanol production from raw starch using a reversibly soluble-autoprecipitating amylase and flocculating yeast cells

Cited by 26 publications

References 13 publications

Ethanol production by mixed-cultures ofPaenibacillus sp. andZymomonas mobilis using the raw starchy material from sweet potato

Ethanol production by mixed-cultures ofPaenibacillus sp. andZymomonas mobilis using the raw starchy material from sweet potato

Bioethanol Production from the Raw Corn Starch and Food Waste Employing Simultaneous Saccharification and Fermentation Approach

Flux analysis of recombinant Saccharomyces cerevisiae YPB-G utilizing starch for optimal ethanol production

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