Continuous Dielectrophoretic Size-Based Particle Sorting

Kralj, Jason G.; Lis, Michael T. W.; Schmidt, Martin; Jensen, Klavs F.

doi:10.1021/ac0601314

Cited by 151 publications

(136 citation statements)

References 25 publications

Supporting

Mentioning

130

Contrasting

Order By: Relevance

“…12 and developed by Fiedler et al 108 Particles are guided by the angled electrodes to a small exit gap, at which point particle concentration can be considerably enhanced over the starting concentration introduced into the device. Angled electrodes are employed in an integrated DEP chip design for the continuous filtering and sorting of bioparticles, 109 and also used to great effect in a continuous DEP size-based particle sorter 110 and a multitarget DEP activated cell sorter described by Kim et al 111 A simple arrangement for aligning cells in fluidic channels, consisting of two face-to-face strip electrodes mounted on the top and bottom of a microchannel, has been described by Schnelle et al 112 In this device, particles exhibiting negative DEP are brought by fluid flow to an energized electrode pair, and as a result of experiencing repulsion forces from both electrodes are lifted into the central stream of the fluid flow. This basic concept has been refined by Demierre et al 113 who fabricated a microfluidic device based on an arrangement of lateral metal electrodes and a patterned insulator.…”

Section: Technologymentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

View full text Add to dashboard Cite

A review is presented of the present status of the theory, the developed technology and the current applications of dielectrophoresis ͑DEP͒. Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying DEP to unmet needs in such areas as biosensors, cell therapeutics, drug discovery, medical diagnostics, microfluidics, nanoassembly, and particle filtration. The dipole approximation to describe the DEP force acting on a particle subjected to a nonuniform electric field has evolved to include multipole contributions, the perturbing effects arising from interactions with other cells and boundary surfaces, and the influence of electrical double-layer polarizations that must be considered for nanoparticles. Theoretical modelling of the electric field gradients generated by different electrode designs has also reached an advanced state. Advances in the technology include the development of sophisticated electrode designs, along with the introduction of new materials ͑e.g., silicone polymers, dry film resist͒ and methods for fabricating the electrodes and microfluidics of DEP devices ͑photo and electron beam lithography, laser ablation, thin film techniques, CMOS technology͒. Around three-quarters of the 300 or so scientific publications now being published each year on DEP are directed towards practical applications, and this is matched with an increasing number of patent applications. A summary of the US patents granted since January 2005 is given, along with an outline of the small number of perceived industrial applications ͑e.g., mineral separation, micropolishing, manipulation and dispensing of fluid droplets, manipulation and assembly of micro components͒. The technology has also advanced sufficiently for DEP to be used as a tool to manipulate nanoparticles ͑e.g., carbon nanotubes, nano wires, gold and metal oxide nanoparticles͒ for the fabrication of devices and sensors. Most efforts are now being directed towards biomedical applications, such as the spatial manipulation and selective separation/enrichment of target cells or bacteria, high-throughput molecular screening, biosensors, immunoassays, and the artificial engineering of three-dimensional cell constructs. DEP is able to manipulate and sort cells without the need for biochemical labels or other bioengineered tags, and without contact to any surfaces. This opens up potentially important applications of DEP as a tool to address an unmet need in stem cell research and therapy.

show abstract

Section: Technologymentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

View full text Add to dashboard Cite

show abstract

“…In electrode-based dielectrophoresis (eDEP), pairs of microelectrodes are placed inside a microchannel. High-frequency AC voltages are supplied to achieve strong electric fields/gradients and suppress electrochemical reactions on electrode surfaces [10][11][12][13][14][15][16][17]. In insulator-based dielectrophoresis (iDEP), both DC and AC voltages (of any frequency) can be applied to the remote electrodes positioned in end-channel reservoirs for transporting and manipulating particles.…”

Section: Introductionmentioning

confidence: 99%

Joule heating effects on electroosmotic flow in insulator‐based dielectrophoresis

et al. 2011

View full text Add to dashboard Cite

Joule heating effects on electroosmotic flow in insulator-based dielectrophoresisInsulator-based dielectrophoresis (iDEP) is an emerging technology that has been successfully used to manipulate a variety of particles in microfluidic devices. However, due to the locally amplified electric field around the in-channel insulator, Joule heating often becomes an unavoidable issue that may disturb the electroosmotic flow and affect the particle motion. This work presents the first experimental study of Joule heating effects on electroosmotic flow in a typical iDEP device, e.g. a constriction microchannel, under DC-biased AC voltages. A numerical model is also developed to simulate the observed flow pattern by solving the coupled electric, energy, and fluid equations in a simplified two-dimensional geometry. It is observed that depending on the magnitude of the DC voltage, a pair of counter-rotating fluid circulations can occur at either the downstream end alone or each end of the channel constriction. Moreover, the pair at the downstream end appears larger in size than that at the upstream end due to DC electroosmotic flow. These fluid circulations, which are reasonably simulated by the numerical model, form as a result of the action of the electric field on Joule heatinginduced fluid inhomogeneities in the constriction region.

show abstract

“…The proposed method can determine the levitation height of cells subjected to external forces. Another work by Kralj et al [7] modeled the trajectory of microparticles in a continuous flow microdevice, where separation is achieved based on the size of microparticles. Neculae et al [8] did an analysis to predict microentity trajectory by considering all the forces associated with the particle.…”

Section: Related Work and Backgroundmentioning

confidence: 99%

Design and Verification of a Blood Cell Separation Microfluidic Device

2017

View full text Add to dashboard Cite

Abstract-Blood cell separation microdevices are designed in biomedical engineering for the separation of particular cells from blood, such as cancer cells. The movement of blood microentities, especially abnormal ones, in a continuous flow microfluidic device is controlled by several forces. Therefore, understanding and guiding the movement of these microentities is a challenging problem. These cells are subject to different types of forces that result from natural or external effects. These forces include that due to gravity, virtual mass, buoyancy, dielectrophoresis, and inertia. Therefore, these are to be accounted for in any design or implementation of a system. In this paper we use formal analysis of a separation microdevice to model and verify the microenetit's movement and behavior at high level of abstraction while considering different types of forces. The dynamic behavior of the microentity can be modeled as a Markovian decision process to predict the trajectory of the same. This model can provide probabilistic analysis for the microentity movement in the microdevice under the effect of different types of forces.

show abstract

Continuous Dielectrophoretic Size-Based Particle Sorting

Cited by 151 publications

References 25 publications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Joule heating effects on electroosmotic flow in insulator‐based dielectrophoresis

Design and Verification of a Blood Cell Separation Microfluidic Device

Contact Info

Product

Resources

About