Continuous degradation of phenol by Rhodococcus sp. immobilized on granular activated carbon and in calcium alginate

Pai, Shwu-Ling; Hsu, Yu-Lan; Chong, Nyuk-Min; Sheu, Ching-Sen; Chen, Chei-Hsiang

doi:10.1016/0960-8524(94)00078-f

Cited by 65 publications

(35 citation statements)

References 7 publications

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“…Ca-alginate hydrogel was chosen as a model matrix because many papers describe the entrapment of Rhodococcus cells with alginate and especially when rhodococci degrade organic pollutants (e.g. [Pai et al, 1995], [Mukerjee-Dhar et al, 1998] and [Naito et al, 2001]). Complementary studies were carried out to characterize this new system: (i) NMR study of R. rhodochrous metabolism in such an environment, (ii) adsorption measurements of ABT and FeNTA on alginate beads.…”

Section: Introductionmentioning

confidence: 99%

2-Aminobenzothiazole degradation by free and Ca-alginate immobilized cells of Rhodococcus rhodochrous

et al. 2009

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2-Aminobenzothiazole (ABT) degradation was investigated using free and immobilized systems during photodegradation under solar light in the presence of Fe(III)-nitrilotriacetic acid (FeNTA), biodegradation by Rhodococcus rhodochrous, and during combined conditions. Ca-alginate hydrogel was chosen as a model matrix and some complementary studies were required to characterize this new system. R. rhodochrous metabolism in this type of environment was monitored by NMR spectroscopy. Neither change in intracellular pH values nor in ATP concentrations was observed by in vivo 31 P NMR, showing that no metabolic modification occurred between free and immobilized cells.1 H NMR demonstrated that alginate was not used as carbon source by R. rhodochrous. After establishing the pre-treatment protocol by SPE to eliminate solubilised alginate, ABT adsorption on beads and degradation were studied. The same pathways of transformation were observed in suspended and immobilized cell systems. Considering the ABT adsorption phenomenon on alginate beads (8%), the efficiency of the two systems was found to be comparable although the degradation rate was slightly lower with immobilized cells. The most important result was the finding that the positive effect of FeNTA on ABT degradation with immobilized cells was similar to that observed previously with free cells. All these results show that mechanisms observed with free cells can be extrapolated to entrapped cells, i.e. under conditions much closer to those usually encountered in the environment.

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Section: Introductionmentioning

confidence: 99%

2-Aminobenzothiazole degradation by free and Ca-alginate immobilized cells of Rhodococcus rhodochrous

et al. 2009

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“…Other studies describe the performance in 10 active cycles of phenol biodegradation by immo bilized in Ca alginate Rhodococcus sp. cells (Pai et al, 1995). Quek et al (2006), reported on the immobiliza tion and performance of a hydrocarbondegrading Rhodococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Hristov

Christova

Kabaivanova

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tThe capability of the biosurfactantproducing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by Rhodococcus free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l nhexadecane (40 cycles). The alkanotrophic strain R. wratislawiensis BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous supermacroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water. 288of materials due to their unique heterogeneous open porous structure, which significantly increases the equilibrium sorption properties and allows unhindered diffusion of solutes, nanoparticles and microparticles. Usually, cryogels possess spongylike structure of huge pores (50-200 µm) containing free water surrounded by thin walls and, therefore, they are often used for immo bilization of enzymes and cells by entrapment inside the channels of interconnected pores (Lozinsky et al., 2003).Many bacterial strains have been isolated with the abilities to degrade nhexadecane and phenol sepa rately, which are often used to represent the aliphatic and aromatic pollutant (Yordanova et al., 2009; Abdel Megeed et al., 2010;Tambekar et al., 2012). However, few strains have been reported to have the dual abilities (Sun et al., 2012).This paper aims to report the first study on simul taneous biodegradation of nhexadecane and phenol by Rhodococcus wratislawiensis BN38 immobilized in hydroxypropylcellulose/poly (Nisopropylacrylamide) cryogel matrix. Experimental Materials and MethodsMicroorganism, media and cultivation. The R. wrati slawiensis BN38, employed in this study was isolated from soil polluted with hydrocarbons by a standard enrichment technique (Tuleva et al., 2008 O, 0.2 and phenol and nhexadecane at 500 mg/l, unless otherwise mentioned, was used. The pH of the medium was adjusted to 7.0. The MSM was solidified as MSM phenol agar by addition of 1.8% agar when necessary. Cultures grown on MSM agar supplemented with 500 mg/l phenol were used to inoculate 500 ml Erlenmeyer flasks, containing 100 ml liquid MSM. At each cycle MSM was supplemented to 100 ml with fresh sterile medium without cell transfer and only phenol was added. Cultures were incubated during the longterm semicontinuous biodegradation processes while shaking (120 rpm) at 29°C. Inocula with cell density (OD 610 nm) of 0.4 were employed in the processes of biodegradation. The pH values were 6.7-6.8 all the time, due to the buffering activity of the nutrient medium used.Bacterial growth was assessed by determination of the optical density (OD 610 nm) of t...

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“…Subsequently, researchers can use it for the indication of phenolic compounds biodegradation. A number of researchers reported that once the initial phenolic compounds concentration rises a slight reduction of phenol degradation is observed as pH variation increases (Monteiro et al, 2000;Pai et al, 1995). Annadurai et al (2002) have reported that the pH medium considerably affects the phenolic compounds biodegradation; experiments with Pseudomonas putida indicated that it could not resist changing in pH efficiently.…”

Section: Kinetics and Effects Of Phmentioning

confidence: 99%

Kinetic Parameters of Phenol Biodegradation With Different Microorganisms: A Review

Mizzouri

2017

juod

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Most industries release wastewater contains high concentrations of phenol which is toxic and contaminating the environment. Biological treatments are preferable for the phenolic compounds treatment. This review aims to investigate the impacts of temperatures, pH, and concentration of substrate on the phenolic compounds biodegradation, to compare the kinetic parameters of different microorganisms, and to discuss the different between the kinetic parameters of aerobic and anaerobic treatment. The review showed that most of the phenol biodegrading bacteria are P. Putida species and mixed cultures but P. Putida has a better adaptation to phenol biodegradation. The values of µ max and K s in anaerobic process are smaller than the values attained in aerobic process. The optimum temperature to acclimatize bacteria to the phenol substrate is 30 ºC while the optimum pH condition is between 6.5 and 7.5. As the phenol concentration was increased, there was an increase in the values of the Ks and even when concentration is low; phenols have a significant inhibitory impact on (μ). The values of Ki for phenol degradation for P. putida species were higher than the values of Ki of mixed cultures. The highest Ki value for the phenol degrading among P.Putida species was 1185.8 mg/L and the highest Ki value among mixed cultures was 648.1 mg/L and the highest Ki value among the other species was 2434.7 mg/L.

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Continuous degradation of phenol by Rhodococcus sp. immobilized on granular activated carbon and in calcium alginate

Cited by 65 publications

References 7 publications

2-Aminobenzothiazole degradation by free and Ca-alginate immobilized cells of Rhodococcus rhodochrous

2-Aminobenzothiazole degradation by free and Ca-alginate immobilized cells of Rhodococcus rhodochrous

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Kinetic Parameters of Phenol Biodegradation With Different Microorganisms: A Review

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