Context-specific chromatin remodeling activity of mSWI/SNF complexes depends on the epigenetic landscape

Sokpor, Godwin; Nguyen, Huu Phuc; Tuoc, Tran

doi:10.1038/s41392-021-00770-6

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…The Brg1/Brm-associated factor (BAF) is the mammalian SWI/SNF complex which is a multi-subunit ATP-dependent chromatin remodeler. It is capable of re-structuring chromatin through histone mobilization and/or recruitment of epigenetic cofactors to modify the epigenetic landscape of chromatin ( Sokpor et al, 2021b ), leading to regulation of gene expression during brain development [reviewed in ( Sokpor et al, 2017 )]. We previously developed mouse models of inactive BAF complex.…”

Section: Introductionmentioning

confidence: 99%

BAF (mSWI/SNF) complex regulates mediolateral cortical patterning in the developing forebrain

Nguyen

Sokpor

Parichha

et al. 2022

Front. Cell Dev. Biol.

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Early forebrain patterning entails the correct regional designation of the neuroepithelium, and appropriate specification, generation, and distribution of neural cells during brain development. Specific signaling and transcription factors are known to tightly regulate patterning of the dorsal telencephalon to afford proper structural/functional cortical arealization and morphogenesis. Nevertheless, whether and how changes of the chromatin structure link to the transcriptional program(s) that control cortical patterning remains elusive. Here, we report that the BAF chromatin remodeling complex regulates the spatiotemporal patterning of the mouse dorsal telencephalon. To determine whether and how the BAF complex regulates cortical patterning, we conditionally deleted the BAF complex scaffolding subunits BAF155 and BAF170 in the mouse dorsal telencephalic neuroepithelium. Morphological and cellular changes in the BAF mutant forebrain were examined using immunohistochemistry and in situ hybridization. RNA sequencing, Co-immunoprecipitation, and mass spectrometry were used to investigate the molecular basis of BAF complex involvement in forebrain patterning. We found that conditional ablation of BAF complex in the dorsal telencephalon neuroepithelium caused expansion of the cortical hem and medial cortex beyond their developmental boundaries. Consequently, the hippocampal primordium is not specified, the mediolateral cortical patterning is compromised, and the cortical identity is disturbed in the absence of BAF complex. The BAF complex was found to interact with the cortical hem suppressor LHX2. The BAF complex suppresses cortical hem fate to permit proper forebrain patterning. We provide evidence that BAF complex modulates mediolateral cortical patterning possibly by interacting with the transcription factor LHX2 to drive the LHX2-dependent transcriptional program essential for dorsal telencephalon patterning. Our data suggest a putative mechanistic synergy between BAF chromatin remodeling complex and LHX2 in regulating forebrain patterning and ontogeny.

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Section: Introductionmentioning

confidence: 99%

BAF (mSWI/SNF) complex regulates mediolateral cortical patterning in the developing forebrain

Nguyen

Sokpor

Parichha

et al. 2022

Front. Cell Dev. Biol.

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“…(B) Typical m 6 A distribution in regions of an mRNA and its readout affects mRNA fates, including trafficking, stability, decay, translation, and localization. Based on: Zheng et al, 2020;Sokpor et al, 2021;modified. Rudy et al 10.3389/fpls.2022.1064131 Frontiers in Plant Science frontiersin.org…”

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confidence: 99%

N6-methyladenosine (m6A) RNA modification as a metabolic switch between plant cell survival and death in leaf senescence

et al. 2023

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Crop losses caused by climate change and various (a)biotic stressors negatively affect agriculture and crop production. Therefore, it is vital to develop a proper understanding of the complex response(s) to (a)biotic stresses and delineate them for each crop plant as a means to enable translational research. In plants, the improvement of crop quality by m6A editing is believed to be a promising strategy. As a reaction to environmental changes, m6A modification showed a high degree of sensitivity and complexity. We investigated differences in gene medleys between dark-induced leaf senescence (DILS) and developmental leaf senescence in barley, including inter alia RNA modifications active in DILS. The identified upregulated genes in DILS include RNA methyltransferases of different RNA types, embracing enzymes modifying mRNA, tRNA, and rRNA. We have defined a decisive moment in the DILS model which determines the point of no return, but the mechanism of its control is yet to be uncovered. This indicates the possibility of an unknown additional switch between cell survival and cell death. Discoveries of m6A RNA modification changes in certain RNA species in different stages of leaf senescence may uncover the role of such modifications in metabolic reprogramming. Nonetheless, there is no such data about the process of leaf senescence in plants. In this scope, the prospect of finding connections between the process of senescence and m6A modification of RNA in plants seems to be compelling.

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BRD7 as key factor in PBAF complex assembly and CD8+ T cell differentiation

Huang,

Lin,

Qiao

et al. 2024

JCI Insight

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Upon infection, naive CD8 + T cells differentiate into cytotoxic effector cells to eliminate the pathogen-infected cells. Although many mechanisms underlying this process have been demonstrated, the regulatory role of chromatin remodeling system in this process remains largely unknown. Here we show that BRD7, a component of the polybromo-associated BAF complex (PBAF), was required for naive CD8 + T cells to differentiate into functional short-lived effector cells (SLECs) in response to acute infections caused by influenza virus or lymphocytic choriomeningitis virus (LCMV). BRD7 deficiency in CD8 + T cells resulted in profound defects in effector population and functions, thereby impairing viral clearance and host recovery. Further mechanical studies indicate that the expression of BRD7 significantly turned to high from naive CD8 + T cells to effector cells, which bridged BRG1 and PBRM1 to the core module of PBAF complex, consequently facilitating the assembly of PBAF complex rather than BAF complex in the effector cells. The PBAF complex changed the chromatin accessibility at the loci of Tbx21 gene and upregulated its expression, leading to the maturation of effector T cells. Our research demonstrates that BRD7 and the PBAF complex are key in CD8 + T cell development and present a significant target for advancing immune therapies.

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Context-specific chromatin remodeling activity of mSWI/SNF complexes depends on the epigenetic landscape

Cited by 3 publications

References 5 publications

BAF (mSWI/SNF) complex regulates mediolateral cortical patterning in the developing forebrain

BAF (mSWI/SNF) complex regulates mediolateral cortical patterning in the developing forebrain

N6-methyladenosine (m6A) RNA modification as a metabolic switch between plant cell survival and death in leaf senescence

BRD7 as key factor in PBAF complex assembly and CD8+ T cell differentiation

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