Content and distribution of protein, sugars and inositol phosphates during the germination and seedling growth of two cultivars of Vicia faba

Goyoaga, Carmen; Burbano, Carmen; Cuadrado, Carmen; Romero, C.; Guillamón, Eva; Varela, Alejandro; Pedrosa, Mercedes M.; Múzquiz, M.

doi:10.1016/j.jfca.2010.11.002

Cited by 41 publications

(48 citation statements)

References 36 publications

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“…During germination of legume seeds their monosacharide, disaccharide and cyclitol contents increase (Goyoaga et al, 2011). In our experiments a positive correlation was found between seedling length and sugar contents.…”

Section: Two-dimensional Gel Electrophoresissupporting

confidence: 70%

Sparfloxacin ‒ A Potential Contaminant of Organically Grown Plants?

Ziółkowska¹,

Piotrowicz-Cieślak²

2016

Appl Ecol Env Res

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Abstract. Pharmaceuticals in water and soil ecosystems increasingly become a problem for environmental protection and agriculture. They may be transferred to soil and plants even in organic production systems as they often occur in manure. This paper presents assessment of sparfloxacin effect on protein profile and activity of guaiacol peroxidase in pea roots. The obtained results show that sparfloxacin modifies pea root proteome. In proteomic profiles of roots from seedlings grown at the highest sparfloxacin concentration (800 mg kg -1 ) there were 41 proteins more than in protein separations of roots of control seedlings, in which 176 proteins were identified. A higher number of proteins, compared to control roots (21 more), were also obtained on separations of root proteins from plants grown in substrate "contaminated" with 40 mg kg -1 sparfloxacin. With increasing sparfloxacin concentration the expression of root proteins involved in cell bioenergetics decreased, while isoflavone reductase, responsible for free radicle scavenging, became up-regulated. Moreover, the presence of sparfloxacin in soil resulted in increased activity of guaiacol peroxidase. The sparfloxacin content in roots was directly proportional to the level of this pharmaceutical in soil. At the highest concentration of sparfloxacin in soil (800 mg kg -1 ) the level of this compound in roots reached 247 µg g -1 fresh weight. Soluble carbohydrate contents in pea tissues correlated with seedling growth (root and stem elongation), which decreased parallel to increasing concentrations of soil sparfloxacin.

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Section: Two-dimensional Gel Electrophoresissupporting

confidence: 70%

Sparfloxacin ‒ A Potential Contaminant of Organically Grown Plants?

Ziółkowska¹,

Piotrowicz-Cieślak²

2016

Appl Ecol Env Res

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“…The initial increase in sugars in the cotyledons may be an accumulation of sucrose, and since these are not readily mobilized, they therefore accumulate, as observed in Pisum sativum seeds (Monerri et al, 1986). Increases in sucrose concentrations were observed in the cotyledons of Vicia faba seeds until the second day after the start of imbibition, but decreasing significantly after this until day seven (Goyoaga et al, 2011).…”

Section: Resultsmentioning

confidence: 87%

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

et al. 2013

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Seed imbibitions is the first stage of the germination process and is characterized by the hydration of tissues and cells and the activation and/or induction of the enzymes responsible for mobilizing reserves for respiration and the construction of new cell structures. The objective of this study was to investigate the alterations in reserve substances during slow hydration of Bahia Rosewood (Dalbergia nigra) seeds in water. Seeds from two different lots (Lot I and II) were placed in saturated desiccators (95-99% RH) to hydrate at 15 and 25 °C until water contents of 10, 15, 20 and 25% were reached. At each level of hydration, changes in lipid reserves, soluble carbohydrates, starch and soluble proteins were evaluated. The mobilization of reserves was similarly assessed in both lots, with no differences being observed between the two hydration temperatures. Lipid contents showed little variation during hydration, while the contents of soluble carbohydrates and starch decreased after the 15% water content level. Soluble proteins showed a gradual tendency to decrease between the control (dry seeds) up to 25% water content.

