Continuous dielectrophoretic cell separation microfluidic device

Li, Youlan; Dalton, Colin; Crabtree, H. John; Nilsson, Gregory; Kaler, K.V.I.S.

doi:10.1039/b613344d

Cited by 141 publications

(99 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20,[25][26][27][28] The dielectrophoretic force is highly sensitive to the cell size, since the force is to a first approximation proportional to the volume. Indeed, for cells with little shape variation, the size dependence of the dielectrophoretic force can directly be used to sort different division stages because of the steady increase in cell size before division.…”

Section: Discussionmentioning

confidence: 99%

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

et al. 2011

View full text Add to dashboard Cite

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main approach today is the use of metabolic agents that block the cell cycle at a particular phase and accumulate cells at this phase, disturbing the cell physiology. We provide here a non-invasive and label-free continuous cell sorting technique to analyze and synchronize yeast cell division. By balancing opposing dielectrophoretic forces at multiple frequencies, we maximize sensitivity to the characteristic shape and internal structure changes occurring during the yeast cell cycle, allowing us to synchronize the culture in late anaphase.

show abstract

Section: Discussionmentioning

confidence: 99%

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Cell focusing using negativeDEP, controlling the distance the cells are repelled from the electrode surfaces, can also be used instead of trapping to implement cell sorting in the original sample. [58][59][60][61] However, this approach may not provide enough selectivity as all cells would likely be focused to the same stream in such high electrical conductivity media and re-suspension or dilution may still be necessary. In any case, a computational model to obtain the net force field and potential particle trajectories will be crucial to allow for the design of carbonDEP devices with an expected functionality.…”

Section: Discussionmentioning

confidence: 99%

Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis

et al. 2016

View full text Add to dashboard Cite

Here, we report on an enrichment protocol using carbon electrode dielectrophoresis to isolate and purify a targeted cell population from sample volumes up to 4 ml. We aim at trapping, washing, and recovering an enriched cell fraction that will facilitate downstream analysis. We used an increasingly diluted sample of yeast, 10 6 -10 2 cells/ml, to demonstrate the isolation and enrichment of few cells at increasing flow rates. A maximum average enrichment of 154.2 6 23.7 times was achieved when the sample flow rate was 10 ll/min and yeast cells were suspended in low electrically conductive media that maximizes dielectrophoresis trapping. A COMSOL Multiphysics model allowed for the comparison between experimental and simulation results. Discussion is conducted on the discrepancies between such results and how the model can be further improved. Published by AIP Publishing.

show abstract

“…This requires that the channel dimensions be scaled to the size of the particles to be manipulated. Recent work has used DEP forces trap bacteria from a continuous-flow sample stream at 100 ll/h (107), and combined DEP forces with hydrodynamic focusing to fractionate yeast cells (108).…”

Section: Particle Positioningmentioning

confidence: 99%

Microfluidic impedance‐based flow cytometry

Berardino²,

et al. 2010

View full text Add to dashboard Cite

Microfabricated flow cytometers can detect, count, and analyze cells or particles using microfluidics and electronics to give impedance-based characterization. Such systems are being developed to provide simple, low-cost, label-free, and portable solutions for cell analysis. Recent work using microfabricated systems has demonstrated the capability to analyze micro-organisms, erythrocytes, leukocytes, and animal and human cell lines. Multifrequency impedance measurements can give multiparametric, high-content data that can be used to distinguish cell types. New combinations of microfluidic sample handling design and microscale flow phenomena have been used to focus and position cells within the channel for improved sensitivity. Robust designs will enable focusing at high flowrates while reducing requirements for control over multiple sample and sheath flows. Although microfluidic impedance-based flow cytometers have not yet or may never reach the extremely high throughput of conventional flow cytometers, the advantages of portability, simplicity, and ability to analyze single cells in small populations are, nevertheless, where chip-based cytometry can make a large impact. ' 2010International Society for Advancement of Cytometry Key terms microfluidics; impedance characterization; label free; single cell analysis; hydrodynamic focusing; sorting MICROFLUIDIC flow cytometers can bring many advantages to the field of flow cytometry (FCM). Compared to typical flow cytometry channel sizes, the miniaturized dimensions permit microfluidic systems to analyze single cells, to identify cellular variability in gene expression, or drug response within a cell population. Chipbased cytometers can have lower size and costs than conventional benchtop instruments, and may be portable. Today, the developmental aim for microfluidic systems is to reach the same sensitivity and capability for multiparametric analyses as delivered by conventional flow cytometers. Many efforts have been made to improve existing devices and to create new miniaturized high-end instruments. Microfluidic chips can incorporate on-chip cell preparation, cell culture, lysis, and modules for optical, electrophoretic, or genomic analysis (1). They are also suitable for analysis of cells in suspension as well as adherent cells.Miniaturized cytometry devices will have high impact in the development of point-of-care devices in developing countries. Accurate CD4 1 T-cell counts are used to monitor human immunodeficiency virus (HIV)-infected patients, and various thresholds of the number of CD4 1 T lymphocytes per ll of whole blood are used to start antiretroviral therapy (2-5). A simple, single-purpose CD4 cell counting device, which does not require standard laboratory equipment or trained laboratory personnel, could help some of the 33 million HIV-infected people worldwide monitor the stage of infection (6)(7)(8). In this application, increased analysis throughput is a secondary concern compared to increased sensitivity and specificity. Portable, miniatu...

show abstract

Continuous dielectrophoretic cell separation microfluidic device

Cited by 141 publications

References 29 publications

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis

Microfluidic impedance‐based flow cytometry

Contact Info

Product

Resources

About