Contamination assessment of a coastal lagoon (Ria de Aveiro, Portugal) using defence and damage biochemical indicators in gill of Liza aurata – An integrated biomarker approach

Oliveira, Miguel; Maria, V.L.; Ahmad, Iqbal; Serafim, A.; Bebianno, Maria João; Pacheco, Mário; Santos, M.A.

doi:10.1016/j.envpol.2008.10.019

Cited by 138 publications

(59 citation statements)

References 63 publications

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“…Therefore, it is important to know the response of this organ in fish exposed to environmental contaminants because its large surface area facilitates greater toxicant interaction and its detoxification system is not as robust as that of the liver [12]. In the present study, rainbow trout (Oncorhynchus mykiss), one of the widely used fish models in aquatic toxicology, was chosen as the test organism to determine the biochemical responses in fish gill after chronic exposure to propiconazole.…”

Section: -[ [2-(24-dichlorophenyl)-4-propyl-13-dioxolan-2-yl]mentioning

confidence: 99%

Biochemical responses in gills of rainbow trout exposed to propiconazole

Li¹,

Žlábek

Grabic

et al. 2010

Open Life Sciences

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The aim of this study is to investigate the toxic effect of PCZ, a triazole fungicide commonly present in surface and ground water, on the ROS defense system and Na+-K+-ATPase in gills of rainbow trout exposed to sublethal concentrations (0.2, 50 and 500 μg L−1) for 7, 20 and 30 days. After prolonged exposure of PCZ at higher test concentrations (50 and 500 μg L−1), oxidative stress was apparent as reflected by the significant higher ROS levels in fish gill, as well as the significant inhibition of SOD and CAT activities. In addition, Na+-K+-ATPase activities were significantly lower than those of the control with increasing PCZ concentration and prolonged exposure period. The results of this study indicate that chronic exposure to PCZ has altered multiple physiological indices in fish gill; however, before these parameters are used as unique biomarkers for monitoring residual pharmaceuticals in aquatic environments, more detailed laboratory experiments need to be performed.

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Section: -[ [2-(24-dichlorophenyl)-4-propyl-13-dioxolan-2-yl]mentioning

confidence: 99%

Biochemical responses in gills of rainbow trout exposed to propiconazole

Li¹,

Žlábek

Grabic

et al. 2010

Open Life Sciences

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“…Oxidative stress induces many types of adverse effects such as protein cleavage, loss of ions, membrane peroxidation, and DNA strand breakages. Oliveira et al (2009) showed that environmental pollutants may result in DNA damage directly by the action of parental compounds or their metabolites, or indirectly by the generated ROS. Our study demonstrated that nZnO resulted in DNA damage to larval zebrafish cells.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles

Boran

Ulutas²

2016

Dis. Aquat. Org.

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Engineered nanoparticles (NPs) can potentially generate adverse effects at the tissue, organ, cellular, subcellular, DNA, and protein levels due to their unique physico-chemical properties. Dissoluble NPs (e.g. nZnO) can be toxic in aquatic organisms. We compared effects of nZnO and corresponding concentrations of released Zn(II) by water-soluble ZnCl 2 on larval zebrafish Danio rerio (72 h post fertilization) by analyzing changes in expression levels of stressrelated genes ( p53, rad51, mt2) by qRT-PCR. Additionally, genotoxicity of nZnO and Zn(II) was assessed. The lethal concentrations for 50% mortality (LC 50 ) in larval zebrafish exposed for 96 h to 0 to 70 mg l −1 nZnO and Zn(II) were 21.37 ± 1.81 mg l −1 (95% CI) and 4.66 ± 0.11 mg l −1 , respectively. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed (96 h) to nZnO and Zn(II). DNA damage was higher in Zn(II)-than nZnO-exposed larvae. Induction of stress-related genes in larvae was complex and was not directly related to nZnO and Zn(II) concentrations, although there was significant induction in the mt2 gene of larvae exposed to Zn(II) and nZnO relative to controls. mt2 induction of 20.5 ± 1.9-fold and 2.5 ± 0.8-fold change (mean ± SEM) was observed in larvae at the highest Zn(II) and nZnO concentrations (3 and 6 mg l −1 ), respectively. The results suggest that toxicity associated with nZnO is primarily due to the release of Zn(II).KEY WORDS: Nanoscale ZnO · Zinc chloride · Genotoxicity · Stress-related genes · Danio rerio Resale or republication not permitted without written consent of the publisherDis Aquat Org 117: [205][206][207][208][209][210][211][212][213][214] 2016 for a number of enzymes. However, when present in excessive concentrations, this metal can cause adverse effects (Regoli & Principato 1995, Wilson et al. 2012. nZnO can partially but relatively rapidly dissolve in water, and the release of free Zn ions is the primary source of toxicity (Franklin et al. 2007, Blinova et al. 2010, Buerki-Thurnherr et al. 2013. Several studies have indicated that nZnO are toxic to fish: mortality, hatching inhibition, retarded growth, tissue injuries, and malformation have been reported in embryonic and larval zebrafish Danio rerio exposed to nZnO (Zhu et al. 2009, Bai et al. 2010, Zhao et al. 2013. Although some studies found that toxicity of nZnO could be mainly attributed to dissolved Zn ions (Franklin et al. 2007, Wong et al. 2010, toxicity of nZnO cannot always be explained by the dissolved Zn ions (Bai et al. 2010.Particle-induced generation of reactive oxygen species (ROS) and dissolution to ionic Zn represent the primary modes of action for nZnO toxicity across all species tested (Ma et al. 2013). Indeed, ROS induction triggering an oxidative stress response has become a widely accepted paradigm for cellular effects of NPs (Nel et al. 2006, Zhu et al. 2009, Song et al. 2010, Sharma et al. 2012. Excessive production of ROS can induce pro-inflammatory and cytotoxic effects (Nel et al. 200...

