Contaminations in mass cultivation of cyanobacteria: Highly resilient Colpoda steinii leads to rapid crash of Synechocystis sp. cultures and is inhibited by partially anoxic conditions

Troschl, Clemens; Fritz, Ines; Sodnikar, Katharina; Drosg, B.

doi:10.1016/j.algal.2017.11.002

Cited by 22 publications

(16 citation statements)

References 26 publications

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“…The initial response of the culture strongly resembles the description of culture crash occurrence found by Troschl et al (2017) in cultures of cyanobacteria Synechocystis sp., which was caused by contamination by the ciliate Colpoda steinii . Ciliates are protozoa which feed on smaller organisms, such as bacteria and algae, and can rapidly wipe out algae cultures.…”

Section: Resultssupporting

confidence: 80%

“…Contaminants are one of the major drawbacks for cultivation at industrial scale, not only in open race‐way ponds, but also in closed photobioreactors, as the scale of operations can limit the possibilities for sterile cultivation, both from operational and economic perspectives. Having a strain which can resist to grazing is beneficial to the process, as it can reduce the requirement for alternative strategies to attempt to deal with contaminants, such as increase salinities, pH values, and/or CO 2 concentrations (Troschl et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

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Outdoor scale‐up of Leptolyngbya sp.: Effect of light intensity and inoculum volume on photoinhibition and ‐oxidation

Schipper

Das

Muraikhi

et al. 2021

Biotech & Bioengineering

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The effect of light intensity and inoculum volume on the occurrence of photooxidation for Leptolyngbya sp. QUCCCM 56 was investigated, to facilitate the transition from small-scale laboratory experiments to large-scale outdoor cultivation. Indoor, the strain was capable of growing at light intensities of up to 5600 µmol photons/m 2 /s, at inoculation densities as low as 0.1 g/L (10% inoculation volume vol/vol). Levels of chlorophyll and phycocyanin showed a significant decrease within the first 24 h, indicating some level of photooxidation, however, both were able to recover within 72 h. When cultivated under outdoor conditions in Qatar during summer, with average peak light intensities 1981 ± 41 μmol photons/m 2 /s, the strain had difficulties growing. The culture recovered after an initial adaptation period, and clear morphological differences were observed, such as an increase in trichome length, as well as coiling of multiple trichomes in tightly packed strands. It was hypothesized that the morphological changes were induced by UV-radiation as an adaptation mechanism for increased self-shading. Furthermore, the presence of contaminating ciliates could have also affected the outdoor culture. Both UV and contaminants are generally not simulated under laboratory environments, causing a mismatch between indoor optimizations and outdoor realizations.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Outdoor scale‐up of Leptolyngbya sp.: Effect of light intensity and inoculum volume on photoinhibition and ‐oxidation

Schipper

Das

Muraikhi

et al. 2021

Biotech & Bioengineering

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“…We previously described the necessary contamination control for the non-sterile cultivation of Synechocystis sp. [26]. Stable cultivation of this small cyanobacterium was only possible with partially anoxic conditions.…”

Section: Description Of the Culture And Contamination Monitoringmentioning

confidence: 99%

Pilot-scale production of poly-β-hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactor

et al. 2018

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Poly-β-hydroxybutyrate (PHB) is considered as one of the most promising bioplastics. It has a broad range of applications and is degraded relatively rapidly by soil organisms. Like many prokaryotes, the cyanobacterium Synechocystis sp. CCALA192 produces this biopolymer as a storage compound, especially under nutrient starvation.In a 200-liter tubular photobioreactor, we cultivated Synechocystis sp. CCALA192 semi-continuously over a period of 75 days with CO 2 as sole carbon source. A two-stage cultivation strategy was performed, where after 5-7 days nitrogen was depleted and the culture started to produce PHB and gradually turned from blue-green to yellow. After 16-20 days, 90% of the culture was harvested and the residual 10% was used as inoculum for the following cycle. The harvested culture had an average biomass concentration of 1.0 g/L with an average PHB content of 12.5% of cell dry weight. After restarting with fresh nutrients, the yellow culture turned blue-green again and degraded the PHB within 24-48 hours. When nitrogen of the medium was consumed, PHB was produced again and the cycle continued. In the late stage of each production cycle, a ripening process was observed, where no CO 2 was consumed but the PHB concentration was still rising at the expense of the existing glycogen rich biomass.Establishing a stable Synechocystis sp. CCALA192 culture under non-sterile conditions turned out to be difficult, as this small unicellular organism is very sensitive and easily grazed by protozoa. Therefore, a special cultivation strategy with partially anoxic conditions was necessary.

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“…Colpoda steinii , for instance, can diminish a dense culture of the cyanobacterium Synechocystis sp. in a relatively short time [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Techniques to Control Microbial Contaminants in Nonsterile Microalgae Cultivation

Pleißner

Lindner

Ambati

2020

Appl Biochem Biotechnol

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The aim of this mini-review with own results was an identification of techniques to suppress the growth of microbial contaminants under photo- and mixotrophic conditions. Techniques identified are the modification of environmental conditions, such as pH, oxygen, and nutrient concentrations, as well as the application of pulsed electric field, ultrasonication, and surfactants. In phototrophic cultivations, the mentioned techniques result in a decrease of number of predatory cells, but not in a complete removal. Measures to suppress the growth of contaminations (e.g., bacteria and fungi) in mixotrophic cultivations could not be identified. The co-cultivation of algae and fungi, however, was found to be beneficial for the utilization of unusual carbon compounds (e.g., phenolic compounds).

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Contaminations in mass cultivation of cyanobacteria: Highly resilient Colpoda steinii leads to rapid crash of Synechocystis sp. cultures and is inhibited by partially anoxic conditions

Cited by 22 publications

References 26 publications

Outdoor scale‐up of Leptolyngbya sp.: Effect of light intensity and inoculum volume on photoinhibition and ‐oxidation

Outdoor scale‐up of Leptolyngbya sp.: Effect of light intensity and inoculum volume on photoinhibition and ‐oxidation

Pilot-scale production of poly-β-hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactor

Techniques to Control Microbial Contaminants in Nonsterile Microalgae Cultivation

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