Continuous Beds for Microchromatography: Cation-Exchange Chromatography

Li, Yiming; Liao, Jiali; Nakazato, Kenichi; Mohammad, Jamil; Terenius, Lars; Hjertén, Stellan

doi:10.1006/abio.1994.1561

Cited by 72 publications

(52 citation statements)

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“…Polymerization can be carried out directly in the column [63][64][65][66][67] or membranes can be prepared from a suitable polymer, placed in a cartridge and used for rapid separations [68]. These media are primarily intended for fast separations of proteins, but bradykinin and D-Phe7 bradykinin can also be separated within 3 min with a sufficient efficiency of 97 900 plates/m [65].…”

Section: Supports and Their Modificationmentioning

confidence: 99%

“…These media are primarily intended for fast separations of proteins, but bradykinin and D-Phe7 bradykinin can also be separated within 3 min with a sufficient efficiency of 97 900 plates/m [65]. Continuous beds for IEC have been prepared in fused silica tubings of up to 10 urn in diameter [66]. The beds are produced in the form of rods traversed by channels through which the eluent passes.…”

Section: Supports and Their Modificationmentioning

confidence: 99%

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Stationary phases for peptide analysis by high performance liquid chromatography: a review

Štulı́k¹,

Pacáková²,

Suchánková³

et al. 1997

Analytica Chimica Acta

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A survey is given of modern stationary phases employed in high performance liquid chromatography (HPLC) analysis of peptides. The physico-chemical properties of peptides and their consequences for the selection and optimization of the separation system are briefly discussed, followed by a summary of the approaches to the selection and characterization of stationary phases. The properties and applicability of various stationary phases are then critically reviewed, including aspects such as size-exclusion, ion-exchange, reversed-phase, hydrophobic-interaction, affinity and chiral systems, as well as some specialized separation techniques. Emphasis is placed on the most recent literature. 0 1997 Elsevier Science B.V.

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Section: Supports and Their Modificationmentioning

confidence: 99%

Section: Supports and Their Modificationmentioning

confidence: 99%

Stationary phases for peptide analysis by high performance liquid chromatography: a review

Štulı́k¹,

Pacáková²,

Suchánková³

et al. 1997

Analytica Chimica Acta

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“…Following polymerization the column was equilibrated with 0.02 M sodium phosphate, pH 6.2. All details of this very simple and cost-effective method for the preparation of the micro column are described in [6]. Observe that no frit is required to support this so-called continuous bed (a polymer rod with channels through which the eluent can pass) since it is attached covalently to the wall of the fused silica tubing.…”

mentioning

confidence: 99%

Solid phase micro extraction of biopolymers, exemplified with adsorption of basic proteins onto a fiber coated with polyacrylic acid

et al. 1996

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Fused silica fibers coated with polyacrylic acid were used to extract proteins. The amount of protein extracted is proportional to the concentration of the protein in the sample for very short extraction times (a few seconds) when the binding capacity of the coated fiber for proteins is low. Proportionality is also obtained when the extraction time is extended to several minutes, provided that the binding capacity exceeds the protein content of the sample. However, when the capacity is low these long extraction times favor the extraction of the more strongly adsorbed proteins. The mixture of extracted proteins was analyzed by micro‐HPLC using columns based on a new continuous polymer bed technology. © 1996 John Wiley & Sons, Inc.

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“…Capillary columns offer some distinct advantages over standard HPLC columns [1,2]. These include the reduction of the sample sizes and solvent consumption, and increasing of the detection limit by using concentration sensitive detection devices without splitting, such as mass spectrometers or flame-based detectors.…”

Section: Introductionmentioning

confidence: 99%

“…The same technology can also be applied in the preparation of capillary columns, and supporting frits are not required to retain the packed beds. Therefore, monolithic capillary columns have encountered wide interest for potential applications in micro liquid chromatography and capillary electrochromatography (CEC) [5][6][7][8][9][10][11]. As the porous properties of the monolithic polymers can be easily tuned, macroporous rods with large through-pore diameters above 1000 nm and high porosity ratio can be produced.…”

Section: Introductionmentioning

confidence: 99%