Continued dominance of dengue virus serotype 2 during the recent Central India outbreaks (2019-2021) with evidence of genetic divergence

Agarwal, Ankita; Ganvir, R.U.C.H.I.; Kale, Dipesh; Chaurasia, Deepti; Kapoor, Garima

doi:10.1080/20477724.2023.2246712

Cited by 4 publications

(2 citation statements)

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“…of them belonged to DENV-2 serotype and of these 66 DENV isolates, 13 were sequenced of which 12 were found to belonged to genotype 4a during 2019 and 2021. It was also observed that the new lineage of DENV-2 was also associated with a longer duration of hospitalization (>10 ± 3 days) and hemorrhagic manifestations indicating its role in severity if disease in the studied area ( Agarwal et al, 2023 ). Higher evolutionary and increased transmission rates of one DENV genotype may outnumber the others in the community, resulting in a lineage shift.…”

Section: Discussionmentioning

confidence: 88%

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Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2

Sarma,

Rathod,

Mishra

et al. 2023

Front. Microbiol.

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IntroductionDengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1–4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent dengue and other vector-borne diseases.ObjectivesThe present study has been carried out to detect and characterize the circulating dengue virus (DENV) in field-collected Aedes mosquitoes in Bhopal, Central India.MethodsAedes mosquitoes were collected from 29 localities within Bhopal city during October 2020 to September 2022. DENV infection was assessed in the individual head and thorax regions of Aedes mosquitoes using reverse transcriptase PCR. Positive samples were sequenced, and the circulating serotypes and genotypes were determined using phylogenetic analysis.ResultsDENV RNA was detected in 7 Aedes aegypti and 1 Aedes albopictus, with infection rates of 0.59 and 0.14%, respectively. Phylogenetic analysis revealed all the isolates belonged to DENV serotype 2 and distinctly clustered with the non-Indian lineage (cosmopolitan genotype 4a), which was not recorded from the study area earlier. The time to most common recent ancestor (TMRCA) of these sequences was 7.4 years old, with the highest posterior density (HPD) of 3.5–12.2 years, indicating that this new lineage emerged during the year 2014. This is the first report on the DENV incrimination in both Ae. aegypti and Ae. albopictus mosquitoes collected from Bhopal, Central India.ConclusionThe observed emergence of the non-Indian lineage of DENV-2 in Bhopal, which again is a first report from the area, coincides with the gradual increase in DENV cases in Bhopal since 2014. This study emphasizes the importance of DENV surveillance and risk assessment in this strategically important part of the country to decipher its outbreak and severe disease-causing potential.

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Section: Discussionmentioning

confidence: 88%

“…All four serotypes of DENV have been linked to several outbreaks in India in the past ( Guo et al, 2017 ), however, the majority of these outbreaks were associated with DENV-2 ( Singh et al, 1999 ; Dash et al, 2006 ; Agarwal et al, 2023 ). The first DHF outbreak caused by DENV-2 occurred in New Delhi in 1996 ( Dar et al, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2

Sarma,

Rathod,

Mishra

et al. 2023

Front. Microbiol.

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Circulating serotypes and genotypes of dengue virus during the 2023 outbreak in Eastern Nepal

Bharati,

Dumre,

Shah

et al. 2024

Journal of Clinical Virology

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Dengue in Pune city, India (2017–2019): a comprehensive analysis

Arankalle,

Shrivastava,

Kulkarni

et al. 2024

Front. Public Health

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ObjectivesTo understand the dynamics of dengue disease with special reference to (1) age (2) primary/secondary infections (3) serostatus and (4) serotypes examined during three consecutive years.MethodsDuring 3 dengue seasons (2017–19), NS1/IgM ELISAs were used for dengue diagnosis in one of the 15 administrative wards of Pune City, India. Predefined symptoms were recorded at the time of diagnosis/hospitalization. IgG-capture ELISA (Panbio) was used to differentiate primary/secondary infections. DENV serotypes were determined for 260 viral RNA-positive patients.ResultsDuring the 3 years, 3,014/6,786 (44.4%, 41.4–49.9%) suspected cases were diagnosed as dengue. Use of either NS1 or IgM would have missed 25.5% or 43% of the confirmed dengue cases, respectively. Notably, a higher proportion of secondary dengue cases remained mild while a substantial proportion of primary infections developed warning signs. The symptoms among Dengue/non-dengue patients and primary/secondary infections varied and influenced by age and serostatus. The number and proportion of dengue serotypes varied yearly. A remarkable decline in dengue cases was observed during the COVID-19 pandemic years.ConclusionA substantial proportion of primary and secondary dengue patients progress to warning signs/severity or mild infection respectively, underscoring the possible role of non-ADE mechanisms in causing severe dengue that requires hospitalization. Both NS1 and IgM should be used for efficient diagnosis.

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Continued dominance of dengue virus serotype 2 during the recent Central India outbreaks (2019-2021) with evidence of genetic divergence

Cited by 4 publications

References 36 publications

Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2

Molecular surveillance of dengue virus in field-collected Aedes mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2

Circulating serotypes and genotypes of dengue virus during the 2023 outbreak in Eastern Nepal

Dengue in Pune city, India (2017–2019): a comprehensive analysis

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