Continuous Crosscurrent Extraction of Proteins in Process Scale

Hustedt, Helmut; Kroner, K. H.; Papamichael, N.

doi:10.1007/978-1-4684-5667-7_49

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…Also shown in the figure is a straight line with slope V,, for the profile of stability ratio 0.18, which corresponds to the definition in Eq. (5). It is obvious, from the graph, that this is a rough approximation as V, corresponds to an overall velocity of settling.…”

Section: Pure Systemsmentioning

confidence: 91%

Kinetics of phase separation for polyethylene glycol–phosphate two‐phase systems

Kaul

Pereira

Asenjo

et al. 1995

Biotech & Bioengineering

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Phase separation times for polyethylene glycol (PEG)-4000-phosphate aqueous two-phase systems were studied, for small scale (5-g) and large scale (1300-g) systems, as a -function of the stability ratio. Profiles of dispersion height for both large and small scale systems were represented as a fraction of the initial height and were found to be independent of the geometrical dimensions of the separator. Furthermore, by plotting time as a fraction of the initial height the total time of separation can be calculated for a given height of system at a particular stability ratio. This generalization is important for the design of large scale aqueous two-phase separators. Phase separation times were also found to be dependent on which of the phases is continuous. A characteristic change in phase separation time was also observed at the phase inversion point (i.e., where the dispersed phase changes to a continuous phase and vice versa) and this point tends toward higher volume ratios as the tie-line length (TLL) is increased. Furthermore, the phase inversion point at each TLL corresponds to a fixed phosphate concentration.

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Section: Pure Systemsmentioning

confidence: 91%

Kinetics of phase separation for polyethylene glycol–phosphate two‐phase systems

Kaul

Pereira

Asenjo

et al. 1995

Biotech & Bioengineering

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“…ATPS has been used to demonstrate the isolation and purification of a variety of proteins [1,4]. It appears to be easier to scale up than other processes [9,95,96]. It is worth noting that the partition coefficient is unaffected by the initial protein concentration throughout a wide concentration range and operation scale.…”

Section: Extractions Using Atpsmentioning

confidence: 99%

Theoretical Aspects and Applications of Aqueous Two‐Phase Systems

Singla

Sit

2022

ChemBioEng Reviews

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The aqueous two-phase system (ATPS) is commonly known as a technique that yields high-purity products in a single step. It is particularly advantageous for purifying biomolecules like proteins, nucleic acids, enzymes, viruses etc. Currently, aqueous two-phase extraction (ATPE), i.e., liquid-liquid extraction, involves the transfer of the solute from one aqueous phase to another. In ATPE, for recovery of biomolecules, polymer-polymer and polymersalt type systems are used. The most recent developments with respect to recovery of biomolecules by ATPS are reviewed and discussed, covering the mechanism, which controls the phase formation, the conditions of solute partitioning in ATPS processes, and factors influencing the ATPS including concentration and molecular weight (MW) of polymers, types of salt, pH, and temperature. In addition, also the increasing applications of ATPS for the recovery of high-value bioproducts, the benefits of the ATPS recovery system, and the recent developments of alternative low-cost ATPS are highlighted.

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“…It is affected by various parameters such as pH, molar mass, concentration of PEG, type and concentration of salt, and properties of the protein 2. The ATPS made of PEG and salt is highly preferable compared to a PEG‐polymer system because of low cost and, in addition, it allows the scale‐up for continuous industrial operation 5, 6. Citrate 7, phosphate 8, 9, and sulfate 10 salts are well studied for partitioning of enzymes in an ATPS but the use of carbonate as a bottom phase for such purpose is not reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Rhizopus niveus Lipase Partitioning by an Aqueous Biphasic System

et al. 2014

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An unconventional aqueous two‐phase system (ATPS) composed of polyethylene glycol (PEG) and sodium carbonate was developed and optimized by employing response surface methodology for separation of Rhizopus niveus lipase. A five‐level central composite design was applied to evaluate the optimal level of three process variables in order to obtain maximum lipase separation. Experimental data were analyzed by regression and a polynomial model was created which was found significant. The maximum partition coefficient was achieved with the system PEG 4000/sodium carbonate. Validation experiments confirmed the high accordance of predicted and experimental results. The optimized ATPS can be applied as a suitable cost‐effective system for lipase extraction.

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Continuous Crosscurrent Extraction of Proteins in Process Scale

Cited by 3 publications

References 4 publications

Kinetics of phase separation for polyethylene glycol–phosphate two‐phase systems

Kinetics of phase separation for polyethylene glycol–phosphate two‐phase systems

Theoretical Aspects and Applications of Aqueous Two‐Phase Systems

Optimization of Rhizopus niveus Lipase Partitioning by an Aqueous Biphasic System

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