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“…Over decades, this lagoon has been the main receptor of anthropogenic discharges resulting mainly from chlor-alkali (Pereira et al 1997) and pulp/paper plants , harbour and dry-dock activities (Barroso et al 2000;Pacheco et al 2005), municipal and domestic effluents and chemicals used in agriculture (Monteiro et al 2007). Though in the year 2000 point source discharges were diverted through a submarine outlet 2.5 km far from the sea coast, recent studies have demonstrated the continuation of critical areas (Santos et al 2004Ahmad et al 2008;Oliveira et al 2009), revealing the imperative need of an effective biomonitoring programme. Thus, the goal of the present study was the assessment of Ria de Aveiro environmental status, as a coastal lagoon prototype, focusing simultaneously on kidney antioxidant defences (non-protein thiols-NPT, total glutathione-GSHt, catalase-CAT, glutathione peroxidase-GPx, glutathione S-transferase-GST, glutathione reductase-GR) and damage (LPO and DNA integrity loss) responses.…”

Section: Introductionmentioning

confidence: 97%

Monitoring pollution of coastal lagoon using Liza aurata kidney oxidative stress and genetic endpoints: an integrated biomarker approach

et al. 2009

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Despite the importance of fish kidney in several functions (immune, metabolism and excretion of xenobiotics) its use in coastal water biomonitoring focusing on protection and damage is scarce. Five critical sites in Ria de Aveiro (Portugal; Barra--BAR, Gafanha--GAF, Rio Novo do Principe--RIO; Laranjo--LAR and Vagos--VAG) were assessed in comparison to a reference site (Torreira--TOR), focusing on Liza aurata kidney antioxidant defences versus damage responses. Non protein thiols were higher at RIO (near a former bleached kraft pulp mill effluent) and total glutathione at RIO, LAR (mercury contaminated) and VAG (polycyclic aromatic hydrocarbons contaminated). Catalase and glutathione S-transferase activities were higher at RIO and LAR whereas no differences were found in glutathione peroxidase activity. However, glutathione reductase was higher at BAR (subject to naval traffic), GAF (harbour water area), RIO and LAR. No peroxidative damage was observed despite the decreased DNA integrity at RIO and VAG. The integrated biomarker response index ranked impacted sites as: LAR>RIO>BAR>GAF>VAG>TOR.

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Contamination assessment of a coastal lagoon (Ria de Aveiro, Portugal) using defence and damage biochemical indicators in gill of Liza aurata – An integrated biomarker approach

Cited by 138 publications

References 63 publications

Biochemical responses in gills of rainbow trout exposed to propiconazole

Biochemical responses in gills of rainbow trout exposed to propiconazole

Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles

Monitoring pollution of coastal lagoon using Liza aurata kidney oxidative stress and genetic endpoints: an integrated biomarker approach

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