Contemporary glycomic approaches using ion mobility–mass spectrometry

Morrison, Kelsey A.; Clowers, Brian H.

doi:10.1016/j.cbpa.2017.11.020

Cited by 37 publications

(23 citation statements)

References 76 publications

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“…[3][4][5] Consequently, MS is often combined with other techniques for glycan analysis. 6,7 One such technique, vibrational spectroscopy, has begun to show promise for identifying glycan isomers, owing to its extremely high sensitivity to specific hydrogen-bonding patterns. 8,9 Polfer and coworkers first used infrared multiphoton dissociation (IRMPD) to measure vibrational spectra of gas-phase monosaccharides, 10,11 and Schindler et al extended this approach to determine glycosidic bond anomericity of small glycans.…”

Section: Introductionmentioning

confidence: 99%

Cryogenic Ion Spectroscopy for Identification of Monosaccharide Anomers

Scutelnic¹,

Rizzo²

2019

J. Phys. Chem. A

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We combine conformer-selective, cryogenic infrared spectroscopy, quantum mechanical computations, and 18 O substitution at the reducing end to determine the structural preferences of protonated glucosamine in the gas phase. Cryogenic infrared-infrared (IR-IR) double resonance spectroscopy of helium-tagged protonated glucosamine provides vibrational fingerprints of individual conformers, and 18 O isotopic labeling facilitates the match with computed structures and provides a selective probe of the anomeric hydroxyl. This is key for using vibrational spectroscopy for glycan analysis and determining the generality of anomeric memory during glycosidic bond cleavage.

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Section: Introductionmentioning

confidence: 99%

Cryogenic Ion Spectroscopy for Identification of Monosaccharide Anomers

Scutelnic¹,

Rizzo²

2019

J. Phys. Chem. A

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“…7 Another emerging and promising technique capable of separating glycan isomers is ion mobility spectrometry (IMS). 5,8,9 Here, ions travel through a drift cell filled with an inert buffer gas under the influence of a weak electric field and undergo low-energy collisions with the buffer gas. Compact ions collide less frequently with the buffer gas than more extended ions, which leads to a separation based on size, shape and charge.…”

Section: Introductionmentioning

confidence: 99%

Separation of isomeric glycans by ion mobility spectrometry – the impact of fluorescent labelling

Manz

Grabarics

Hoberg

et al. 2019

Analyst

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“…Ion mobility-mass spectrometry (IM-MS) is also a promising analytical tool in this field with the added value of being able to separate analytes based on their size. This technique has been successfully applied for the separation and characterization of isobaric polysaccharides and glycopeptides [100,101].…”

Section: O/n Glycosylationmentioning

confidence: 99%

Current Trends in the Analysis of Post-translational Modifications

et al. 2019

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Post-translational modifications controlling a large number of biological functions are key aspects of protein diversity. They have an important role controlling cellular processes and may be advantageously utilized. Qualitative and quantitative analyses of post-translational modifications are useful for biomarker research and an integral part of the characterization of protein biopharmaceuticals. Due to its sensitivity and widespread applicability, mass spectrometry has become the core technology of the analysis especially when combined with chromatographic and other separation techniques. The aim of this article is to present a general overview of mass spectrometry applications in the field of PTM mapping. We also present the analytical challenges of particular PTMs, primarily focusing on the most frequent modifications.

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Contemporary glycomic approaches using ion mobility–mass spectrometry

Cited by 37 publications

References 76 publications

Cryogenic Ion Spectroscopy for Identification of Monosaccharide Anomers

Cryogenic Ion Spectroscopy for Identification of Monosaccharide Anomers

Separation of isomeric glycans by ion mobility spectrometry – the impact of fluorescent labelling

Current Trends in the Analysis of Post-translational Modifications

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