Anthocyanins are bioactive compounds naturally found
in a variety of leaves, fruits, and vegetables. Anthocyanin pigments
undergo a modification in their chemical structure when exposed to
different concentrations of hydrogen ions, and they were extensively
studied to be used as active elements in biocompatible pH sensors.
The ocular pH is a significant parameter to assess the ocular physiology
in cases of postocular surgery, keratoconjunctivitis, and ocular rosacea.
Contact lenses have the potential to be used as medical diagnostic
devices for in situ continuous monitoring of the ocular physiology.
Here, anthocyanin-functionalized contact lenses were developed as
wearable sensors to monitor the ocular pH. Anthocyanin pigments were
extracted from Brassica oleracea and
used to functionalize the polymeric matrices of commercial soft contact
lenses by soaking and drop-casting processes. Contact lenses responded
to the physiological ocular pH of 6.5, 7.0, and 7.5, exhibiting a
systematic color shift from pink (pH 6.5) to purple (pH 7.0) and blue
(pH 7.5). The functionalization of contact lens sensors was evaluated
as a function of the dye concentration. Quantitative values were obtained
by comparing the RGB triplets of the colors obtained with the naturally
extracted dye and with delphinidin chloride dye in 0.0 to 1.5 mmol
Lâ1 aqueous solution. The functionalization of contact
lenses was studied as a function of the soaking time, resulting in
best results when soaking for 24 h. The dye leakage from the contact
lenses in deionized water was evaluated, and a negligible leakage
after 18 h was observed. Poly-2-hydroxy ethylmethacrylate contact
lenses were fabricated and cross-linked with anthocyanin dye, resulting
in a slight color shift upon pH changes from 6.5 to 7.4. Contact lens
pH sensors may be used to continuously monitor the ocular pH at point-of-care
settings.