Continuous cyclic stretch induces osteoblast alignment and formation of anisotropic collagen fiber matrix

Matsugaki, Aira; Fujiwara, Natsuko; Nakano, Takayoshi

doi:10.1016/j.actbio.2013.03.015

Cited by 89 publications

(76 citation statements)

References 43 publications

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“…Tissue deformation that occurs naturally in muscular and cardiovascular systems regulates various tissue morphogenic processes. 95,96 Mechanical stretching of rat microvascular endothelial cells enhanced the production of MMP-2, MT1-MMP, HIF-1α, HIF-2α, and VEGF at both the transcriptional and translational levels. 97 Stretching also induced an increase in intracellular Ca 2+ concentration and traction forces of NIH-3T3 fibroblasts via stretch-activated ion channels.…”

Section: Mechanical Perturbationmentioning

confidence: 99%

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

et al. 2015

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Section: Mechanical Perturbationmentioning

confidence: 99%

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

et al. 2015

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“…This observation indicates that although surface topography induces cellular elongation parallel to the substrate orientation, as mechanical loading does in the direction of the applied load [129,130], surface-induced cellular elongation does not activate apoptotic pathways, as mechanical tension may bring about [131][132][133][134].…”

Section: Discussionmentioning

confidence: 85%

The Influence of Anisotropic Nano- To Micro-Topography on in Vitro and in Vivo Osteogenesis

Azeem

English

Kumar

et al. 2015

Nanomedicine (Lond.)

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“…Since cell orientation is a crucial factor for construction of the anisotropic bone architecture, 5,18,19) directional control of osteoblast was driven by oriented collagen substrates. In contrast to the rounded shape of osteoblasts observed on the cover glass (Fig.…”

Section: Directional Control Of Juvenile Osteoblasts For Con-mentioning

confidence: 99%

Directional Control of Mature Osteoblast Derived from Juvenile Mouse Calvariae

Ozasa

Matsugaki

Isobe³

et al. 2017

Mater. Trans.

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Bone tissue has a highly anisotropic microstructure comprised of biological apatite (BAp) and collagen brils, which is closely related to bone mechanical function. The formation of anisotropic bone microstructure is governed by bone-forming osteoblasts; therefore, isolation of functional osteoblasts and regulation of their arrangement for generating bone tissue with optimally-oriented microstructure during bone reconstruction are important. In this study, we established the isolation and culture conditions of mature osteoblasts derived from juvenile mice (2-week-old). Osteoblasts from juvenile mice expressed signi cantly higher level of osteoblastic markers (alkaline phosphatase, osterix, and osteocalcin) than osteoblasts from neonatal mice, indicating that juvenile osteoblasts are promising materials for bone tissue engineering. Moreover, alignment of the mature osteoblasts was successfully regulated using oriented collagen substrates. This is the report that shows directional control of mature osteoblasts isolated from juvenile mice.

show abstract

Continuous cyclic stretch induces osteoblast alignment and formation of anisotropic collagen fiber matrix

Cited by 89 publications

References 43 publications

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

Advances in Techniques for Probing Mechanoregulation of Tissue Morphogenesis

The Influence of Anisotropic Nano- To Micro-Topography on in Vitro and in Vivo Osteogenesis

Directional Control of Mature Osteoblast Derived from Juvenile Mouse Calvariae

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