There are three kinds of loading conditions applied to the O-ring, The first loading condition is the case in which uniform squeeze rates are applied to the upper side and the lower side of the O-ring (the strain condition). The second loading condition is the case in which uniform squeeze rates are applied to the upper side and the lower side of the Oring and other squeeze rates are applied to the front side of the O-ring. The third loading condition is the case in which uniform squeeze rates are applied to the upper side and the lower side of the O-ring, other squeeze rates are applied to the front side of the O-ring, and internal pressures are applied to another front side of the O-ring(loading condition is the combination of stress condition and the strain condition). In this research, a new photoelastic experimental hybrid method under the third loading condition was developed and it was verified. The stresses of the O-ring under the third loading condition were analyzed by the new photoelastic experimental hybrid method developed in this research. The internal pressures applied to the O-ring were 0.98 MPa, 1.96 MPa, 2.94 MPa and 3.92 MPa.