Continuous acetone–butanol–ethanol production by corn stalk immobilized cells

Abstract

Corn stalk was used as a support to immobilize Clostridia beijerinckii ATCC 55025 in the fermentation process of acetone, butanol, and ethanol production. The effect of the dilution rate on solvent production was examined in a steady-state 20-day continuous flow operation. The maximum total solvent concentration of 8.99 g l(-1) was obtained at a dilution rate of 0.2 h(-1). Increasing the dilution rate between 0.2 and 1.0 h(-1) resulted in an increased solvent productivity, and the highest solvent productivity was obtained at 5.06 g l(-1) h(-1) with a dilution rate of 1 h(-1). The maximum solvent yield from glucose of 0.32 g g(-1) was observed at 0.25 h(-1). The cell adsorption and morphology change during the growth on corn stalk support were examined by the SEM.

Continuous two stage acetone–butanol–ethanol fermentation with integrated solvent removal using Clostridium acetobutylicum B 5313

“…A two-fold increase in productivity reduces capital expenditure by approximately 20% together with significant reductions in operating costs (Green, 2011). Zhang et al (2009) observed the highest solvent productivity and solvent yield as 5 g/L.h and 0.32 g/g with maximum dilution rate 1.0 1/h when cells were immobilized on corn stalk. The two-stage concept has also been successfully applied to other complex fermentation process such as methane production (Qureshi et al, 2000;Zhang et al, 2009).…”

“…Butanol extraction with liquid-liquid extraction has been reported to extract butanol from the fermentation broth without removing substrates, water or nutrients (Zhang, et al, 2009). Oleyl alcohol has been the extractant of choice among various researchers as it showed good extraction efficiency with non-toxicity towards the cells (Ezeji et al, 2006;Karcher et al, 2005).…”

Enhanced isopropanol–butanol–ethanol (IBE) production in immobilized column reactor using modified Clostridium acetobutylicum DSM792

“…The use of column reactors for continuous solvent production has been studied by several researchers using different immobilization materials and substrates [31][32][33]. However, the use of SEW spent liquor for the production of IBE solvents with modified strains has not been reported previously.…”

Can Microbially Derived Advanced Biofuels Ever Compete with Conventional Bioethanol? A Critical Review

“…Researchers have examined a range of biomass sources including corn stover, corn fiber, barley straw, and switch grass, as well as a variety of pretreatment conditions to assess their influence on n-butanol production (Parekh and Blaschek 1999;Parekh and Formanek 1999;Qureshi et al 1999;Qureshi et al 2006;Ezeji et al 2007;Zhang et al 2009;Qureshi et al 2010;Lin et al 2011;Mu et al 2011;Guo et al 2012Guo et al , 2013. Sugar concentrations ranged from 31 to 58 g/L with solvent titers ranging from 2 to 26 g/L (Parekh and Blaschek 1999;Parekh and Formanek 1999;Qureshi et al 1999Qureshi et al , 2006Ezeji et al 2007;Zhang et al 2009;Qureshi et al 2010;Lin et al 2011;Mu et al 2011;Guo et al 2012Guo et al , 2013. The highest yield reported was 0.44 g/g (Qureshi et al 2006(Qureshi et al , 2010, and the highest productivity was 5 g/L/h ).…”