Continuous electrochemical detection of hydrogen peroxide by Au-Ag bimetallic nanoparticles in microfluidic devices

Ko, Euna; Tran, Van‐Khue; Geng, Yanfang; Chung, Woo Sung; Park, Chan Ho; Kim, Min Ki; Jin, Ga Hyun; Seong, Gi Hun

doi:10.1016/j.jelechem.2017.03.027

Cited by 37 publications

(11 citation statements)

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“…However, a few efforts have been made for the electrochemical detection of ROS in microfluidic systems. Ko et al employed bimetallic nanoparticles to continuously detect hydrogen peroxide in microfluidic devices for up to 100 min [ 31 ]. Jin et al modified a microfluidic chip by integrating a flexible electrochemical sensor for monitoring vascular transduction and evaluated the effect of H 2 O 2 on vascular mechanotransduction [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system

et al. 2021

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Hepatic fibrosis is a foreshadowing of future adverse events like liver cirrhosis, liver failure, and cancer. Hepatic stellate cell activation is the main event of liver fibrosis, which results in excessive extracellular matrix deposition and hepatic parenchyma's disintegration. Several biochemical and molecular assays have been introduced for in vitro study of the hepatic fibrosis progression. However, they do not forecast real-time events happening to the in vitro models. Trans-epithelial electrical resistance (TEER) is used in cell culture science to measure cell monolayer barrier integrity. Herein, we explored TEER measurement's utility for monitoring fibrosis development in a dynamic cell culture microphysiological system. Immortal HepG2 cells and fibroblasts were co-cultured, and transforming growth factor β1 (TGF-β1) was used as a fibrosis stimulus to create a liver fibrosis-on-chip model. A glass chip-based embedded TEER and reactive oxygen species (ROS) sensors were employed to gauge the effect of TGF-β1 within the microphysiological system, which promotes a positive feedback response in fibrosis development. Furthermore, albumin, Urea, CYP450 measurements, and immunofluorescent microscopy were performed to correlate the following data with embedded sensors responses. We found that chip embedded electrochemical sensors could be used as a potential substitute for conventional end-point assays for studying fibrosis in microphysiological systems.

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Section: Introductionmentioning

confidence: 99%

Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system

et al. 2021

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“…As a green chemical product, hydrogen peroxide (H 2 O 2 ) has a wide range of applications in environmental [ 1 ], clinical [ 2 ], pharmaceutical [ 3 ], food industry [ 4 ], textiles industry [ 5 ], and other fields. At the same time, H 2 O 2 is also a byproduct of various enzyme catalyzed reactions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Green Preparation of Ag-Au Bimetallic Nanoparticles Supported on Graphene with Alginate for Non-Enzymatic Hydrogen Peroxide Detection

et al. 2018

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In this work, a facile, environmentally friendly method was demonstrated for the synthesis of Ag-Au bimetallic nanoparticles (Ag-AuNPs) supported on reduced graphene oxide (RGO) with alginate as reductant and stabilizer. The prepared Ag-AuNPs/RGO was characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The results indicated that uniform, spherical Ag-AuNPs was evenly dispersed on graphene surface and the average particle size is about 15 nm. Further, a non-enzymatic sensor was subsequently constructed through the modified electrode with the synthesized Ag-AuNPs/RGO. The sensor showed excellent performance toward H2O2 with a sensitivity of 112.05 μA·cm−2·mM−1, a linear range of 0.1–10 mM, and a low detection limit of 0.57 μM (S/N = 3). Additionally, the sensor displayed high sensitivity, selectivity, and stability for the detection of H2O2. The results demonstrated that Ag-AuNPs/RGO has potential applications as sensing material for quantitative determination of H2O2.

