Contact chemostimuli in the mating behaviour of the cattle tick,Boophilus microplus

Bruyne, Marien de; Guérin, Patrick M.

doi:10.1002/(sici)1520-6327(1998)39:2<65::aid-arch3>3.0.co;2-w

Cited by 24 publications

(9 citation statements)

References 18 publications

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“…Compared to insects, chemical communication in other arthropods, particularly Chelicerata, is poorly understood. Most of the studies are focused on morphology 11 and electrophysiology [12][13][14] while several papers report on the identification of putative semiochemicals 8,9,15-18 . Gustation and olfaction take place in sensilla, which are located on mouthparts and forelegs in ticks and mites. In Varroa the main olfactory organ, referred to as pit organ, is located on forelegs and presents nine olfactory hairs, which are morphologically similar to insect sensilla basiconica 19,20 .…”

Section: Communication Betweenmentioning

confidence: 99%

Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: A comprehensive examination of the potential carriers for semiochemicals

Iovinella

McAfee

Mastrobuoni

et al. 2018

Journal of Proteomics

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We have performed a proteomic analysis on chemosensory organs of Varroa destructor, the honey bee mite, in order to identify putative soluble carriers for pheromones and other olfactory cues emitted by the host. In particular, we have analysed forelegs, mouthparts (palps, chelicera and hypostome) and the second pair of legs (as control tissue) in reproductive and phoretic stages of the Varroa life cycle. We identified 958 Varroa proteins, most of them common to the different organs and stages. Sequence analysis shows that four proteins can be assigned to the odorant-binding protein (OBP)-like class, which bear some similarity to insect OBPs, but so far have only been reported in some Chelicerata. In addition, we have detected the presence of two proteins belonging to the Niemann-Pick family, type C2 (NPC2), which have also been suggested as semiochemical carriers. Biological significance: The mite Varroa destructor is the major parasite of the honey bee and is responsible for great economical losses. The biochemical tools used by Varroa to detect semiochemicals produced by the host are still largely unknown. This work contributes to understand the molecular basis of olfaction in Varroa and, more generally, how detection of semiochemicals has evolved in terrestrial non-hexapod Arthropoda. Moreover, the identification of molecular carriers involved in olfaction can contribute to the development of control strategies for this important parasite.

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Section: Communication Betweenmentioning

confidence: 99%

Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: A comprehensive examination of the potential carriers for semiochemicals

Iovinella

McAfee

Mastrobuoni

et al. 2018

Journal of Proteomics

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“…Neuronal electrical signals have been detected when these sensilla are exposed to specific chemicals. Host odors and mating pheromones are detected by sensilla on the Haller's organ (Waladde, ; Sonenshine et al ., ; Steullet & Guerin, ,b; Soares & Borges, ); mounting pheromones are detected by sensilla on the palps (De Bruyne & Guerin, ); and phagostimulants are detected by sensilla on the chelicerae (Sonenshine et al ., ; Soares et al ., ). Thus, the fore tarsi, palps, and chelicerae are considered to be the main tick chemosensory organs.…”

Section: Introductionmentioning

confidence: 99%

The chemosensory appendage proteome of Amblyomma americanum (Acari: Ixodidae) reveals putative odorant‐binding and other chemoreception‐related proteins

et al. 2016

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Proteomic analyses were done on 2 chemosensory appendages of the lone star tick, Amblyomma americanum. Proteins in the fore tarsi, which contain the olfactory Haller's organ, and in the palps, that include gustatory sensilla, were compared with proteins in the third tarsi. Also, male and female ticks were compared. Proteins were identified by sequence similarity to known proteins, and by 3-dimensional homology modeling. Proteomic data were also compared with organ-specific transcriptomes from the tick Rhipicephalus microplus. The fore tarsi express a lipocalin not found in the third tarsi or palps. The fore tarsi and palps abundantly express 2 proteins, which are similar to insect odorant-binding proteins (OBPs). Compared with insect OBPs, the tick OBP-like sequences lacked the cysteine absent in C-minus OBPs, and 1 tick OBP-like sequence had additional cysteines that were similar to C-plus OBPs. Four proteins similar to the antibiotic protein microplusin were found: 2 exclusively expressed in the fore tarsi and 1 exclusively expressed in the palps. These proteins lack the microplusin copper-binding site, but they are modeled to have a significant internal cavity, potentially a ligand-binding site. Proteins similar to the dust mite allergens Der p7 and Der f 7 were found differentially expressed in female fore tarsi. A protein exclusively expressed in the fore tarsi has similarities to Neto, which is known to be involved in clustering of ionotropic glutamate receptors. These results constitute the first report of OBP-like protein sequences in ticks and point to several research avenues on tick chemosensory reception.

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“…Vapours of these compounds presented in an air stream attract adults of this species, both individually and in binary blends (McMahon & Guerin, 2000a). Gustation is also known to mediate several aspects of tick biology, such as mating (de Bruyne & Guerin, 1998), feeding (Waladde et al, 1979) and aggregation (Leahy et al, 1973;Dusba´bek et al, 1991). Recently, Grenacher et al (2001) demonstrated that the arrestment behaviour in the sheep tick, Ixodes ricinus (Linnaeus), on its own faeces is mediated by gustatory receptor cells housed in terminal wall-pore sensilla on the first leg tarsus.…”

Section: Introductionmentioning

confidence: 99%

In vitro assays for repellents and deterrents for ticks: differing effects of products when tested with attractant or arrestment stimuli

McMahon

Kröber

Guérin

2003

Medical Vet Entomology

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Abstract. Most in vivo and in vitro tests with repellents or deterrents against ticks have not considered which sensory channel is being targeted. We have recorded the responses of two hard tick species (Acari: Ixodidae) in vitro to determine if such products can disrupt the perception of an attractant in a repellent assay or the perception of an arrestment stimulus in a deterrent assay. Ethyl butylacetylaminopropionate (EBAAP), N,N-diethyl-methyl-benzamide (deet), permethrin and indalone were chosen to test their capacity to inhibit the attraction of Amblyomma variegatum Fabricius to its aggregation-attachment pheromone. Vapours of each test product plus those from a synthetic blend of the pheromone were delivered to the walking tick in an air stream on a locomotion compensator. Neither EBAAP, deet, permethrin nor indalone could inhibit attraction of A. variegatum even when each of the test products was delivered at 10 6 times the pheromone. Indalone did decrease the attraction of A. variegatum to the pheromone and induced repulsion of A. variegatum when presented on its own in the air stream. The effect of permethrin, a sodium channel blocker, was also tested in a deterrent assay measuring the arrestment of Ixodes ricinus (L.) adults on its own faeces and faecal constituents. Permethrin deterred arrestment at doses of 670 fg/cm 2 to 67 ng/cm 2 , i.e. at levels five times lower than the dose of chemostimuli present in the arrestment stimulus. This sensitivity to permethrin suggests that it acts via the contact chemoreception channel.

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Contact chemostimuli in the mating behaviour of the cattle tick,Boophilus microplus

Cited by 24 publications

References 18 publications

Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: A comprehensive examination of the potential carriers for semiochemicals

Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: A comprehensive examination of the potential carriers for semiochemicals

The chemosensory appendage proteome of Amblyomma americanum (Acari: Ixodidae) reveals putative odorant‐binding and other chemoreception‐related proteins

In vitro assays for repellents and deterrents for ticks: differing effects of products when tested with attractant or arrestment stimuli

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