Eco system is deteriorating day by day due to Industrial effluents. The effluents are containing toxic chemicals which are inturn polluting ground drinking water and creating new diseases. Treatment methods for these Industrial effluents are becoming more costly. The Present study is aimed and focused at decreasing the toxicity of Industrial waters and the target is Reduction, Recycle and Reuse (RRR) using Microbial Fuel Cell. Microbial Fuel Cell experiments were carried out in batch operations for Appughar waste water using Bakers yeast (saccharomyces cerevisiae organism) in order to increase oxygen levels and decrease toxicity. The parameters studied are design of MFC, NaCl concentration, Agar concentration, fructose dosage, pH, COD, BOD, DO, TSS, TDS, Sulphates, Chlorides and Power generation. The results produced have high oxygen levels and change of pH is appreciable from 7.8 to 7.0. COD, BOD and DO have changed from 1050 to 450 ppm, 560 to 220 ppm and 2.5 to 4.2 ppm.