Contamination of honey produced in the Republic of Kazakhstan with Clostridium botulinum

Mustafina, Raikhan; Maikanov, Balgabay; Wisniewski, J.; Tracz, Michał; Anusz, Krzysztof; Grenda, Tomasz; Kukier, E.; Goldsztejn, Magdalena; Kwiatek, K.

doi:10.1515/bvip-2015-0036

Cited by 10 publications

(11 citation statements)

References 19 publications

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“…Midura ( 15 ) reported the contamination of 10% (9/90) of examined samples in the USA. However, in Khazakhstan, Mustafina et al ( 16 ) noticed the occurrence of C. botulinum only in 0.91% (1/120) of honey samples.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of C. botulinum and C. perfringens spores in food products available on Polish market

Grenda

Grabczak

Kwiatek

et al. 2017

Journal of Veterinary Research

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IntroductionThe aim of this study was to evaluate the prevalence of Clostridium botulinum and Clostridium perfringens in food samples purchased from Polish producers.Material and MethodsThe analyses were performed on 260 food samples collected in Lublin and Subcarpathian regions: 56 of smoked meat, 21 of pork meat, 20 of dairy products, 26 of vegetable and fruit preserves, 40 of ready-to-eat meals, 27 of fish preserves, and 70 of honey collected directly from apiaries.ResultsC. botulinum strains were isolated from 2.3% (6/260) of samples and the isolates were classified as toxin types A (4/260) and B (2/260). C. perfringens strains were isolated from 14% (37/260) of samples. All the isolates were classified as toxin type A, 28 of them were able also to produce α toxin and 9 – β2 toxin.ConclusionOn the basis of the obtained results it could be suggested that risk assessment, especially regarding the entire honey harvesting process, should be provided in order to ensure the microbiological safety of the products to be consumed by infants and people with a weakened immune system.

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Section: Discussionmentioning

confidence: 99%

Prevalence of C. botulinum and C. perfringens spores in food products available on Polish market

Grenda

Grabczak

Kwiatek

et al. 2017

Journal of Veterinary Research

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“…Since 1976, there have been over 1,500 cases of infant botulism reported in more than 15 countries worldwide [ 16 ]. Among the various potential sources of C. botulinum spores (soil, dust, etc.…”

Section: Discussionmentioning

confidence: 99%

“…Midura [ 15 ] reported C. botulinum occurrence in 10.0% (9/90) of examined samples from the USA. In Kazakhstan, Mustafina et al [ 16 ] noted C. botulinum occurrence in only 0.9% (1/110) of honey samples. Polish honey was examined by two independent scientific teams and a sample contamination level of 8.6% (6/70) from the Lublin and Subcarpathia provinces of Poland was described by Grenda et al [ 8 ], whereas Wojtacka et al [ 27 ] detected C. botulinum spores in 21.6% (22/102) of samples from small apiaries in an undetermined area in Poland.…”

Section: Discussionmentioning

confidence: 99%

Clostridium botulinumspores in Polish honey samples

et al. 2018

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The aim of this study was an examination of 240 multifloral honey samples collected from Polish apiaries to determine Clostridium botulinum occurrence. Honey was collected from apiaries directly after the extraction process. Samples were inoculated by using the dilution and centrifugation method. Suspected isolates were examined by using mouse bioassay, polymerase chain reaction (PCR), and real-time PCR methods. C. botulinum type A and B strains were detected in 5 of 240 examined honey samples (2.1%). Bacterial strains were also detected that were phenotypically similar to C. botulinum but that did not exhibit the ability to produce botulinum toxins and did not show the presence of the botulinum cluster (ntnh and bont genes) or expression of the ntnh gene. The methods used in the examination, especially the expression analysis of ntnh gene, enabled specific analysis of suspected strains and could be used routinely in environmental isolate analyses of C. botulinum occurrence.

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“…Contamination with Clostridia spores was reported by Mustafina et al . (8) who noticed their presence in 39.1% (43/110) of examined samples collected in Kazakhstan. Różańska and Osek (14) observed that honey samples from Poland were contaminated by Clostridia spores at a mean level of 22.9% (25/109).…”

Section: Discussionmentioning

confidence: 99%

“…are bacteria able to produce spores resistant to unfavourable conditions and may survive despite the high concentration of sugars and occurrence of antibacterial substances. The hygienic and epidemiological aspects of infection by this genus, and especially its pathogenic species like C. botulinum , C. perfringens , and C. difficile , imply it could pose a risk to human health (2, 8, 12).…”

Section: Introductionmentioning

confidence: 99%

Clostridium perfringens spores in Polish honey samples

Grenda

Grabczak

Goldsztejn

et al. 2018

Journal of Veterinary Research

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IntroductionThe aim of this study was examination of honey samples collected from apiaries situated in all Polish provinces for occurrence of Clostridium spp., especially C. perfringens.Material and MethodsThe study was carried out on 240 honey samples (15 samples/province). Estimation of Clostridium titre, its cultures and C. perfringens isolate characterisation were performed according to the standard PN-R-64791:1994. A multiplex PCR method for detection of genes coding cpa (α toxin), cpb (β), cpb2 (β2), etx (ε), iap (ι), and cpe (enterotoxin) toxins was used.ResultsClostridium spp. was noticed in 56% (136/240) of samples, and its titres ranged between 0.1 g and 0.001 g. Clostridium perfringens occurrence was evidenced in 27.5% (66/240) of samples. All isolates were classified to toxinotype A.ConclusionsEvidence of a high number of positive samples with occurrence of Clostridium spp. indicates a potential risk to consumers’ health. The infective number of Clostridium spp. is unknown; however, the obtained results have shown that a risk assessment on the entire honey harvesting process should be made in order to ensure microbiological safety. Moreover, a detailed study should be undertaken on the antibiotic resistance of C. perfringens isolates from honey samples.

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Contamination of honey produced in the Republic of Kazakhstan with Clostridium botulinum

Cited by 10 publications

References 19 publications

Prevalence of C. botulinum and C. perfringens spores in food products available on Polish market

Prevalence of C. botulinum and C. perfringens spores in food products available on Polish market

Clostridium botulinumspores in Polish honey samples

Clostridium perfringens spores in Polish honey samples

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