Construction of Red Fluorescent Dual Targeting Mechanically Interlocked Molecules for Live Cancer Cell Specific Lysosomal Staining and Multicolor Cellular Imaging

Abstract

We have designed and synthesized red fluorescent mechanically interlocked molecules with dual targeting functionality for live cancer cell specific active targeting followed by selective internalization and imaging of malignant lysosomes along with realtime tracking, 3D, and multicolor cellular imaging applications.

Recent advances in combatting bacterial infections via well-designed metallacycles/metallacages

“…Mechanically interlocked molecules (MIMs) have been one of the focuses in supramolecular chemistry research, and they have inherent topological structures and thus challenge the imagination and skills of researchers. 69,70 Molecular necklaces are one of the members of MIMs, which are usually prepared from three parts: a molecular axis as a chain, a macrocycle as a bead, and a molecular linker that connects them. 71,72 In recent years, the well-optimized synthetic tactics and delicate structure of molecular necklaces have gained important breakthroughs, better for further design of functionalized molecular necklaces with heterometallic centers and biological applications.…”

Mechanically interlocked luminescent molecules

“…Guha et al [114] designed a [2]rotaxane 57 comprised of a 1, 3, 3-trimethylindoline SQ dye axle tethered with a morpholine component and an anthracene macrocycle bioconjugated with a cancer cell target-specific peptide by click chemistry (Figure 12b). [2]rotaxane 57 is an effective fluorescent probe for live cancer cell lysosomes owing to its intense and narrow red absorption/emission, high FQY, and stability.…”

“…Guha et al [114] . designed a [2]rotaxane 57 comprised of a 1, 3, 3‐trimethylindoline SQ dye axle tethered with a morpholine component and an anthracene macrocycle bio‐conjugated with a cancer cell target‐specific peptide by click chemistry (Figure 12b).…”

Acidic pH‐Triggered Live‐Cell Lysosome Specific Tracking, Ratiometric pH Sensing, and Multicolor Imaging by Visible to NIR Switchable Cy‐7 Dyes

“…Moreover, multicolor imaging in live cells has been accomplished by utilizing the synthesized probe blending with other organelle staining probes with discrete excitation/ emission bands. [23][24][25]…”

“…Fluorescence intensity in two well‐separated wavelengths (visible λ em =478 and NIR λ em =800 nm) with ∼322 nm bathochromic shift that minimizes cross talk and permits auto‐correction, therefore deviations in signal owing to investigational artifacts like dye concentration, laser power and quenching are avoided, providing precise quantification and ratiometric fluorescence imaging of pH inside the live‐cell lysosomes. Moreover, multicolor imaging in live cells has been accomplished by utilizing the synthesized probe blending with other organelle staining probes with discrete excitation/emission bands [23–25] …”