Contamination of mononuclear cell suspensions obtained from cancer patients by the Böyum method

Response of human lymphocytes to PHA and tumour-associated antigens as detected by fluorescence polarization

“…From our results it must be noted that the number of contaminating granulocytes in the "lymphocyte" cell suspension appears to affect the change in P after PHA incubation. This may be important, since the proportion of granulocytes isolated from non-carbonyliron-treated blood samples by the density gradient method has been found to be higher and to include some imnmature forms, in cancer patients, compared with healthy controls (Currie et al, 1978). As can be seen from Tables I and III, not all granulocytes are removed by treatment of the blood sample with carbonyl iron.…”

Granulocyte contamination of separated blood mononuclear cells from spontaneously tumorous mice

“…Firstly, they provide support for the contention that Ficoll-Hypaque-separated "peripheral blood mononuclear cells" from cancer patients may have marked immaturegranulocyte contamination which might affect the validity of published reports of functional lymphocyte abnormalities (Currie et al, 1978). Since the separation method is density-dependent, this contamination is unavoidable if the cells are isopycnic.…”

“…However, in certain circumstances, this assumption may lead to dangerous misinterpretation. Currie et al (1978) recently demonstrated that Ficoll-Hypaque-separated peripheralblood mononuclear cells from human patients with advanced malignant disease were frequently contaminated with immature granulocytes. In a substantial number of their patients, "left-shifted" granulocytes were so numerous that lymphocytes were reduced to a minor subpopulation of the separated cells.…”

Antibody-dependent cellular cytotoxicity to human colon-tumour cells. I. Lack of tumour specificity in a population study

“…The works of Lovehik & Hong (1977), Elhilali et al (1976 and Peter et al (1975a) support, our' observations, wrhereas Ting & Terasaki (1974) found (lepressed ADCC-effector activity in cancer patients. It, has to be emphasized that the Ficolle-Hypaqueseparated mononuclear cell preparations may contain substantial numbers of macrophages (Zucker-Franklin, 1974) and chloroacetate-esterase-positive cells and that the percentage of the last-mentionied cells may be especially high in cancer patients (Currie et al, 1978). Although such cells may participate in ADCC (Kohl et al, 1977;GXale & Zighelboim, 1975) (Shoham & Cohen, 1979), which brings evidence that the reaction observed is to an antigenic determinant common to CEA and A, and maybe normal colon antigen too.…”

“…Such cell samples contained 54-7 + 10-2% and 50 7 + 117% E rosettes in normal adults and cancer patients respectively (Shoham et al, to be published), and 5-10% B rosettes (with mouse red blood cells). The "null" cell population was not further analysed for the presence of non-lymphocyte white blood cells (ZuckerFranklin, 1974;Currie et al, 1978). Nevertheless, we will refer conventionally to these mononuclear cell preparations as peripheralblood lymphocytes (PBL) with the understanding that other white blood cells present in these preparations may contribute to the ADCC reaction (Kohl et al, 1977;Gale & Zighelboim, 1975 (Tables I and II).…”