Continuous beds: high-resolving, cost-effective chromatographic matrices

Hjertén, Stellan; Li, Yaxue; Liao, Jiali; Mohammad, Jamil; Nakazato, Kenichi; Pettersson, Göran

doi:10.1038/356810a0

Cited by 166 publications

(73 citation statements)

References 14 publications

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“…2). In agreement with previous experiments on compressed beds of nonporous agarose beds (10) and of continuous beds (11), the resolution increases with a dilution of the Polybuffer, which is in contrast to what has been found for chromatofocusing on the Polybuffer exchanger PBE 94 (12).…”

Section: Resultssupporting

confidence: 88%

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Continuous Beds for Microchromatography: Chromatofocusing and Anion Exchange Chromatography

Liao

Zhang

et al. 1999

Analytical Biochemistry

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Section: Resultssupporting

confidence: 88%

“…Microcolumns prepared by the present method adsorbed 13 g of cellulase per microliter bed. For human hemoglobin adsorbed to a compressed continuous bed the corresponding figure was 12 (11).…”

Section: Resultsmentioning

confidence: 99%

Continuous Beds for Microchromatography: Chromatofocusing and Anion Exchange Chromatography

Liao

Zhang

et al. 1999

Analytical Biochemistry

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“…HjertØn et al [1,2] were the first in 1989 to report a continuousbed column made of compressed polyacrylamide. Since then, numerous groups have developed organic polymer monoliths using radical polymerization techniques with different monomer compositions [2 -10].…”

Section: Introductionmentioning

confidence: 99%

Applications of silica‐based monolithic HPLC columns

Cabrera

2004

J of Separation Science

398

226

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The recent invention and successive commercial introduction of monolithic silica columns has motivated many scientists from both academia and industry to study their use in HPLC. The first paper on monolithic silica columns appeared in 1996. Currently about 200 papers have been published relating to applications and characterization of monolithic silica columns, including monolithic capillaries. This review attempts to give an overview covering various aspects of this new column type in the field of high throughput analysis of drugs and metabolites, chiral separations, analysis of pollutants and food-relevant compounds, as well as in bioanalytical separations such as in proteomics. Some of the applications are described in greater detail. The numerous publications dealing with the physicochemical and chromatographic characterization of monolithic silica columns are briefly summarized.

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“…There are many kinds of continuous media [2 -9], and one of them is continuous column [8,9]. Swollen polyacrylamide gel is compressed in the shape of a column and forms a continuous column, and can be used successfully for a style of HPLC to separate proteins and peptides [8, 10 -12].…”

Section: Introductionmentioning

confidence: 99%

Preparation of a monolithic column for weak cation exchange chromatography and its application in the separation of biopolymers

Wei¹,

Huang

Liu

et al. 2006

J of Separation Science

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Original PaperPreparation of a monolithic column for weak cation exchange chromatography and its application in the separation of biopolymers A procedure for the preparation of a monolithic column for weak cation exchange chromatography was presented. The structure of the monolithic column was evaluated by mercury intrusion. The hydrodynamic and chromatographic properties of the monolithic column -such as back pressures at different flow rates, effects of pH on protein retention, dynamic loading capacity, recovery, and stability -were determined under conditions typical for ion-exchange chromatography. The prepared monolithic column might be used in a relatively broad pH range from 4.0 to 12.0 and exhibited an excellent separation to five proteins at the flow rates of both 1.0 and 8.0 mL/min, respectively. In addition, the prepared column was first used in the purification and simultaneous renaturation of recombinant human interferon gamma (rhIFN-c) in the extract solution with 7.0 mol/L guanidine hydrochloride. The purity and specific bioactivity of the purified rhIFN-c in only one chromatographic step were obtained to be 93% and 7.8610 7 IU/mg, respectively.

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Continuous beds: high-resolving, cost-effective chromatographic matrices

Cited by 166 publications

References 14 publications

Continuous Beds for Microchromatography: Chromatofocusing and Anion Exchange Chromatography

Continuous Beds for Microchromatography: Chromatofocusing and Anion Exchange Chromatography

Applications of silica‐based monolithic HPLC columns

Preparation of a monolithic column for weak cation exchange chromatography and its application in the separation of biopolymers

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