Contamination of Primary Cultures in Tropical Areas: The Case of Hevea Brasiliensis

Enjalric, Frank; Carron, Marc-Philippe; Lardet, Ludovic

doi:10.17660/actahortic.1988.225.6

Cited by 40 publications

(25 citation statements)

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“…There are several pathogens (microbial contaminants) which have been a major threat to in vitro cultures due to their rapid proliferation characteristics (Enjalric et al, 1998). Axenic cultures are often mandatory throughout the entire in vitro culture process.…”

Section: In Vitro Culture Pathogensmentioning

confidence: 99%

Efficacy and Utilization of Fungicides and Other Antibiotics for Aseptic Plant Cultures

Mng’omba¹,

Sileshi²,

Toit³

et al. 2012

Fungicides for Plant and Animal Diseases

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Section: In Vitro Culture Pathogensmentioning

confidence: 99%

Efficacy and Utilization of Fungicides and Other Antibiotics for Aseptic Plant Cultures

Mng’omba¹,

Sileshi²,

Toit³

et al. 2012

Fungicides for Plant and Animal Diseases

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“…The unsatisfactory yield of sterile, actively growing shoots of fruit trees in-vitro using conventional methods of surface sterilisation has been documented by several workers (Enjalric et al 1988;Hennerty et al 1988;James & Thurbon 1978;Laimer et al 1988;Webster & Jones 1989). Protocols for surface disinfestation that consistently yield a high number of uncontaminated explants would be highly desirable (Hennerty et al 1988).…”

Section: Discussionmentioning

confidence: 99%

“…Keeping the donor plants under glasshouse conditions and pretreating them with antimicrobial substances allows considerable improvement of their phytosanitary state and decreases the infection pressure (Debergh 1987;Duhem et al 1988;Enjalric et al 1988). Keeping the donor plants under glasshouse conditions and pretreating them with antimicrobial substances allows considerable improvement of their phytosanitary state and decreases the infection pressure (Debergh 1987;Duhem et al 1988;Enjalric et al 1988).…”

Section: Introductionmentioning

confidence: 99%

A new, efficient method using 8-hydroxy-quinolinol-sulfate for the initiation and establishment of tissue cultures of apple from adult material

Machado¹,

Machado²,

Hanzer³

et al. 1991

Plant Cell Tiss Organ Cult

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Applying the new method for culture initiation 16 different cultivars of Malus domestica could be established in vitro from shoot tips of adult orchard trees. Actively growing shoot tips were cleaned and surface disinfested, dissected to 2-3 mm and placed on a modified MS-medium with 4.4/xM BA. Explants were covered for 24 h with 200/zl of a 0.1% solution of 8-hydroxy-quinolinol-sulfate (8-HQS) and transferred to a medium containing both auxin and cytokinin after 2 weeks. The application of 8-HQS induced a strong reduction of the infection rate and inhibited the browning of the explants and the media. After 7 days yields of 50-90% sterile explants could be obtained in comparison to 100% losses of untreated shoot tips. After 60 days variable rates of actively growing shoots could be observed, depending on the genotypes. The described method allows a successful establishment of fruit trees from adult orchard material on one hand by strongly reducing the browning, caused by the oxidation of polyphenolic compounds by polyphenoloxidases, and on the other hand 8-HQS can strongly increase the yield of explants without contamination, independently from the vegetation period and the phytosanitary state of the donor material.Abbreviations: BA-6-benzyladenine, IBA-indolebutyric acid, 8-HQS-8-hydroxy-quinolinol-sulfate

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“…Endophytes may be intercellular pathogens, as well as opportunistic and adapted colonizers originating from the environment; or intracellular viruses, viroids, and bacteria. Several plants and related environmental factors such as plant species, age, explant source and prevailing weather condition contribute to in vitro cultures [8]. Despite the best timing and selection efforts it is almost impossible to eliminate contamination from in vitro grown plants.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of MDR Bacteria From in Vitro Culture of Bacopa Monniera and Supplementation of Natural Extracts to Control Bacterial Contamination

Sharma

Bhardwaj

Nagar³

et al. 2016

Int J Pharm Pharm Sci

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Objective: The purpose of the present study was to isolate and characterize bacterial contamination from in vitro culture of Bacopa monniera callus culture. Methods:The two selected isolates (1 and 2) were identified by morphological, biochemical and molecular (16S rRNA gene sequencing) methods. Beside this antibiotic resistance was also determined. Results:The isolates were identified as closely related to Enterobacter cloacae (KU350623) (Isolate 1) and Myroides odoratimimus ( Conclusion:The present studies suggest using natural extracts supplementation as a promising strategy to control the in vitro bacterial contamination in plant tissue culture. KU382740) (Isolate 2).The isolate 1 and 2 were found to be resistant to streptomycin (25 mcg), dapsone (10 mcg), erythromycin (15 mcg), chloroamphenicol (25 mcg) and ampicillin (10 mcg). Supplementation of the natural extract was tested to control the contamination due to these multi drug resistant bacteria. Aqueous and alcoholic leaf extracts of Azadirachta indica was added to plant tissue culture media i.e. MS media in order to control contamination.

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Contamination of Primary Cultures in Tropical Areas: The Case of Hevea Brasiliensis

Cited by 40 publications

References 0 publications

Efficacy and Utilization of Fungicides and Other Antibiotics for Aseptic Plant Cultures

Efficacy and Utilization of Fungicides and Other Antibiotics for Aseptic Plant Cultures

A new, efficient method using 8-hydroxy-quinolinol-sulfate for the initiation and establishment of tissue cultures of apple from adult material

Isolation and Characterization of MDR Bacteria From in Vitro Culture of Bacopa Monniera and Supplementation of Natural Extracts to Control Bacterial Contamination

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