Continuous and Batch Production of Chloroperoxidase by Mycelial Pellets of
            <i>Caldariomyces fumago</i>
            in an Airlift Fermentor

Carmichael, Robert D.; Pickard, Michael A.

doi:10.1128/aem.55.1.17-20.1989

Cited by 26 publications

(4 citation statements)

References 18 publications

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“…For Caldariomyces fumago , aforementioned relationships were also found. Additionally, pellet density was reported to be inversely related to pellet size (Carmichael and Pickard 1989 ).…”

Section: Spore Inoculum Level and Other Inoculation Strategiesmentioning

confidence: 99%

The filamentous fungal pellet—relationship between morphology and productivity

Veiter¹,

Rajamanickam²,

Herwig³

2018

Appl Microbiol Biotechnol

181

133

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Filamentous fungi are used for the production of a multitude of highly relevant biotechnological products like citric acid and penicillin. In submerged culture, fungi can either grow in dispersed form or as spherical pellets consisting of aggregated hyphal structures. Pellet morphology, process control and productivity are highly interlinked. On the one hand, process control in a bioreactor usually demands for compact and small pellets due to rheological issues. On the other hand, optimal productivity might be associated with less dense and larger morphology. Over the years, several publications have dealt with aforementioned relations within the confines of specific organisms and products. However, contributions which evaluate such interlinkages across several fungal species are scarce. For this purpose, we are looking into methods to manipulate fungal pellet morphology in relation to individual species and products. This review attempts to address (i) how variability of pellet morphology can be assessed and (ii) how morphology is linked to productivity. Firstly, the mechanism of pellet formation is outlined. Subsequently, the description and analysis of morphological variations are discussed to finally establish interlinkages between productivity, performance and morphology across different fungal species.

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“…For Caldariomyces fumago , aforementioned relationships were also found. Additionally, pellet density was reported to be inversely related to pellet size (Carmichael and Pickard 1989 ).…”

Section: Spore Inoculum Level and Other Inoculation Strategiesmentioning

confidence: 99%

The filamentous fungal pellet—relationship between morphology and productivity

Veiter¹,

Rajamanickam²,

Herwig³

2018

Appl Microbiol Biotechnol

181

133

View full text Add to dashboard Cite

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“…The MCD was synthesized as described by Hager et al [12] and molar extinction coefficient was determined to be 1.21 × 10 4 L mol −1 cm −1 . Pigment absorption was measured spectroscopically at 575 nm in 20 mM potassium phosphate buffer pH 5.5 [16]. Determination of protein concentration was performed spectrophotometrically at 595 nm as described by Bradford [17] using bovine serum albumin as standard.…”

Section: Analysesmentioning

confidence: 99%

Chloroperoxidase production by Caldariomyces fumago biofilms

Huster

Müller‐Renno

Ziegler

et al. 2015

Engineering in Life Sciences

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Chloroperoxidase (CPO) is a versatile enzyme, which is secreted by the marine fungus Caldariomyces fumago (Leptoxyphium fumago). However, the application of the enzyme is hampered by its high price, which is due to the costly, labor‐intensive purification process. One challenge of the downstream process is the removal of a coproduced black pigment that forms a complex with the active enzyme. While strain development can be considered as an option to reduce the synthesis of the interfering pigment, the metabolism of the microorganism can be altered alternatively by using the biofilm growth mode of the fungus. The aim of this study was to reduce pigment formation during CPO synthesis. We investigated for the first time CPO production during C. fumago biofilm growth initiated through the presence of different microstructured stainless steel surfaces (material number: 1.4571; AISI 316Ti). CPO production by C. fumago was similar when grown as a biofilm or in suspension, whereas pigment formation was drastically reduced by cells grown on moderately structured surfaces (Ra = 0.13 ± 0.02 μm). The possibilities of biofilm growth for changing cell properties and for continuous fermentation are discussed.

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“…Like most peroxidases, CPO catalyzes the peroxide-dependent oxidation of a wide spectrum of organic and inorganic compounds (24). CPO can be produced by C. fumago grown in a defined fructose-salts medium, in a semicontinuous or continuous manner, and at up to 0.5 to 0.8 g of enzyme per liter (2,5,6). Under these conditions, it is the only protein secreted and is readily purified from the culture medium.…”

mentioning

confidence: 99%

Immobilization of Chloroperoxidase on Aminopropyl-Glass

Kadima

Pickard

1990

Appl Environ Microbiol

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Chloroperoxidase (CPO) purified from Caldariomyces fumago CMI 89362 was covalently bound to aminopropyl-glass by using a modification of an established method. Acid-washed glass was derivatized by using aminopropyltriethoxysilane, and the enzyme was ionically bound at low ionic strength. Further treatment with glutaraldehyde covalently linked the enzyme to the glass beads in an active form. No elution of bound activity from glass beads could be detected with a variety of washings. The loading of enzyme protein to the glass beads was highest, 100 mg of CPO per g of glass, at high reaction ratios of CPO to glass, but the specific activity of the immobilized enzyme was highest, 36% of theoretical, at low enzyme-to-carrier ratios. No differences in the properties of the soluble and immobilized enzymes could be detected by a number of criteria: their pH-activity and pH-stability profiles were similar, as were their thermal stabilities. After five uses, the immobilized enzyme retained full activity between pH 6.0 and 6.7.

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Continuous and Batch Production of Chloroperoxidase by Mycelial Pellets of Caldariomyces fumago in an Airlift Fermentor

Cited by 26 publications

References 18 publications

The filamentous fungal pellet—relationship between morphology and productivity

The filamentous fungal pellet—relationship between morphology and productivity

Chloroperoxidase production by Caldariomyces fumago biofilms

Immobilization of Chloroperoxidase on Aminopropyl-Glass

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