Content of some flavonoids in alfalfa and its water extract "Eracond"

Abstract

The paper states that the extraction of some flavonoids from air-dry raw alfalfa with 70% alcohol-water solution was carried out. The quantitative determination of some flavonoids content in the obtained extracts and dietary supplement "Eracond" by HPLC method was carried out. The content of flavonoids in alfalfa ranges from 0.004 mg/g naringenin to 0.29 mg/g from dry basis mass in naringin, and it ranges from 0.007 mg/g of quercetin to 0.50 mg/g from dry mass in naringin in "Erakond". A higher level of quercetin (0.12 mg/g) was revealed in alfalfa harvested in June (first cut) relative to its content (0.08 ± 0.002) in alfalfa of the second cut. The content of dihydroquercetin (0.007 ± 0.0004 mg/g) and fisetin (0.04 mg/g) in the medicinal plant raw material harvested in August (L-2) decrease in relation to alfalfa harvested in June, where similar indicators were 0.088 ± 0.009 mg/g and 0.08 ± 0.003 mg/g, respectively. The phenolic compounds identified in alfalfa should be considered as promising pathogenetically substantiated agents in the complex therapy of pathologies involving the application of inhibitors of free radical processes.

Qualitative and quantitative analysis for the content of benzoic, salicylic, sorbic and glycyrrhizic acids in an aqueous extract of alfalfa

“…Результаты ранее проведённых собственных исследований показали, что БАД «Эраконд» содержит в своём составе широкий спектр полифенольных соединений, отличающихся высокой антиоксидантной активностью. Количественное определение полифенольных соединений в БАД «Эраконд» методом ВЭЖХ позволило идентифицировать и установить содержание следующих флавоноидов, мг/г «Эраконда»: нарингенин -0,05 ± 0,0006; рутин -8 ± 3; ликуразид -3,5 ± 0,4; кверцетин -0,04 ± 0,001; физетин -0,35 ± 0,01; нарингин -0,5 ± 0,0006; дигидрокверцетин -0,20 ± 0,002 [19].…”