Continuous Anaerobic Digestion of Wood Vinegar Wastewater From Pyrolysis: Microbial Diversity and Functional Genes Prediction

Abstract

Wood vinegar wastewater (WVWW) is the main by-product of biomass pyrolysis process, which is more suitable to use anaerobic digestion (AD) to achieve energy recovery due to its large amount of organic matter. In this study, the up-flow anaerobic sludge bed (UASB) reactor was used to investigate the continuous anaerobic transformation of WVWW with gradient concentrations (0.3, 0.675, 1, 2, 3, 4, 5, 6, and 7 g COD/L). Then, the changes of microbial community, diversity index and functional gene were analyzed in detail. The results revealed that WVWW showed good AD performance in continuous fermentation. WVWW of organic loading rate (OLR) of >8.58 g COD/L•d showed severe inhibition on biodegradability and methane production, which is mainly due to the toxic substances as compared with the control group. The bacterial communities were dominated by phyla of Chloroflexi, Firmicutes, Proteobacteria, Acidobacteria, Synergistetes, and Actinobacteria. The gene abundances related to energy production, carbohydrate transport and metabolism were relatively high, which are mainly responsible for carbon forms conversion and carbohydrate degradation. This study will provide a basis for the screening and enrichment of functional bacteria and genes.

Synergistic Effects of Catalyst Mixtures on Biomass Catalytic Pyrolysis

“…The challenge remains on how to upgrade pyrolysis bio-oil to drop-in liquid fuels due to their high oxygen content, high viscosity, and acidity. Several techniques have been investigated, including the use of catalytic materials and microwave heating, to enhance the quality of bio-oil and biochar ( Budarin et al, 2009 ; Luque et al, 2012 ; Bundhoo, 2018 ; State et al, 2019 ; Hua et al, 2020 ). Catalytic fast pyrolysis has been found to be effective in improving the quality of bio-oil ( Carlson et al, 2011 ; Rezaei et al, 2014 ; Bundhoo, 2018 ; Song et al, 2020 ; Yu et al, 2020 ).…”

Sustainable Plant Growth Promotion and Chemical Composition of Pyroligneous Acid When Applied with Biochar as a Soil Amendment

“…PA is composed of water (80–90%) and more than 200 organic compounds, including acids, alcohols, phenols, aldehydes, and esters (10–20%) depending on pyrolysis conditions [ 1 , 2 , 3 , 4 , 5 ]. Some of these compounds have antioxidant properties and can be developed into a range of useful products [ 6 , 7 , 8 , 9 , 10 ].…”

“…This work reports on the effect of combined applications of chemical fertilizer, biochar, and PA on plant growth in a pot experiment (treatments described in Table 1 ). To characterize chemical profiles of the organic inputs, four spectroscopic measurements of fluorescence excitation were utilized—excitation-emission matrix (EEM) [ 5 , 10 ], ion chromatography [ 26 ], high-performance liquid chromatography (HPLC) [ 20 ], and gas chromatography-mass spectrometry (GC-MS) [ 8 , 26 , 27 ]. The effect of inputs on growth of Komatsuna plants ( Brassica rapa var.…”

Data_Sheet_1.docx

“…The challenge remains on how to upgrade pyrolysis bio-oil to drop-in liquid fuels due to their high oxygen content, high viscosity, and acidity. Several techniques have been investigated, including the use of catalytic materials and microwave heating, to enhance the quality of bio-oil and biochar (Budarin et al, 2009;Luque et al, 2012;Bundhoo, 2018;State et al, 2019;Hua et al, 2020). Catalytic fast pyrolysis has been found to be effective in improving the quality of bio-oil (Carlson et al, 2011;Rezaei et al, 2014;Bundhoo, 2018;Song et al, 2020;Yu et al, 2020).…”