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“…A faixa de temperatura entre 20 e 30 ºC tem sido indicada para a germinação de inúmeras espécies florestais tropicais e subtropicais, dentre as quais Peltogyne paniculata (Ramos et al, 2007), Adenanthera pavonina (Kissmann et al, 2008), Senna alata (Braga et al, 2010), Tabebuia ochracea (Oliveira et al, 2012), Myracroduon urundeuva (Virgens et al, 2012) e Apeiba tibourbou (Guedes et al, 2013 Durante a germinação, os lipídios são hidrolisados e convertidos a sacarose pela β-oxidação e ciclo do glioxalato, para fornecerem energia e esqueletos de carbono para a plântula em formação (Theoudoulou & Eastmond, 2012 Os carboidratos pré-formados nas sementes servem como substrato durante o período pré-germinativo (Bewley et al, 2013), sendo os oligossacarídeos da série rafinósica utilizados como fontes primárias de energia em sementes de Dalbergia nigra e Vicia faba, respectivamente (Carrijo et al, 2010;Goyoaga et al, 2011). Na hidratação das sementes iniciase o gasto de energia, suprida pelas reservas de sacarose, possibilitando às células os níveis de atividade metabólica necessários para sustentar o desenvolvimento do embrião.…”

Section: Resultsunclassified

Alterações nas reservas de sementes de Melanoxylon brauna Schott. (Fabaceae Caesalpinoideae) durante a germinação em diferentes temperaturas

Ataíde

Borges

Picoli

et al. 2017

Agraria

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RESUMODurante a germinação, as sementes utilizam os compostos de reserva para fornecerem os nutrientes necessários até que as plântulas se tornem autotróficas. Neste trabalho objetivou-se investigar as alterações nas reservas de carboidratos, amido, lipídios e proteínas em sementes de Melanoxylon brauna durante o período germinativo em diferentes temperaturas. Para tanto, sementes da espécie foram colocadas para germinar sob temperaturas de 10, 25, 30 e 40 °C, sendo retiradas amostras de sementes a cada 24 horas durante o período de 96 horas. Uma pequena redução nos teores de lipídios foi observada durante a germinação nas temperaturas analisadas, enquanto os teores de açúcares solúveis decresceram a partir de 48 horas de embebição, sendo prontamente utilizados, especialmente nas temperaturas de 25 e 30 °C. Para as proteínas solúveis, verificou-se declínio desde o início do período germinativo às temperaturas de 25 e 30 °C, sendo este mais intenso no período pós-germinativo à temperatura de 30 °C. Conclui-se que as reservas de carboidratos solúveis, lipídios e proteínas decresceram durante o período germinativo de sementes de Melanoxylon brauna, variando esses decréscimos de acordo com a temperatura. Palavras-chave: amido; carboidratos; lipídios; proteínas Changes in seed reserves of Melanoxylon brauna Schott. (Fabaceae Caesalpinoideae) during germination at different temperatures ABSTRACTDuring germination, seeds use the reserve compounds to provide the nutrients until the seedlings become autotrophic. The aim of this study was to investigate the changes in carbohydrates, starch, lipids and proteins reserves during germination of Melanoxylon brauna seeds at different temperatures. For both, the seeds were germinated at temperatures of 10, 25, 30 and 40 °C, and samples were collected every 24 hours during 96 hours. Was observed small reduction of lipid levels during germination in temperatures analyzed, while the levels of soluble sugars decreased from 48 hours of soaking, being readily used, especially at temperatures of 25 and 30 °C. For soluble proteins, there was a decrease from the beginning of the germination period at temperatures of 25 to 30 °C, this being more intense in the post-germination at 30 °C period. It is concluded that soluble carbohydrate, lipid and protein reserves decreased during the germination period of Melanoxylon brauna seeds, varying these decreases according to temperature.

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Content and distribution of protein, sugars and inositol phosphates during the germination and seedling growth of two cultivars of Vicia faba

Cited by 41 publications

References 36 publications

Sparfloxacin ‒ A Potential Contaminant of Organically Grown Plants?

Sparfloxacin ‒ A Potential Contaminant of Organically Grown Plants?

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

Alterações nas reservas de sementes de Melanoxylon brauna Schott. (Fabaceae Caesalpinoideae) durante a germinação em diferentes temperaturas

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