Construction of SSR-based chromosome map in bunching onion (Allium fistulosum)

Tsukazaki, Hikaru; Yamashita, Kazuo; Yaguchi, Shigenori; Masuzaki, Shin-ichi; Fukuoka, Hiroyuki; Yonemaru, Jun‐ichi; Kanamori, Hajime; Kono, Izumi; Hang, Tran Thi Minh; Shigyo, Masayoshi; Kojima, Akihiko; Wako, Tadayuki

doi:10.1007/s00122-008-0849-5

Cited by 39 publications

(41 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the degree of genetic trait expression depends on the interaction of genotype with environment and farming practices. With this respect, Tsukazaki et al (2008) constructed a new linkage map on Allium fistulosum, the first one mainly based on SSR markers in the genus Allium, which will be useful for genetic studies such as QTL analysis of agronomic traits.…”

Section: Genotype and Environmentmentioning

confidence: 99%

Interactions among genotype, environment and agronomic practices on production and quality of storage onion (Allium cepa L.) - A review

Sękara¹,

Pokluda²,

Vacchio³

et al. 2017

Hortic. Sci.

View full text Add to dashboard Cite

Sekara A., Pokluda R., Del Vacchio L., Somma S., Caruso G. (2017) The aim of this review is a wide description of the relationships between growing conditions and bulb yield and quality of onion (Allium cepa L.), focused particularly on long-day cultivars suitable for storage. Marketable yield decreases according to the reduction of crop length caused by the increase of growth temperature. The nutritive requirements of storage onion are highest during the vegetative growth. The application of humic substances and the inoculation of mycorrhizae may enhance bulb growth and quality, mainly under stress conditions. Onion is a slow-growth, shallowrooted crop with non-shading habitus and therefore its productivity is highly dependent on water availability in the soil, proper fertilization and weed control. The shelf-life of onion bulbs is a genetic trait, improvable by efficient crop and post-harvest management, and adequate conditions of bulb storage. The quality of storage onion bulbs is ascribed to several indicators, such as thiosulfonates, pyruvic acid, soluble solids, sugars, and many other biological compounds. This review is also focused on onion quality as affected by the interactions among genotype, environment, farming practices and post-harvest management.

show abstract

Section: Genotype and Environmentmentioning

confidence: 99%

Interactions among genotype, environment and agronomic practices on production and quality of storage onion (Allium cepa L.) - A review

Sękara¹,

Pokluda²,

Vacchio³

et al. 2017

Hortic. Sci.

View full text Add to dashboard Cite

show abstract

“…SSR markers were selected to be evenly distributed across each linkage group of the bunching onion map (1-4 loci per linkage group) (Tsukazaki et al 2008). Of these, ACM096, ACE010 and ACE044 are bulb onion ESTderived SSR markers (Kuhl et al 2004, Tsukazaki et al 2008, and AMS14 is a bulb onion genomic SSR marker developed by Fischer and Bachmann (2000).…”

Section: Ssr Markers and Pcrmentioning

confidence: 99%

“…Of these, ACM096, ACE010 and ACE044 are bulb onion ESTderived SSR markers (Kuhl et al 2004, Tsukazaki et al 2008, and AMS14 is a bulb onion genomic SSR marker developed by Fischer and Bachmann (2000). All markers, except for AFS103, have been located on the bunching onion linkage map (Tsukazaki et al 2008).…”

Section: Ssr Markers and Pcrmentioning

confidence: 99%

“…From large-scale sequencing of bulb onion expressed sequence tags (ESTs), hundreds of EST-derived SSR markers have also been developed (Kuhl et al 2004. In our previous study, we isolated thousands of SSRs from a genomic library of bunching onion (Wako et al 2002, Song et al 2004, Tsukazaki et al 2007, and some of these were used to construct a linkage map of bunching onion (Ohara et al 2005b, Tsukazaki et al 2008). The SSR markers, which have been mapped on a linkage map, will be useful for assessing the genetic relationships of bunching onion varieties, although no attempt has been made to use them for the variety classification of bunching onion.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Classification and identification of bunching onion (Allium fistulosum) varieties based on SSR markers

