Construction of Plasmonic Core–Satellite Nanostructures on Substrates Based on DNA-Directed Self-Assembly as a Sensitive and Reproducible Biosensor

Abstract

We report the successful construction of plasmonic core-satellite nanostructured assemblies on two-dimensional substrates, based on a strategy of combining DNA-functionalized plasmonic nanoparticles (NPs) with the specific recognition ability toward target to enable satellite NPs to self-assemble around the core immobilized on substrates. A strongly coupled plasmonic resonance band was observed because of the close proximity between core and satellite NPs, which presented significant red-shift and enhanced extinction with respect to the local surface plasmon resonance (LSPR) band of individual core NPs on the substrate. The functionality of this core-satellite nanostructured assembly as a biosensor was further explored, and the changes in extinction intensity and the peak shift of the plasmonic coupling resonance band arising from the probe-target DNA binding event all proved to be useful criteria for target DNA detection. Moreover, high selectivity down to single-base mismatched DNA was achieved using this strongly coupled plasmonic core-satellite nanostructured assembly on a substrate. Such substrate-based detection was advantageous, and its reusability and high cycle stability were demonstrated after five cycles of disassembly and reassembly. Our work demonstrates the biosensing capacity of this DNA-functionalized plasmonic nanoassembly model system on two-dimensional substrate, which is also applicable to the detection of numerous DNA-recognized biomolecules. Likewise, the presented construction method can be extended to fabricate other compositional core-satellite nanoassemblies.

High‐Performance Biosensing Systems Based on Various Nanomaterials as Signal Transducers

“…Zhang et al detected target DNA by controlling the plasmonic properties of Au NPs using probe DNA‐modified Au NPs . To capture the target DNA, two different types of DNA probes were respectively introduced on large (40 nm) and small Au NPs (17 nm).…”

“…A) Schematic illustration of the construction of target DNA‐mediated self‐assembly of Au NPs, B) difference in plasmonic properties between the core and core‐satellite structures, and SEM images of C) the core particles and D) the DNA‐mediated core‐satellite hybrid structures. Reproduced with permission . Copyright 2015, American Chemical Society.…”

“…[45,46] Zhang et al detected target DNA by controlling the plasmonic properties of Au NPs using probe DNA-modified Au NPs. [47] To capture the target DNA, two different types of DNA probes were respectively introduced on large (40 nm) and small Au NPs (17 nm). In this case, the probes played the roles of a plasmonic core and satellite for achieving plasmonic coupling interactions.…”

“…Reproduced with permission. [47] Copyright 2015, American Chemical Society. E) Mechanism of target DNA-induced dimerization of Au NPs, F) dependence of the plasmonic absorbance spectrum on the target DNA concentration (numbers 1 to 6 represent 0, 1 pM, 10 pM, 100 pM, 1 nM, and 10 nM, respectively), G) sensitivity test and H) dimerized Au NP structure in the presence of 10 nM target DNA.…”

Metabolic mapping with plasmonic nanoparticle assemblies

“…Detection is achieved via a spectral shift or a change in the intensity of the scattered light, arisen from the reconfiguration of multi-nanoparticle assemblies. [21][22][23][24][25][26] Plasmonic nanostructures such as dimers 21,23,24,[27][28][29] and clusters [30][31][32][33] have been developed for monitoring biomolecular interactions, including enzymatic activities 32,33 and binding of nucleic acids; 34,35 however the direct quantification of intracellular biomolecules from individual cells has not been achieved.…”