Construction of spider silk protein small‐caliber tissue engineering vascular grafts based on dynamic culture and its performance evaluation

Lu, Hui; Li, Xiafei; Yang, Tao; Liu, Tian; Du, Pengchong; Jing, Changqin; Chen, Zhigang; Lin, Fang; Zhao, Guoan; Zhao, Liang

doi:10.1002/jbm.a.37447

Cited by 5 publications

(4 citation statements)

References 58 publications

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“…These studies were all single studies with limited sample sizes (3 to 46 animals) or with a lack of adequate control groups and variable patency rates. However, some of these studies reported reasonable patency results compared to those in our study (89%): Qiu et al (common carotid artery (CCA) rats, n = 14, 86% patency) [46], Kuwabara et al (CCA rats, n = 46, 77% patency) [44], Janairo et al (CCA rats, n = 8, 100% patency) [47], Sun et al (spider silk protein grafts with ID 1.2 mm in abdominal aorta rats, n = 15, 75-85% patency) [50], and Wakabayashi et al (polyvinyl alcohol coated polycaprolactone grafts with ID 1 mm, n = 12, 83.3% patency) [51]. Therefore, other biomaterials may also be promising candidates, along with ePU.…”

Section: Discussionmentioning

confidence: 54%

“…To date, no clinical studies have been performed using ePU grafts, and studies with larger sample sizes in different (larger) animal models are needed to further confirm the good in vivo performance of ePU grafts with ID < 1.50 mm. Animal studies thus far have failed to translate into clinical trials due to insufficient in vivo mechanical testing and regulatory issues [19,50].…”

Section: Discussionmentioning

confidence: 99%

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Electrospun Polyurethane Vascular Grafts for Cerebral Revascularization: A Pilot Study on Rats

Vergauwen,

Tubeeckx,

Houben

et al. 2024

BioChem

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The current standard technique for vascular grafting in cerebral revascularization surgery employs the interposition of an autologous blood vessel. Technical complications have necessitated the development of a synthetic alternative, but classical biomaterials are not suited for small caliber vascular grafting due to the resulting neointimal hyperplasia and thrombosis. The electrospinning of polymers is a promising technique for the development of small vascular grafts. The in vivo performance and efficacy of electrospun polyurethane (ePU) grafts with an internal diameter of <1.5 mm have thus far not been evaluated. We developed a novel ePU graft, with a diameter of 1.25 mm, for implantation into the infrarenal aorta of rats. The patency rates of grafts after a 4-month period were equal to those reported in other studies using larger ePU graft diameters and equal or higher than in studies employing other biomaterials. We observed some loss in flow velocity throughout the grafts, which suggests a decreased elasticity of the graft compared to that of the native rat aorta. However, the grafts demonstrated good neo-endothelialization and minimal neointimal hyperplasia. Their porosity promoted cellular infiltration, as observed under tissue slide examination. Our results show that ePU vascular grafts with an internal diameter of <1.5 mm are promising candidates for vascular grafting in cerebral revascularization surgery.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Electrospun Polyurethane Vascular Grafts for Cerebral Revascularization: A Pilot Study on Rats

Vergauwen,

Tubeeckx,

Houben

et al. 2024

BioChem

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“…Shear stress is an important factor for remodeling adhesion, morphology, and function of ECs both in vivo and in vitro [ 72 ]. Compared with static culture, dynamic culture is helpful to improve cell proliferation on vascular grafts, which is more beneficial for extracellular matrix deposition and anti-thrombogenesis, as well as reducing the inflammatory response of vascular grafts [ 73 ]. Furthermore, the pretreatment with shear stress enhances the subsequent retention of ECs after implantation in vivo [ 74 ].…”

Section: Resultsmentioning

confidence: 99%

Bilayer vascular grafts with on-demand NO and H2S release capabilities

Liang

Wang

et al. 2024

Bioactive Materials

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“…Taken together, there is increasing interest in engineering SMCs in vascular grafts, using different types of natural-derived (silk protein) or synthetic scaffolds (polycaprolactone). In several studies, which differ in multiple parameters, such as cell origin, culture reagents, and scaffold design, mechanical stimuli seem to be a critical feature towards an SMC phenotype [ 71 , 89 , 90 ].…”

Section: Strategies For Advancing Smc and Skmc Manufacturingmentioning

confidence: 99%

Bioprocessing Considerations towards the Manufacturing of Therapeutic Skeletal and Smooth Muscle Cells

Franchi-Mendes,

Silva,

Cartaxo

et al. 2023

Bioengineering

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Tissue engineering approaches within the muscle context represent a promising emerging field to address the current therapeutic challenges related with multiple pathological conditions affecting the muscle compartments, either skeletal muscle or smooth muscle, responsible for involuntary and voluntary contraction, respectively. In this review, several features and parameters involved in the bioprocessing of muscle cells are addressed. The cell isolation process is depicted, depending on the type of tissue (smooth or skeletal muscle), followed by the description of the challenges involving the use of adult donor tissue and the strategies to overcome the hurdles of reaching relevant cell numbers towards a clinical application. Specifically, the use of stem/progenitor cells is highlighted as a source for smooth and skeletal muscle cells towards the development of a cellular product able to maintain the target cell’s identity and functionality. Moreover, taking into account the need for a robust and cost-effective bioprocess for cell manufacturing, the combination of muscle cells with biomaterials and the need for scale-up envisioning clinical applications are also approached.

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Construction of spider silk protein small‐caliber tissue engineering vascular grafts based on dynamic culture and its performance evaluation

Cited by 5 publications

References 58 publications

Electrospun Polyurethane Vascular Grafts for Cerebral Revascularization: A Pilot Study on Rats

Electrospun Polyurethane Vascular Grafts for Cerebral Revascularization: A Pilot Study on Rats

Bilayer vascular grafts with on-demand NO and H2S release capabilities

Bioprocessing Considerations towards the Manufacturing of Therapeutic Skeletal and Smooth Muscle Cells

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