Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain

Domingues, Lucília; Dantas, Maria M.; Lima, Nelson; Teixeira, J. A.

doi:10.1002/(sici)1097-0290(19990920)64:6<692::aid-bit8>3.3.co;2-a

Cited by 28 publications

(50 citation statements)

References 12 publications

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“…1). For this system, the maximum ethanol productivity, 9 g/l h, was slightly lower than that obtained with the recombinant strain S. cerevisiae NCYC869-A3/T1 [10] although higher than the reported for conventional systems.…”

Section: Resultscontrasting

confidence: 62%

“…An airlift bioreactor with a concentric draft tube made of Perspex with a working volume of 6.5 l was used [10]. The regulation system allows for temperature control at 30±1 • C, foam level control by addition of antifoam (Sigma A-5551) and pH control by automatic addition of ammonia with a set-point at pH 4.0 ± 0.1.…”

Section: Continuous-culture Experimentsmentioning

confidence: 99%

“…We have previously described the construction of a flocculent Saccharomyces cerevisiae strain [9] for lactose fermentation and its application in a continuous high-cell-density bioreactor for cheese whey treatment with high ethanol productivity [10,11]. The contamination resistance of this system when operated at sufficiently high dilution rates was also shown [12].…”

Section: Introductionmentioning

confidence: 99%

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Aspergillus niger β-galactosidase production by yeast in a continuous high cell density reactor

Domingues

Lima

Teixeira

2005

Process Biochemistry

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The continuous production of extracellular heterologous ␤-galactosidase by a recombinant flocculating Saccharomyces cerevisiae, expressing the lacA gene (coding for ␤-galactosidase) of Aspergillus niger was investigated. A continuous operation was run in a 6.5 l airlift bioreactor with a concentric draft tube using lactose as substrate. Data on the operation with semi-synthetic medium with 50 and 100 g/l initial lactose concentrations are presented. The best result for ␤-galactosidase productivity-6.2 × 10 5 U/1 h-was obtained for a system operating at 0.24 h −1 dilution rate and for a lactose concentration in the feed of 50 g/l. This value represents a 11-fold increase in ␤-galactosidase productivity when compared to batch culture. Together with extracellular ␤-galactosidase production an ethanol productivity of 9 g/1 h was obtained for the bioreactor fed with 50 g/l initial lactose concentration at 0.45 h −1 dilution rate. In addition to ␤-galactosidase and ethanol production, this system allowed for complete lactose metabolism. The feasibility and advantages of using continuous high-cell-density systems operating with flocculent yeast cells for extracellular protein production is clearly shown.

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Section: Resultscontrasting

confidence: 62%

Section: Continuous-culture Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aspergillus niger β-galactosidase production by yeast in a continuous high cell density reactor

Domingues

Lima

Teixeira

2005

Process Biochemistry

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“…This methodology is used to establish the flocculating ability of cells. And their performance taking into account the work of Domingues et al [16] and Branyik et al [17].…”

Section: Sedimentation Testmentioning

confidence: 99%

“…Under this condition, there is no static repulsion [10]. This methodology was developed taking into account the work of Domingues et al [16], Branyik et al [17], and Bellon-Fontaine et al [18].…”

Section: Microbial Adhesion To Hydrocarbons Test (Math Tests)mentioning

confidence: 99%

Physicochemical Characterization of the Yeast Cells and Lignocellulosic Waste Used in Cell Immobilization for Ethanol Production

María¹,

Mussatto²,

Peñuela³

et al. 2017

Yeast - Industrial Applications

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Ethanol is one of the leading alternative fuels. Efforts have increased the development of technologies for producing ethanol efficiently and economically. The continuous fermentation using yeast cells immobilized in low-cost materials is presented as an excellent alternative. We used four lignocellulosic wastes for the inmobilization process. The materials were characterized physicochemically. The composition was determined by the Van Soest method. Zeta potential was measured to establish the hydrophobic or hydrophilic character of the material surfaces. The contact angles measurements were used to confirm the hydrophobic or hydrophilic character and the free energies interaction was established. Images were obtained by scanning electron microscope, and determination of surface areas and volumes was performed by adsorption and desorption isotherms. It was established that cell surface properties are modified by the immobilization process to which they are subjected. It was evident that cell immobilization depended on the properties of the carrier, as well as cell surface properties. Thus, in order to improve the process of cell immobilization, it is essential to understand the type of carrier-cell interactions that occur during the immobilization process, making necessary the knowledge of the main surface characteristics of both the media and of cells that can affect the process.

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Contamination of a high-cell-density continuous bioreactor

Domingues

Lima

Teixeira

2000

Biotechnol. Bioeng.

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Continuous fermentations were carried out with a recombinant flocculent Saccharomyces cerevisiae strain in an airlift bioreactor. Once operating under steady state at a dilution rate of 0.45 h−1, the bioreactor was contaminated with Escherichia coli cells. The faster growing E. coli strain was washed out of the bioreactor and the recombinant, slower growing flocculating S. cerevisiae strain remained as the only species detected in the bioreactor. Flocculation, besides allowing for the realization of high‐cell‐density systems with corresponding unusual high productivity, may be used as a selective property for controlling some contamination problems associated with prolonged continuous operation. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 584–587, 2000.

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Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain

Cited by 28 publications

References 12 publications

Aspergillus niger β-galactosidase production by yeast in a continuous high cell density reactor

Aspergillus niger β-galactosidase production by yeast in a continuous high cell density reactor

Physicochemical Characterization of the Yeast Cells and Lignocellulosic Waste Used in Cell Immobilization for Ethanol Production

Contamination of a high-cell-density continuous bioreactor

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