This study aimed to assess the competence levels of biology teachers in San Mariano and Benito Soliven schools during the 2019-2020 academic year. Employing a quantitative research approach, the study utilized a descriptive-comparative method. Data were collected through two main techniques: administering a test covering Biology content for Grades 7 to 10 and conducting a survey on instruction and assessment practices. The quantitative analysis described respondent profiles and competence levels, with comparisons based on selected profile variables. Frequency and percentages were used to analyze profile data, while correct response computations and percentages were employed to assess competence levels in content. Likert scales were utilized to evaluate instructional and assessment competence. Findings indicated that teacher-respondents achieved an average score of 67.22, qualifying as competent in subject content. Instructional competence received an overall mean rating of 2.42, indicating slight competence, while assessment competence garnered a weighted mean of 2.77, signifying competence. Despite overall competence in instruction and assessment, slight deficiencies were noted in content competence. Therefore, the study recommended providing teachers with opportunities for seminars, training, and workshops to update their knowledge and pedagogical approaches in line with current teaching trends. Such initiatives would enhance teacher effectiveness and ensure alignment with evolving educational standards and methodologies.