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“…04-0783) (curve b). ,, The XRD patterns of AuNPs-COF 2θ peaks at 19.92, 24.12, 38.17, 44.67, 64.62, and 77.72° correspond to (001), (202), (111), (200), (220), and (311) for AuNPs-COF. − , The obtained peaks at 38.37° (111), 44.67° (200), 64.72° (220), and 77.72° (311) are characteristic peaks of AuNPs and also highly crystalline in behavior (JCPDS No. 04-0784). , Finally, the Au-AgNPs-COF showed notable 2θ peaks at 19.77° (001), 24.07° (202), 38.17° (111), 44.22° (200), 64.22° (220), and 77.47° (311), which are characteristics of the bimetallic nanocomponents (curve c). , In the absence of two different 2θ values in the bimetallic composite, nanoparticles are clearly reveal that the system has an alloy nature. Figure S8 (SI SI.5) describes the detailed thermal properties of the COF and its nanocomposites.…”

Section: Resultsmentioning

confidence: 95%

“…The developed bimetallic alloy nanoelectrocatalyst showed better catalytic performance for the reduction of H 2 O 2 over the reported methods (Table ). ,,− ,,− , Chitosan-GQD/Ag nanocubes were used on the GCE and then applied to monitor the enzyme-free H 2 O 2 . The developed sensor had a linear wide concentration range and acceptable LOD .…”

Section: Resultsmentioning

confidence: 99%

“…In addition, an imbalanced levels of H 2 O 2 are closely associated with age-related disorders such as Alzheimer’s and Parkinson’s diseases. , Furthermore, H 2 O 2 has been strongly linked to cardiovascular disease, atherosclerosis, diabetes, neurodegenerative disorders, and abnormal growth of tumor cells. , A reliable, quantitative H 2 O 2 assay in biological cellular systems is required. Sensors of many types have been produced, but researchers are currently looking for sensitive, selective, nonenzymatic sensor targeting biological samples and live cells. − …”

Section: Introductionmentioning

confidence: 99%

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Gold–Silver Bimetallic Alloy Nanoparticles in a Covalent Organic Framework for Real-Time Monitoring of Hydrogen Peroxide from Live Cells

Arul

Huang

Mani

2022

ACS Appl. Nano Mater.

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An efficient and simple way has been described to prepare gold and silver bimetallic alloy nanoparticles (Au-AgNPs) in an organic framework with a metal-free core. The growth of alloy Au-AgNPs was monitored by UV–visible spectroscopy (UV–vis) and confirmed using various spectral, microscopy, and electrochemical techniques. The field-emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) results revealed that the covalent organic framework (COF) had a uniform flake-like morphology, and the alloy-based Au-AgNPs had a flower-like structure. The results of X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) indicated that Au-AgNPs are metallic in nature and highly crystalline. The surface of a glassy carbon electrode (GCE) was then modified with Au-AgNPs-COF, which was subsequently employed for enzyme-free electrochemical reduction of H2O2. The electrocatalytic cyclic voltammetry performance of the different modified electrodes was in the following order: COF (−14.82 μA) < AgNPs-COF (−26.95 μA) < AuNPs-COF (−31.78 μA) < Au-AgNPs-COF (−46.15 μA). The Au-AgNPs-COF/GCE displayed an excellent electrocatalytic activity toward reduction of H2O2, over a dynamic range of 2.0 nM–1.0 mM with a limit of detection (LOD) of 0.44 nM (S/N = 3). Furthermore, the present sensor showed appreciable selectivity, stability, and reproducibility against the reduction of H2O2. Practicality was demonstrated in fetal bovine serum (FBS), cat blood serum (CBS), and living cells (RAW 264.7).

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Continuous electrochemical detection of hydrogen peroxide by Au-Ag bimetallic nanoparticles in microfluidic devices

Cited by 37 publications

References 37 publications

Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system

Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system

Green Preparation of Ag-Au Bimetallic Nanoparticles Supported on Graphene with Alginate for Non-Enzymatic Hydrogen Peroxide Detection

Gold–Silver Bimetallic Alloy Nanoparticles in a Covalent Organic Framework for Real-Time Monitoring of Hydrogen Peroxide from Live Cells

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