Continuous beer fermentation by high cell-density culture of bottom brewer's yeast

Okabe, Mitsuyasu; Oda, Atsushi; Park, Yong Soo; Noguchi, Katsuhiro; Okamoto, Takanori; Mitsui, Shunsuke

doi:10.1016/0922-338x(94)90206-2

Cited by 7 publications

(3 citation statements)

References 4 publications

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“…Squares, OD 600 ; diamonds, acetic acid; triangles, formic acid is not an insignificant economical factor: implementing high cell densities in a later fermentation process is considered to be one of the most effective ways for enhancing the productivity [40]. Efficient cell recycling and cell retention systems with optimized conditions for the accumulation of high cell densities up to 200 g/L were already implemented in bioprocesses [41][42][43].…”

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confidence: 99%

Whole-cell biocatalysis for hydrogen storage and syngas conversion to formate using a thermophilic acetogen

Schwarz

Müller

2020

Biotechnol Biofuels

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Background: In times of global climate change, the conversion and capturing of inorganic CO 2 have gained increased attention because of its great potential as sustainable feedstock in the production of biofuels and biochemicals. CO 2 is not only the substrate for the production of value-added chemicals in CO 2-based bioprocesses, it can also be directly hydrated to formic acid, a so-called liquid organic hydrogen carrier (LOHC), by chemical and biological catalysts. Recently, a new group of enzymes were discovered in the two acetogenic bacteria Acetobacterium woodii and Thermoanaerobacter kivui which catalyze the direct hydrogenation of CO 2 to formic acid with exceptional high rates, the hydrogen-dependent CO 2 reductases (HDCRs). Since these enzymes are promising biocatalysts for the capturing of CO 2 and the storage of molecular hydrogen in form of formic acid, we designed a whole-cell approach for T. kivui to take advantage of using whole cells from a thermophilic organism as H 2 /CO 2 storage platform. Additionally, T. kivui cells were used as microbial cell factories for the production of formic acid from syngas. Results: This study demonstrates the efficient whole-cell biocatalysis for the conversion of H 2 + CO 2 to formic acid in the presence of bicarbonate by T. kivui. Interestingly, the addition of KHCO 3 not only stimulated formate formation dramatically but it also completely abolished unwanted side product formation (acetate) under these conditions and bicarbonate was shown to inhibit the membrane-bound ATP synthase. Cell suspensions reached specific formate production rates of 234 mmol g −1 protein h −1 (152 mmol g −1 CDW h −1), the highest rates ever reported in closed-batch conditions. The volumetric formate production rate was 270 mmol L −1 h −1 at 4 mg mL −1. Additionally, this study is the first demonstration that syngas can be converted exclusively to formate using an acetogenic bacterium and high titers up to 130 mM of formate were reached. Conclusions: The thermophilic acetogenic bacterium T. kivui is an efficient biocatalyst which makes this organism a promising candidate for future biotechnological applications in hydrogen storage, CO 2 capturing and syngas conversion to formate.

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confidence: 99%

Whole-cell biocatalysis for hydrogen storage and syngas conversion to formate using a thermophilic acetogen

Schwarz

Müller

2020

Biotechnol Biofuels

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“…Other continuous cultivation systems employ a cell retention system when the maximum division rate of a given strain in given conditions is found to be below the needed dilution rate. If a continuous culture cell retention system is in operation, also a steady state can be reached through setting up a feed, bleed, and cell recycling system (Okabe et al 1994; Richter and Nottelmann 2004; Deschênes et al 2006). When a population of cells is grown in steady-state mode, which means growth at a fixed average μ , even though not all continuous cultures can be operated or are able to reach a steady state.…”

Section: Anaerobic Microbiology and Biomass Cultivation Techniquesmentioning

confidence: 99%

Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology

et al. 2018

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Anaerobic microorganisms (anaerobes) possess a fascinating metabolic versatility. This characteristic makes anaerobes interesting candidates for physiological studies and utilizable as microbial cell factories. To investigate the physiological characteristics of an anaerobic microbial population, yield, productivity, specific growth rate, biomass production, substrate uptake, and product formation are regarded as essential variables. The determination of those variables in distinct cultivation systems may be achieved by using different techniques for sampling, measuring of growth, substrate uptake, and product formation kinetics. In this review, a comprehensive overview of methods is presented, and the applicability is discussed in the frame of anaerobic microbiology and biotechnology.

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“…In such cases, the time to eliminate diacetyl remains long, reducing strongly the advantage of accelerated primary fermentation. Another process of accelerated fermentation consists of heating the green beer to 60−90 °C for 5−60 min (5–7) before the maturation step (Figure 1b). The aim of heating is to quickly convert α‐acetolactate into diacetyl/acetoin.…”

Section: Introductionmentioning

confidence: 99%

Improved Performances and Control of Beer Fermentation Using Encapsulated α-Acetolactate Decarboxylase and Modeling

Dulieu

Moll²,

Boudrant

et al. 2000

Biotechnol. Prog.

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The use of the enzyme alpha-acetolactate decarboxylase allows the acceleration of beer fermentation/maturation because it shunts diacetyl formation, whose elimination is the rate-limiting step of the process. To obtain a cost reduction by using this exogenous enzyme, we propose a new process involving recoverable encapsulated alpha-acetolactate decarboxylase. The performance of traditional and new processes was investigated by a modeling approach. A simple model, focused on alpha-acetolactate and diacetyl profiles during beer fermentation, was set up. The simulated profiles are consistent with literature data. This study shows also that encapsulated alpha-acetolactate decarboxylase allows the acceleration of beer fermentation as efficiently as free alpha-acetolactate decarboxylase. The advantage of immobilized alpha-acetolactate decarboxylase versus free enzyme is that it is recoverable and reusable, which means a process cost reduction.

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Continuous beer fermentation by high cell-density culture of bottom brewer's yeast

Cited by 7 publications

References 4 publications

Whole-cell biocatalysis for hydrogen storage and syngas conversion to formate using a thermophilic acetogen

Whole-cell biocatalysis for hydrogen storage and syngas conversion to formate using a thermophilic acetogen

Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology

Improved Performances and Control of Beer Fermentation Using Encapsulated α-Acetolactate Decarboxylase and Modeling

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