Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the glycerol production

Dossat, Valérie; Combes, Didier; Alain, Marty

doi:10.1016/s0141-0229(99)00026-5

Cited by 219 publications

(164 citation statements)

References 15 publications

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“…Glycerol has been reported to decrease enzyme activity (imm.) (Belafibako et al, 2002;Dossat et al, 1999) as well as increase their stability (Watanabe et al, 2006), while no product inhibition due to fatty acid acyl ester has been reported. The glycerol effect is more likely due to mass transfer limitation in imm.…”

Section: The Influence Of Internal and External Mass Transfer Limitatmentioning

confidence: 98%

“…enzymes have been carried out by Dossat et al (1999). The larger the fatty acids (more hydrophobic), the larger the decrease in reaction rate with adsorbed glycerol compared to the situation when no adsorbed glycerol was present.…”

Section: The Influence Of Internal and External Mass Transfer Limitatmentioning

confidence: 99%

“…The larger the fatty acids (more hydrophobic), the larger the decrease in reaction rate with adsorbed glycerol compared to the situation when no adsorbed glycerol was present. This suggests formation of a hydrophilic layer of water and glycerol on the carrier, which decreases reaction rates due to lowered mass transfer of hydrophobic substrates (Dossat et al, 1999).…”

Section: The Influence Of Internal and External Mass Transfer Limitatmentioning

confidence: 99%

“…Possible solutions could be CSTRs, PBRs, fluid beds, expanding bed, recirculation membrane reactors or once through reactors with static mixers. All have been tested in laboratory scale (Dossat et al, 1999;Hsu et al, 2004;Nie et al, 2006;Royon et al, 2007;Shaw et al, 2008;Shibasaki-Kitakawa et al, 2007;Shimada et al, 2002;Dubé et al, 2007;Darnoko and Cheryan, 2000). PBRs have been tested in the laboratory by Shaw et al (2008) for biodiesel production from soybean oil using n-hexane:tert-butanol (9:1, v/v) as cosolvent for methanol.…”

Section: Reactor Configurationsmentioning

confidence: 99%

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A review of the current state of biodiesel production using enzymatic transesterification

Fjerbæk

Christensen

Norddahl

2009

Biotech & Bioengineering

662

459

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Enzymatic biodiesel production has been investigated intensively, but is presently employed industrially only in a 20,000 tons/year pilot plant in China (Du et al. [2008] Appl Microbiol Technol 79(3):331-337). This review presents a critical analysis of the current status of research in this area and accentuates the main obstacles to the widespread use of enzymes for commercial biodiesel transesterification. Improved results for enzymatic catalysis are seen with respect to increased yield, reaction time and stability, but the performance and price of the enzymes need further advances for them to become attractive industrially for biodiesel production. Critical aspects such as mass transfer limitations, use of solvents and water activity are discussed together with process considerations and evaluation of possible reactor configurations, if industrial production with enzymes is to be carried out. Results of published studies on the productivity of enzymes are also presented and compared to the use of chemical catalysts.

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Section: The Influence Of Internal and External Mass Transfer Limitatmentioning

confidence: 98%

Section: The Influence Of Internal and External Mass Transfer Limitatmentioning

confidence: 99%

Section: The Influence Of Internal and External Mass Transfer Limitatmentioning

confidence: 99%

Section: Reactor Configurationsmentioning

confidence: 99%

See 2 more Smart Citations

A review of the current state of biodiesel production using enzymatic transesterification

Fjerbæk

Christensen

Norddahl

2009

Biotech & Bioengineering

662

459

View full text Add to dashboard Cite

show abstract

“…Several short chain alcohols (methanol and ethanol) have been used as acyl acceptors in the transesterification of triacylglycerols (TAG) for biodiesel production (Köse et al, 2002;Shimada et al, 1999Shimada et al, , 2002. However, excess methanol would lead to the inactivation of lipase, and a major by-product of such a process, glycerol, could block the immobilized lipase and lower the enzymatic activity Dossat et al, 1999). Recently, some researchers (Xu et al, 2003, Olivier et al, 2006 utilized methyl acetate 0939Ð5075/2008/0300Ð0297 $ 06.00 " 2008 Verlag der Zeitschrift für Naturforschung, Tübingen · http://www.znaturforsch.com · D as acyl acceptor for biodiesel production to significantly enhance the stability of lipase.…”

Section: Introductionmentioning

confidence: 99%

Lipase-Catalyzed Biodiesel Production with Methyl Acetate as Acyl Acceptor

Huang

Yan

2008

Zeitschrift Für Naturforschung C

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Biodiesel is an alternative diesel fuel made from renewable biological resources. During the process of biodiesel production, lipase-catalyzed transesterification is a crucial step. However, current techniques using methanol as acyl acceptor have lower enzymatic activity; this limits the application of such techniques in large-scale biodiesel production. Furthermore, the lipid feedstock of currently available techniques is limited. In this paper, the technique of lipase-catalyzed transesterification of five different oils for biodiesel production with methyl acetate as acyl acceptor was investigated, and the transesterification reaction conditions were optimized. The operation stability of lipase under the obtained optimal conditions was further examined. The results showed that under optimal transesterification conditions, both plant oils and animal fats led to high yields of methyl ester: cotton-seed oil, 98%; rapeseed oil, 95%; soybean oil, 91%; tea-seed oil, 92%; and lard, 95%. Crude and refined cottonseed oil or lard made no significant difference in yields of methyl ester. No loss of enzymatic activity was detected for lipase after being repeatedly used for 40 cycles (ca. 800 h), which indicates that the operational stability of lipase was fairly good under these conditions. Our results suggest that cotton-seed oil, rape-seed oil and lard might substitute soybean oil as suitable lipid feedstock for biodiesel production. Our results also show that our technique is fit for various lipid feedstocks both from plants and animals, and presents a very promising way for the large-scale biodiesel production.

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Ionic Liquids and Whole‐Cell–Catalyzed Processes

Dennewald

Weuster‐Botz

2012

Ionic Liquids in Biotransformations and Organocatalysis

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Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the glycerol production

Cited by 219 publications

References 15 publications

A review of the current state of biodiesel production using enzymatic transesterification

A review of the current state of biodiesel production using enzymatic transesterification

Lipase-Catalyzed Biodiesel Production with Methyl Acetate as Acyl Acceptor

Ionic Liquids and Whole‐Cell–Catalyzed Processes

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