Consumption of field-realistic doses of a widely used mito-toxic fungicide reduces thorax mass but does not negatively impact flight capacities of the honey bee (Apis mellifera)

Glass, Jordan R.; Fisher, Adrian; Fewell, Jennifer H.; DeGrandi-Hoffman, Gloria; Öztürk, Cahit; Harrison, Jon F.

doi:10.1016/j.envpol.2021.116533

Cited by 15 publications

(12 citation statements)

References 67 publications

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“…However, supporting evidence for this hypothesis is mixed, as some studies have reported no effects of Pristine on carbon dioxide production and thorax temperatures during flight [54]. Glass et al [58] reported a negative effect of Pristine on maximal flight performance, but only when fed at 230 ppm, which is 10 times higher than the concentration used in our experiments.…”

Section: Discussioncontrasting

confidence: 43%

Synergistic negative effects between a fungicide and high temperatures on homing behaviours in honeybees

DesJardins,

Chester,

Ozturk

et al. 2024

Proc. R. Soc. B.

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Interactions between environmental stressors may contribute to ongoing pollinator declines, but have not been extensively studied. Here, we examined the interaction between the agricultural fungicide Pristine (active ingredients: 25.2% boscalid, 12.8% pyraclostrobin) and high temperatures on critical honeybee behaviours. We have previously shown that consumption of field-realistic levels of this fungicide shortens worker lifespan in the field and impairs associative learning performance in a laboratory-based assay. We hypothesized that Pristine would also impair homing and foraging behaviours in the field, and that an interaction with hot weather would exacerbate this effect. Both field-relevant Pristine exposure and higher air temperatures reduced the probability of successful return on their own. Together, the two factors synergistically reduced the probability of return and increased the time required for bees to return to the hive. Pristine did not affect the masses of pollen or volumes of nectar or water brought back to the hive by foragers, and it did not affect the ratio of forager types in a colony. However, Pristine-fed bees brought more concentrated nectar back to the hive. As both agrochemical usage and heat waves increase, additive and synergistic negative effects may pose major threats to pollinators and sustainable agriculture.

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Section: Discussioncontrasting

confidence: 43%

Synergistic negative effects between a fungicide and high temperatures on homing behaviours in honeybees

DesJardins,

Chester,

Ozturk

et al. 2024

Proc. R. Soc. B.

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“…The neonicotinoid pesticide imidacloprid reduced range and period of bumble bee flights (Kenna et al, 2019). High, but not field-realistic concentrations of a mito-toxic fungicide did impair honey bee flight capacity in a flight chamber (Glass et al, 2021). These authors by the way also reported a reduced thorax mass of bees reared under field-realistic fungicide concentrations.…”

Section: Figurementioning

confidence: 90%

Flight performance of pollen starved honey bees and incomplete compensation through ingestion after early life pollen deprivation

Brodschneider

Omar

2022

Front. Physiol.

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We investigated the effect of adult honey bee pollen nutrition on the flight performance of honey bees. Therefore, caged bees were allowed to perform 30 min of defecation/training flights every second day before flight performance of pollen-fed bees and pollen-deprived bees older than 16 days were compared in a flight mill. We first fed 10 µL of 1 M glucose solution to bees, and after they metabolized this during flight, they were fed 10 µL of 2 M glucose solution for a second flight test. Pollen-deprived bees flew longer and further than pollen-fed bees in both flights. Pollen-fed bees flew faster in the early period at the beginning of flights, whereas pollen-deprived bees were faster in the final phases. Pollen-fed bees were able to raise their maximum flight speed in 2 M glucose solution flights, whereas pollen-constraint bees were not. The two groups did not differ in abdomen fresh weight, but the fresh weight of the head and thorax and dry weight of the head, thorax and abdomen were higher in pollen-fed bees. In a second experiment, we constrained pollen consumption of caged bees during the first 7 days and compared daily consumption of bees from day 8–16 to consumption of bees unrestricted in pollen. We found that pollen-deprived bees perceive the pollen shortage and try to compensate for their needs by consuming significantly more pollen at the later phase of their life than pollen-fed bees of the same age. Still, bees constrained from pollen in the first 7 days did only reach 51.1% of the lifetime consumption of unconstrained bees. This shows that bees can sense the need for essential nutrients from pollen, but their physiological apparatus does not allow them to fully compensate for their early life constraint. Pollen deprivation only in the first 7 days of worker life likewise significantly reduced fresh and dry weights of the body sections (head, thorax, and abdomen) and survival. This underlines the importance of protein consumption in a short critical period early in adult bees’ lives for their development and their performance later in life.

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“…Flight behaviour was categorized based on ability, duration and control (i.e. quality; [25]). Flight was categorized and ranked as: 1, no flight; 2, flapping wings with brief periods of flight (less than 3 s); 3, intermittent flight characterized by frequent crashing (i.e.…”

Section: Methodsmentioning

confidence: 99%

The thermal performance curve for aerobic metabolism of a flying endotherm

Glass

Harrison

2022

Proc. R. Soc. B.

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Performance benefits of stable, warm muscles are believed to be important for the evolution of endothermy in mammals, birds and flying insects. However, thermal performance curves have never been measured for a free-flying endotherm, as it is challenging to vary body temperatures of these animals, and maximal flight performance is difficult to elicit. We varied air temperatures and gas densities to manipulate thoracic temperatures of flying honeybees from 29°C to 44°C, with low air densities used to increase flight metabolic rates to maximal values. Honeybees showed a clear thermal performance curve with an optimal temperature of 39°C. Maximal flight metabolic rates increased by approximately 2% per 1°C increase in thoracic temperature at suboptimal thoracic temperatures, but decreased approximately 5% per 1°C increase as the bees continued to heat up. This study provides the first quantification of the maximal metabolic performance benefit of thermoregulation in an endotherm. These data directly support aerobic capacity models for benefits of thermoregulation in honeybees, and suggest that improved aerobic capacity probably contributes to the multiple origins of endothermic heterothermy in bees and other insects.

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Consumption of field-realistic doses of a widely used mito-toxic fungicide reduces thorax mass but does not negatively impact flight capacities of the honey bee (Apis mellifera)

Cited by 15 publications

References 67 publications

Synergistic negative effects between a fungicide and high temperatures on homing behaviours in honeybees

Synergistic negative effects between a fungicide and high temperatures on homing behaviours in honeybees

Flight performance of pollen starved honey bees and incomplete compensation through ingestion after early life pollen deprivation

The thermal performance curve for aerobic metabolism of a flying endotherm

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