Construction of tissue arrays without prefabricated recipient paraffin block experience of a novel technique in resource poor settings

Pathak, Gayatri S; Deshmukh, Sanjay D; Ashturkar, Amrut V

doi:10.4103/0377-4929.85139

Cited by 11 publications

(5 citation statements)

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“…Manual construction of TMAs without using recipient blocks has also been described, in which tissue cores are attached to the bottom of the block mold using double-sided adhesive tape on a thin layer of soft wax 5,8,11,12. In this method, all the tissue cores from the donor blocks are vertically attached to the bottom of the block mold before melted paraffin is poured in to fill the residual spaces.…”

Section: Discussionmentioning

confidence: 99%

Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks

et al. 2012

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BackgroundAdvances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost.MethodsOrdinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks.ResultsBy using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes.ConclusionsConstruction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices.

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Section: Discussionmentioning

confidence: 99%

Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks

et al. 2012

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“…Hypodermic needle with lateral opening, attached to hand-press grommet insert machine 0,6 mm, 325 (25x13) 7,5 hr <1 100 USD Pathak et al [16] Boring holes in bare paraffin block using mini hand drill and microcompound [17] Vogel [18] Boring holes guided by dot-grid paper directly attached to the bare paraffin block …”

Section: Resultsmentioning

confidence: 99%

“…(Table 1). [12][13][14][15][16][17][18][19] However, in most of cases, the development of these tecnhniques is related to personal laboratory technicians' skillfulness or require of specialized instruments, making the production of TMAs more laborious. Author's objective was to overcome this problem by offering both an inexpensive and flexible method that could be modified according to the requirements of the lab, with respect to the number of cores in one block.…”

Section: Discussionmentioning

confidence: 99%

Fast and inexpensive self-made tissue microarray for immunohistochemical and in situ hybridization studies: examples with bladder cancer

Komina

Petruševska²

2017

APALM

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Background:The tissue microarray (TMA), first described by Battifora and first implemented in 1998 by Kononen et al, is a powerful research tool, allowing simultaneous analysis of specimens from a large number of cases on one slide. Unfortunately, commercial aray instruments are very expensive and thus not suitable for laboratories with limited funds. We describe simple and cost-effective method for constructing of manual TMA that can be performed by any anatomic pathology laboratory, requiring minimum skill and time.Methods: Skin punch biopsy needle of 2-mm diameter was used for extracting cores from 15 positive control breast cancer cases, 15 normal bladder tissue and 30 muscle-invasive bladder cancer and for injecting into recipient block. Result:We constructed TMA block using skin punch biopsy needle of 2-mm, and succesfulfully performed HER2 immunodetection and Chromogenic In Situ Hybridization study, without substantial tissue loss. 10% of our tumor cases exhibited Her-2 neu overexpression. An open source software programme "ТМА-Ј" was applied to facilitate management, viewing, analysis of tissue microarray images and associated clinico-pathology data. Conclusion:This method could be done by any pathology laboratory and represents good and reliable alternative for commercially available, expensive devices and software solutions.

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“…However, as a result of not using a prefabricated recipient block, core tilt and flotation were encountered during the embedding process, easily tackled by dispensing the molten paraffin in a controlled manner. Alternatively, to avoid these difficulties, different methods can be used during the embedding process, such as doublesided adhesive tape, encasement in a drinking straw, and paper molds (23,24,(25)(26)(27). Such additional steps demand specific experience and technical skills that are time-consuming.…”

Section: Discussionmentioning

confidence: 99%

A Simplified Method of Manually Constructing Small Format Tissue Microarray for Use in Resource-Constrained Settings

Palo

2023

Iran J Pathol

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Background & Objective: Tissue microarray (TMA) is a method of harvesting small tissue cores from a number of donor paraffin tissue blocks and arraying them in a recipient paraffin block. It has numerous advantages and applications but is expensive. This study aimed to develop a simple yet efficient method of manual, small-format TMA block construction. Methods: Disposable skin punch biopsy needles were used to manually core out 4-mm cylinders from the archival donor blocks comprising tissue from 60 thyroidectomy specimens. These cores were oriented in the embedding cassette in accordance with the grid design. The molten wax was slowly dispensed and allowed to be set. Sectioning, mounting, and hematoxylin and eosin (H&E) staining were performed by a conventional method. Immunohistochemical studies, using HBME-1, CK19, and S100 antibodies, were also performed on these tissue array sections. Results: There was no core loss during processing. Technical issues like core tilt and floatation were easily tackled. Morphological identification, histological typing, and immunohistochemical analysis could be satisfactorily performed in these TMA sections. Donor blocks did not break after punching. Conclusion: This TMA construction method is simple, feasible, easily reproducible, and time-saving. It can serve as an excellent cost-effective alternative for resource-poor laboratories for carrying out immunohistochemical studies.

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Construction of tissue arrays without prefabricated recipient paraffin block experience of a novel technique in resource poor settings

Cited by 11 publications

References 0 publications

Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks

Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks

Fast and inexpensive self-made tissue microarray for immunohistochemical and in situ hybridization studies: examples with bladder cancer

A Simplified Method of Manually Constructing Small Format Tissue Microarray for Use in Resource-Constrained Settings

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