et al. 2010

Self Cite

View full text Add to dashboard Cite

We investigated the correspondence between classifications based on simple sequence repeat (SSR) markers and on morphological traits for 30 bunching onion (Allium fistulosum L.) varieties. We also examined applicability of an assignment test for variety identification in bunching onion. Cluster analysis based on the allele frequency data at 29 SSR loci classified most of the varieties except for 'Iwatsuki-2' into the predicted variety groups, 'Kaga', 'Senju' or 'Kujo', which were categorized based on morphological traits. Although 'Iwatsuki-2' has been regarded as a member of 'Kaga' group, molecular data suggested the variety belonging to 'Kujo' rather than 'Kaga'. In the assignment test at the individual level, 89.1% of the individuals were assigned to their original variety. When the assignment was conducted based on groups each consisting of four individuals, the percentage of correct assignments was considerably improved (99.3%). These results suggested that the assignment test approach will be useful for variety identification in allogamous bunching onions, which have large within-variety genetic diversity. On the other hand, it was also suggested that sampling of true source varieties will be fundamental to avoid misjudgment.

show abstract

“…This QTL is thought to be constantly effective on seedling growth in the present population, showing clear overdominance, as discussed below. Recently, Tsukazaki et al (2008) constructed an SSR-based bunching onion map and assigned the linkage groups to chromosomes of bunching onion and/or bulb onion (Allium cepa L.) using alien monosomic addition lines developed by Shigyo et al (1996). It is suggested that the linkage group D3 corresponds to linkage group 2a on the SSR-based map, which is assigned to A. cepa chromosome 2 (Tsukazaki et al, 2008).…”

Section: Qtls For Seedling Growthmentioning

confidence: 99%

Mapping of Quantitative Trait Loci Controlling Seedling Growth in Bunching Onion (Allium fistulosum L.)

Ohara¹,

Tsukazaki²,

Song³

et al. 2009

J. Japan. Soc. Hort. Sci.

Self Cite

View full text Add to dashboard Cite

Vigorous seedling growth is a desirable trait for machine-assisted transplanting using a plug nursery system in bunching onion. This study was undertaken to identify the quantitative trait loci (QTLs) controlling seedling growth and to elucidate the modes of gene action of the QTLs. Plant material was a set of reciprocally backcrossed populations (BC 1 ). These were produced from 2 inbred lines derived from the Senju group and Kujo group. Two corresponding BC 1 maps were constructed by adding co-dominant simple sequence repeat (SSR) markers to the previously reported amplified fragment length polymorphism (AFLP) based maps. Fresh weights of seedlings were measured in 3 seasons using BC 1 S 1 families. Remarkable heterosis was observed in F 1 and many BC 1 S 1 families. Many QTLs for seedling weight were detected on the 2 maps by composite interval mapping. The phenotypic variation explained by a single QTL was less than 25%. Of these, 3 QTLs on 3 linkage groups are considered to be consistently effective across different environmental conditions because they were detected in different years and seasons. Most QTLs were detected on one map, but were not detected in a corresponding region on the other map; however, the degree of dominance at each locus was calculated to determine whether the locus shows overdominance. Overdominance was observed at some loci, and the other loci showed an additive and dominant effect. It is suggested that remarkable heterosis for seedling weight in the present population is based on the accumulation of dominant and overdominant gene actions.

show abstract

Construction of SSR-based chromosome map in bunching onion (Allium fistulosum)

Cited by 39 publications

References 24 publications

Interactions among genotype, environment and agronomic practices on production and quality of storage onion (Allium cepa L.) - A review

Interactions among genotype, environment and agronomic practices on production and quality of storage onion (Allium cepa L.) - A review

Classification and identification of bunching onion (Allium fistulosum) varieties based on SSR markers

Mapping of Quantitative Trait Loci Controlling Seedling Growth in Bunching Onion (Allium fistulosum L.)

Contact Info

Product

Resources

About