Continuous biomass and lipid production from local chlorella-bacteria consortium in raw wastewater using volatile fatty acids

To evaluate the suitability of wastewater on the growth of alga Spirulina, reclaimed wastewaters, fishpond wastewater, industrial wastewater and mariculture water were used to culture Spirulina subsalsa and Spirulina platensis. S. subsalsa showed better adaptability to fishpond wastewater and higher specific growth rate than that of S. platensis. Thus, effects of factors such as the amount of baking soda, inoculation, and sodium nitrate on the growth, biomass and protein content of S. subsalsa were evaluated through the single factors design. Results showed that S. subsalsa had good growth under conditions of 4-12 g/L baking soda, 1-2 g/L NaNO 3 , 25-40°C, and 6000-12000 lux illumination. The maximum dry biomass and protein content were 3.48 g/L and 33.08%, respectively, suggesting that it was feasible to culture S. subsalsa in freshwater aquaculture wastewater.

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“…To further reduce the cost of management, application of wastewater to culture microalgae becomes a promising way recently [7,8].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of common wastewaters on the growth of alga Spirulina

Wongsnansilp¹,

Phinrub²

2022

J App Biol Biotech

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Biochar-derived dissolved organic matters influencing bacterium characteristics during biodegradation of sulfamethoxazole and chloramphenicol under alternation of visible and avoiding light

Yang

Xue²,

Gao³

et al. 2023

Biochar

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The influence of biochar-released dissolved organic matter (BDOM) on the transcription of gene (DEG) in Pseudomonas stutzeri and Shewanella putrefacien during sulfamethoxazole (SMX) and chloramphenicol (CAP) biodegradation under visible light was investigated in this study. The results indicated that BDOM components would be nutrients for bacterial amplification and growth under the culture conditions of xenon lamp irradiation and avoiding light, especially BDOM from low temperatures. Additionally, visible light irradiation would improve the saturated fatty acid by stimulating the cell membrane of the microorganism, thus promoting the biodegradation of antibiotics through altering P. stutzeri and S. putrefaciens reoxidative and catabolism processes and significantly inhabiting the copy number of their genes. Moreover, the upregulated genes and enzymes related to SMX and CAP-metabolic and catabolic processes were enriched, which were involved in the pathways of biodegradation, further improving biodegradation efficiency. In particular, interaction network analysis between the top 100 dominant functional genes from P. stutzeri and S. putrefaciens and the molecular types of BDOM, e.g., CHO, CHON, and CHOS (p < 0.05), indicated that the genes of molecular function showed a high positive or negative correlation with the CHO type of BDOM. The results revealed that the CHO type of BDOM affected the functional genes of molecular function, cellular component, and biological process from P. stutzeri and S. putrefaciens, influencing the biodegradation of SMX and CAP. This study provided an basis for BDOM playing a role in antibiotic removal from the aqueous solution using biochar combined with photobiodegradation. Graphical Abstract

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