Construction, purification, and characterization of a chimeric TH1 antagonist

The presence of epidural IFNgamma-immunoreactivity corresponding to >10 pg/mL predicted significant pain relief after epidural steroid injection with >95% accuracy. These results suggest that IFNgamma may be part of a biochemical cascade triggering pain in sciatica; IFNgamma-immunoreactivity may aid as a biomarker for predicting the response to steroid therapy and/or surgical intervention, and may serve as a future therapeutic target.

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Analysis of the relationship between end-to-end distance and activity of single-chain antibody against colorectal carcinoma

Zhang

Liu

Shang

et al. 2012

Theor Biol Med Model

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We investigated the relationship of End-to-end distance between VH and VL with different peptide linkers and the activity of single-chain antibodies by computer-aided simulation. First, we developed (G4S)n (where n = 1-9) as the linker to connect VH and VL, and estimated the 3D structure of single-chain Fv antibody (scFv) by homologous modeling. After molecular models were evaluated and optimized, the coordinate system of every protein was built and unified into one coordinate system, and End-to-end distances calculated using 3D space coordinates. After expression and purification of scFv-n with (G4S)n as n = 1, 3, 5, 7 or 9, the immunoreactivity of purified ND-1 scFv-n was determined by ELISA. A multi-factorial relationship model was employed to analyze the structural factors affecting scFv: rn=[]AB()n−boldABO2+[]CD()n−CDO2+[]BC()n−BCst2. The relationship between immunoreactivity and r-values revealed that fusion protein structure approached the desired state when the r-value = 3. The immunoreactivity declined as the r-value increased, but when the r-value exceeded a certain threshold, it stabilized. We used a linear relationship to analyze structural factors affecting scFv immunoreactivity.

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Construction, purification, and characterization of a chimeric TH1 antagonist

Abstract: Background: TH1 immune response antagonism is a desirable approach to mitigate some autoimmune and inflammatory reactions during the course of several diseases where IL-2 and IFN-γ are two central players. Therefore, the neutralization of both cytokines could provide beneficial

Cited by 3 publications

References 45 publications

Design and optimization of a linker for fusion protein construction

Design and optimization of a linker for fusion protein construction

Epidural Interferon Gamma-Immunoreactivity

Analysis of the relationship between end-to-end distance and activity of single-chain antibody against colorectal carcinoma

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