Consumption of yogurt containing probiotic Bifidobacterium lactis reduces Streptococcus mutans in orthodontic patients

Abstract

Background: Probiotic bacteria is commonly used as a food supplement intended to benefit the host by improving intestinal bacterial balance. Probiotics have also been investigated from the perspective of oral health. Objectives: The purpose of this study was to investigate the effect of daily intake of yogurt containing probiotic Bifidobacterium animalis subsp. lactis BB-12 (B. lactis) on salivary Streptococcus mutans (S. mutans) counts in patients undergoing fixed orthodontic treatment. Methods: Saliva samples were collected from each subject (n = 7; mean age, 21 years) using spitting method in centrifuge tubes at baseline and two weeks after daily probiotic yogurt consumption. B. lactis BB-12 and S. mutans ATCC 25175 were cultured in BHI-broth (37ºC, anaerobic conditions). After 48 hours incubation, the number of colonies on each dilution plate was used to extrapolate a standard curve. The total number of target DNA molecules were identified using Real-Time PCR followed by SYBR Green reagents and 16S rRNA gene specific primers S. mutans and B. lactis BB-12. Data were analyzed statistically using paired-sample t-tests. Results: Statistical evaluation indicated that there was a significant reduction in the presence of S. mutans before probiotic yogurt consumption, (4.73 ± 1.43) log10 CFU/mL and after two weeks of daily consumption of probiotic yogurt, (4.03 ± 0.77) log10 CFU/mL, p = 0.001. Moreover, no B. lactis was found in the saliva of any of the subjects before probiotic consumption, but after two weeks of consumption, B. lactis was found in the saliva of four subjects. Conclusions: Consuming probiotic yogurt containing B. lactis reduced the quantity of S. mutans in the saliva of subjects during fixed orthodontic treatment. Thus, the probiotic bacteria could be beneficial in improving oral health.

The anti-inflammatory effects of glycerol-supplemented probiotic lactobacillus reuteri on infected epithelial cells In vitro

“…A recent study showed that probiotic yogurt containing Bifidobacterium lactis BB-12 consumption can reduce the levels of S. mutans as pathogenic bacteria in the saliva. [ 8 ] Among these bacteria, probiotics are widely used. [ 1 ] Lactobacillus reuteri , as a probiotic bacterium, also has been proven to enhance oral health.…”

Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on S. mutans Growth, Biofilm Formation, and L. rhamnosus Growth

“…Probiotic supplements and a prebiotic diet are encouraged for children who are deprived of natural breastfeeding to restore their oral microbiome and ensure good oral and dental health [ 50 , 51 ]. To reverse the undesired changes in the microbiota’s composition and function brought on by antibiotic treatments or caesarean delivery, a probiotic mixture in addition to at least partial breastfeeding is highly recommended [ 52 ].…”

Persistent Endodontics Pathogens Biofilm Inhibited by Lactobacillus reuteri Indonesian Strain Lactobacillus reuteri Indonesian

“…This yogurt consumption could be used to reduce the prevalence of enamel demineralization among patients with fixed orthodontic appliances. 33,34 Probiotics can also enhance oral health through immune system modulation. L. reuteri significantly reduced the production of the pro-inflammatory cytokine, interleukin (IL)-8, and induced the production of human beta defensin-2 in epithelial cells induced by infection with S. mutans and P. gingivalis.…”