Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming

Kollaran, Ameen M.; Joge, Shubham; Kotian, Harshitha S.; Badal, Divakar; Prakash, Deep; Mishra, Ayushi; Varma, Manoj M.; Singh, Varsha

doi:10.1016/j.isci.2019.02.028

Cited by 34 publications

(66 citation statements)

References 56 publications

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“…For example, FleR-FleS regulates flagellum biogenesis [63], and this TCS was found to be essential for biofilm formation on the ETT surface ( Fig. 3), consistent with another study of biofilm on polystyrene surface [40]. We postulated that these 112 TCS mutants with reduced biofilm might have defective flagella.…”

Section: Effect Of Tcs Mutations On Flagella-dependent Swimming Motilitysupporting

confidence: 87%

“…We noted certain similarities between TCS genes involved in the biofilm formation on ETTs (this study) and those reported for biofilm formation on other surfaces such as polystyrene and glass [40,72,73]. This included the regulator of flagellum biogenesis FleR-FleS, phosphate response regulator PhoQ, and AlgR-KinB (Fig.…”

Section: Discussionsupporting

confidence: 70%

“…Although a systematic analysis of the requirement of all TCS genes in biofilm formation has not been attempted in a single study, several studies have looked at the role of many TCS components in biofilm formation. The majority of these studies were done in microtitre plates (polystyrene material), and some on PVC surface or glass surface [11,16,25,40,73,[92][93][94][95][96][97][98][99][100]. These studies have reported many TCS mutants with increased biofilm biomass.…”

Section: Discussionmentioning

confidence: 99%

“…The two-component systems (TCSs), typically composed of a membrane-associated sensor kinase and an intracellular response regulator, endow bacteria with the ability to sense and adapt to external cues including metabolites, trace elements, antimicrobial peptides, pH and surfaces [39]. We have recently reported that 44 TCS encoding genes in P. aeruginosa PA14 regulate swarming behaviour in a media-dependent fashion, underscoring their role in sensing and responding to changing environmental cues [40]. One of the well-studied TCS is the GacA-GacS system in P. aeruginosa [41,42], which can regulate the secretion of Psl polysaccharide via RsmA [43].…”

Section: Introductionmentioning

confidence: 99%

“…Many studies on the biofilm of P. aeruginosa have focused on the biofilm formed on polystyrene or glass surface, flow cell or as colony biofilm on agar [16,40,52]. However, a systematic study of genetic regulators of biofilm formation on a clinically relevant surface such as endotracheal tubes or urinary catheter has not been performed.…”

Section: Introductionmentioning

confidence: 99%

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Pseudomonas aeruginosa biofilm formation on endotracheal tubes requires multiple two-component systems

Badal

Jayarani

Kollaran

et al. 2020

Journal of Medical Microbiology

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Introduction. Indwelling medical devices such as endotracheal tubes (ETTs), urinary catheters, vascular access devices, tracheostomies and feeding tubes are often associated with hospital-acquired infections. Bacterial biofilm formed on the ETTs in intubated patients is a significant risk factor associated with ventilator-associated pneumonia. Pseudomonas aeruginosa is one of the four frequently encountered bacteria responsible for causing pneumonia, and the biofilm formation on ETTs. However, understanding of biofilm formation on ETT and interventions to prevent biofilm remains lagging. The ability to sense and adapt to external cues contributes to their success. Thus, the biofilm formation is likely to be influenced by the two-component systems (TCSs) that are composed of a membrane-associated sensor kinase and an intracellular response regulator. Aim. This study aims to establish an in vitro method to analyse the P. aeruginosa biofilm formation on ETTs, and identify the TCSs that contribute to this process. Methodology. In total, 112 P. aeruginosa PA14 TCS mutants were tested for their ability to form biofilm on ETTs, their effect on quorum sensing (QS) and motility. Results. Out of 112 TCS mutants studied, 56 had altered biofilm biomass on ETTs. Although the biofilm formation on ETTs is QS-dependent, none of the 56 loci controlled quorum signal. Of these, 18 novel TCSs specific to ETT biofilm were identified, namely, AauS, AgtS, ColR, CopS, CprR, NasT, KdpD, ParS, PmrB, PprA, PvrS, RcsC, PA14_11120, PA14_32580, PA14_45880, PA14_49420, PA14_52240, PA14_70790. The set of 56 included the GacS network, TCS proteins involved in fimbriae synthesis, TCS proteins involved in antimicrobial peptide resistance, and surface-sensing. Additionally, several of the TCS-encoding genes involved in biofilm formation on ETTs were found to be linked to flagellum-dependent swimming motility. Conclusions. Our study established an in vitro method for studying P. aeruginosa biofilm formation on the ETT surfaces. We also identified novel ETT-specific TCSs that could serve as targets to prevent biofilm formation on indwelling devices frequently used in clinical settings.