Contemporary proteomic research on lignocellulosic enzymes and enzymolysis: A review

Guo, Hongliang; He, Tongyuan; Lee, Dong Hoon

doi:10.1016/j.biortech.2021.126263

Cited by 19 publications

(4 citation statements)

References 127 publications

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“…Phytopathogenic microorganisms such as bacteria and fungi produce an arsenal of different enzymatic activities which, when combined, can degrade all lignocellulosic and protein components of a plant cell wall in order to invade and infect the host plant [ 107 , 108 , 109 , 110 , 111 , 112 ]. In that aspect, parasitic plants, during, host invasion, are capable of degrading lignocellulosic components and protein through the synthesis of cellulases, xylanases, glucanases, pectinesterases, and peptidases [ 1 , 113 , 114 , 115 ].…”

Section: Discussionmentioning

confidence: 99%

Protein Profiling of Psittacanthus calyculatus during Mesquite Infection

Aguilar-Venegas

Quintana-Rodríguez²,

Aguilar-Hernández³

et al. 2023

Plants

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Psittacanthus calyculatus is a hemiparasite mistletoe that represents an ecological problem due to the impacts caused to various tree species of ecological and commercial interest. Although the life cycle for the Psittacanthus genus is well established in the literature, the development stages and molecular mechanism implicated in P. calyculatus host infection are poorly understood. In this study, we used a manageable infestation of P. laevigata with P. calyculatus to clearly trace the infection, which allowed us to describe five phenological infective stages of mistletoe on host tree branches: mature seed (T1), holdfast formation (T2), haustorium activation (T3), haustorium penetration (T4), and haustorium connection (T5) with the host tree. Proteomic analyses revealed proteins with a different accumulation and cellular processes in infective stages. Activities of the cell wall-degrading enzymes cellulase and β-1,4-glucosidase were primarily active in haustorium development (T3), while xylanase, endo-glucanase, and peptidase were highly active in the haustorium penetration and xylem connection (T4). Patterns of auxins and cytokinin showed spatial concentrations in infective stages and moreover were involved in haustorium development. These results are the first evidence of proteins, cell wall-degrading enzymes, and phytohormones that are involved in early infection for the Psittacanthus genus, and thus represent a general infection mechanism for other mistletoe species. These results could help to understand the molecular dialogue in the establishment of P. calyculatus parasitism.

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Section: Discussionmentioning

confidence: 99%

Protein Profiling of Psittacanthus calyculatus during Mesquite Infection

Aguilar-Venegas

Quintana-Rodríguez²,

Aguilar-Hernández³

et al. 2023

Plants

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“…The main components of most of these biomasses are cellulose, hemicellulose, and lignin ( Zerva et al, 2021 ). Naturally, a wide range of microorganisms carry a rich and sophisticated enzymatic arsenal, including cellulase, hemicellulase, ligninase, and auxiliary enzymes, which could completely degrade waste biomass into fermentable sugars ( Guo H. et al, 2022 ; Guo X. et al, 2022 ). These hydrolytic enzymes have been extensively studied ( Guo et al, 2023 ), such as cellulase in Bacillus tequilensis (ON754229) ( Malik and Javed, 2024 ), hydrolase in cellulolytic nitrogen-fixing bacteria ( Harindintwali et al, 2022 ), and β-glucosidase and xylanase in Trichoderma asperellum LYS1 ( Mou et al, 2023 ).…”

Section: Production Of Sa From Waste Biomassmentioning

confidence: 99%

Advances in succinic acid production: the enhancement of CO2 fixation for the carbon sequestration benefits

Lin,

Li,

Wang

et al. 2024

Front. Bioeng. Biotechnol.

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Succinic acid (SA), one of the 12 top platform chemicals produced from biomass, is a precursor of various high value-added derivatives. Specially, 1 mol CO2 is assimilated in 1 mol SA biosynthetic route under anaerobic conditions, which helps to achieve carbon reduction goals. In this review, methods for enhanced CO2 fixation in SA production and utilization of waste biomass for SA production are reviewed. Bioelectrochemical and bioreactor coupling systems constructed with off-gas reutilization to capture CO2 more efficiently were highlighted. In addition, the techno-economic analysis and carbon sequestration benefits for the synthesis of bio-based SA from CO2 and waste biomass are analyzed. Finally, a droplet microfluidics-based high-throughput screening technique applied to the future bioproduction of SA is proposed as a promising approach.

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“…These enzymes are mainly composed of cellulases, hemicellulases, ligninases, and auxiliary enzymes responsible for the degradation of the plant cell wall constituents [ 15 ]. So far, many lignocellulolytic enzymes produced by microorganisms, have been identified and clustered into various families in the Carbohydrate-Active EnZyme database (CAZyme), taking their sequence and structural-functional characteristics as a basis for their classification [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Lignocellulolytic Enzymes Production by Four Wild Filamentous Fungi for Olive Stones Valorization: Comparing Three Fermentation Regimens

Arif,

M’Barek,

Bekaert

et al. 2024

J. Microbiol. Biotechnol.

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Lignocellulolytic enzymes play a crucial role in efficiently converting lignocellulose into valuable platform molecules in various industries. However, they are limited by their production yields, costs, and stability. Consequently, their production by producers adapted to local environments and the choice of low-cost raw materials can address these limitations. Due to the large amounts of olive stones (OS) generated in Morocco which are still undervalued, Penicillium crustosum, Fusarium nygamai, Trichoderma capillare, and Aspergillus calidoustus, are cultivated under different fermentation techniques using this by-product as a local lignocellulosic substrate. Based on a multilevel factorial design, their potential to produce lignocellulolytic enzymes during 15 days of dark incubation was evaluated. The results revealed that P. crustosum expressed a maximum total cellulase activity of 10.9 IU/ml under sequential fermentation (SF) and 3.6 IU/ml of β-glucosidase activity under submerged fermentation (SmF). F. nygamai recorded the best laccase activity of 9 IU/ml under solid-state fermentation (SSF). Unlike T. capillare, SF was the inducive culture for the former activity with 7.6 IU/ml. A. calidoustus produced, respectively, 1,009 μg/ml of proteins and 11.5 IU/ml of endoglucanase activity as the best results achieved. Optimum cellulase production took place after the 5th day under SF, while ligninases occurred between the 9th and the 11th days under SSF. This study reports for the first time the lignocellulolytic activities of F. nygamai and A. calidoustus. Furthermore, it underlines the potential of the four fungi as biomass decomposers for environmentally-friendly applications, emphasizing the efficiency of OS as an inducing substrate for enzyme production.

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Contemporary proteomic research on lignocellulosic enzymes and enzymolysis: A review

Cited by 19 publications

References 127 publications

Protein Profiling of Psittacanthus calyculatus during Mesquite Infection

Protein Profiling of Psittacanthus calyculatus during Mesquite Infection

Advances in succinic acid production: the enhancement of CO2 fixation for the carbon sequestration benefits

Lignocellulolytic Enzymes Production by Four Wild Filamentous Fungi for Olive Stones Valorization: Comparing Three Fermentation Regimens

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