Consumption of cow milk and egg by lactating women and the presence of beta-lactoglobulin and ovalbumin in breast milk

Fukushima, Yoichi; Kawata, Yoichi; Onda, Takekazu; Kitagawa, Michihiro

doi:10.1093/ajcn/65.1.30

Cited by 88 publications

(72 citation statements)

References 24 publications

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“…However, bLG has been detected in considerable amounts in the majority of lactating mothers consuming milk products [17]. In contrast, it is not detectable in extensively hydrolyzed formulae, while residual bLG was found in some, but not all partially hydrolyzed products [18]. Immunophenotype markers of bLG–primed PBMC showed no significant differences, with the exception of CD23 that was increased in breast–fed infants.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Lymphocyte Proliferating Cell Nuclear Antigen Expression with Dietary Cow’s Milk Antigen Load in Infants with Allergy to Cow’s Milk

Papadopoulos

Syrigou

Bossios

et al. 1999

Int Arch Allergy Immunol

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Background: Controversial results have been reported on the participation and diagnostic value of lymphocyte reactivity in cow’s milk (CM) allergy. In this study, we used a specific nuclear marker to evaluate lymphocyte proliferation in IgE–mediated CM allergy in infants, and examine its relation with diets containing different CM antigen loads. Methods: Infants with IgE–mediated CM allergy, as assessed by open provocation and RAST, were grouped according to their exclusive diet, either CM formulae, breast feeding, or hydrolysed whey formulae. A group of non–atopic infants receiving CM was also examined. Lymphocyte proliferation to β–lactoglobulin was evaluated by quantitation of the proliferating cell nuclear antigen (PCNA) expression in peripheral blood mononuclear cells, by flow cytometry. Immunophenotypic surface markers were also examined. Results: A marked difference of PCNA expression between CM–fed allergic infants and healthy controls was observed (p<0.001). In this setting, PCNA expression ≥10% was highly specific and sensitive as a marker of CM allergy in CM–fed infants. Moreover, a significant correlation (p<0.001) between antigen load and PCNA was established in CM–allergic infants under different diets, higher values obtained with increasing antigen loads. In addition, within the group fed hydrolyzed formulae, low–molecular–weight products resulted in marginally lower PCNA expression than higher–molecular–weight formulae. No differences in immunophenotype were found, with the exception of a higher CD23 expression in the breast–fed group. Conclusions: PCNA could be a useful marker in the assessment of lymphocyte proliferation to CM antigens. Low CM antigen diets are related with reduced lymphocyte reactivity, which may partly explain the clinical benefit observed with such diets.

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Section: Discussionmentioning

confidence: 99%

Correlation of Lymphocyte Proliferating Cell Nuclear Antigen Expression with Dietary Cow’s Milk Antigen Load in Infants with Allergy to Cow’s Milk

Papadopoulos

Syrigou

Bossios

et al. 1999

Int Arch Allergy Immunol

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“…In human breast milk the presence of β-lactoglobulin from cow's milk, ovalbumin and ovomucoid from eggs, and gliadin from wheat has been demonstrated in previous studies [1,2,3,4,5]. In terms of peanut allergy, recent data from the German Registry of Anaphylaxis revealed that peanut allergy is no longer a life-threatening problem of the USA only, but is of growing significance in German-speaking countries as well [6].…”

Section: Introductionmentioning

confidence: 87%

Detection of the Peanut Allergens Ara h 2 and Ara h 6 in Human Breast Milk: Development of 2 Sensitive and Specific Sandwich ELISA Assays

Schocker

Scharf

Kull

et al. 2017

Int Arch Allergy Immunol

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Background: Little is known about breast milk as a vehicle for tolerance development or sensitization to peanuts very early in life. Thus, well-characterized and highly sensitive detection systems for the reliable determination of peanut allergens in breast milk are mandatory. Methods: For the quantification of the marker allergens Ara h 2 and Ara h 6 in the low nanogram per milliliter range in breast milk samples of a German cohort, sensitive and highly specific sandwich ELISAs were optimized and validated. Results: The Ara h 2 ELISA revealed a limit of detection (LOD) of 1.3 ng Ara h 2/mL and a quantification range of 2.3-250 ng/mL, the Ara h 6 ELISA showed an LOD of 0.7 ng/mL and a working range of 1.1-14.4 ng/mL. The assays showed no relevant cross-reactivity against other potentially cross-reactive legume, seed, and tree nut extracts (<0.01%, except for Ara h 1 in the Ara h 2 ELISA <0.1%). Ara h 2 was detectable in breast milk samples from 14/40 (35%) of the participants in concentrations from 2.3 to 184 ng/mL, Ara h 6 appeared in 9/40 (22.5%) of the lactating mothers between 1.1 and 9.7 ng/mL, and 1 highly positive sample with 79 ng/mL. Both allergens appeared at the same time points, but Ara h 6 in lower concentrations than Ara h 2. Conclusions: Sensitive and specific diagnostic tools for the determination of Ara h 2 and Ara h 6 in human breast milk were established. The kinetics of secreted Ara h 2 and Ara h 6 seem to be similar but with a difference in concentration. Follow-up investigations on their tolerogenic or sensitizing properties in breast milk become now accessible.

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“…[16][17][18][19] The time from ingestion to peak concentration of ␤-lactoglobulin in breast milk was 1 to 6 hours. Two major egg allergens, ovalbumin and ovomucoid, are readily transferred to breast milk, 16,20,21 with peak concentrations attained 2 to 6 hours after ingestion. Similarly, gliadin from dietary wheat is detectable within 2 to 4 hours of ingestion.…”

Section: Commentmentioning

confidence: 99%

“…22,23 Concentrations of bovine ␤-lactoglobulin (5-800 ng/mL), egg ovalbumin (200 pg/mL-6 ng/mL) and ovomucoid (0-2.88 ng/mL), and wheat gliadin (5-95 ng/mL) are dependent on the amount ingested, and peak concentrations in breast milk are generally in the nanogram per milliliter range. 16,[20][21][22][23] Peanut proteins have long been suspected to be secreted but have never been identified in breast milk. We now provide definitive evidence for secretion of peanut protein into breast milk of lactating women.…”

Section: Commentmentioning

confidence: 99%

Detection of Peanut Allergens in Breast Milk of Lactating Women

Vadas

Wai

Burks³

et al. 2001

JAMA

186

129

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Consumption of cow milk and egg by lactating women and the presence of beta-lactoglobulin and ovalbumin in breast milk

Cited by 88 publications

References 24 publications

Correlation of Lymphocyte Proliferating Cell Nuclear Antigen Expression with Dietary Cow’s Milk Antigen Load in Infants with Allergy to Cow’s Milk

Correlation of Lymphocyte Proliferating Cell Nuclear Antigen Expression with Dietary Cow’s Milk Antigen Load in Infants with Allergy to Cow’s Milk

Detection of the Peanut Allergens Ara h 2 and Ara h 6 in Human Breast Milk: Development of 2 Sensitive and Specific Sandwich ELISA Assays

Detection of Peanut Allergens in Breast Milk of Lactating Women

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