Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis

Birkelund, S; Stephens, Richard S.

doi:10.1128/jb.174.9.2742-2747.1992

Cited by 53 publications

(26 citation statements)

References 32 publications

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“…Recently physical and genetic maps of C. trachomatis were reported (2). The genome size of C. trachomatis was determined to be 1,045 kb, which showed good agreement to previous estimation by electron microscopy (18,29).…”

Section: Geneticssupporting

confidence: 69%

Chlamydia pecorum—The Fourth Species of Genus Chlamydia—

Fukushi

Hirai

1993

Microbiology and Immunology

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Section: Geneticssupporting

confidence: 69%

Chlamydia pecorum—The Fourth Species of Genus Chlamydia—

Fukushi

Hirai

1993

Microbiology and Immunology

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“…trachomatis strain L2 was propagated in L929 cells in suspension cultures and the DNA purified from EBs as previously described (Birkelund and Stephens 1992).…”

Section: Growth Of C Trachomatis Strains L2 and Uch-1mentioning

confidence: 99%

Chlamydia trachomatis: Genome sequence analysis of lymphogranuloma venereum isolates

Thomson

Holden²,

Carder³

et al. 2007

Genome Res.

197

180

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Chlamydia trachomatis is the most common cause of sexually transmitted infections in the UK, a statistic that is also reflected globally. There are three biovariants of C. trachomatis: trachoma (serotypes A–C) and two sexually transmitted pathovars; serotypes D–K and lyphogranuloma venereum (LGV). Trachoma isolates and the sexually transmitted serotypes D–K are noninvasive, whereas the LGV strains are invasive, causing a disseminating infection of the local draining lymph nodes. Genome sequences are available for single isolates from the trachoma (serotype A) and sexually transmitted (serotype D) biotypes. We sequenced two isolates from the remaining biotype, LGV, a long-term laboratory passaged strain and the recent “epidemic” LGV isolate-causing proctitis. Although the genome of the LGV strain shows no additional genes that could account for the differences in disease outcome, we found evidence of functional gene loss and identified regions of heightened sequence variation that have previously been shown to be important sites for interstrain recombination. We have used new sequencing technologies to show that the recent clinical LGV isolate causing proctitis is unlikely to be a newly emerged strain but is most probably an old strain with relatively new clinical manifestations.

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“…They studied rickettsiae genomes using randomly chosen DNA probes and RFLP analysis to determine genetic similarity on the basis of percent base pair mismatch. The principal disadvantage of these last two approaches is that only a small part of the genome is studied.Pulsed-field gel electrophoresis (PFGE) appears to be the best way to study the entire genome of SFG rickettsiae, since this has already been performed for other microorganisms (1,11,14,17,33,47,52,57). Several species of the family Rickettsiaceae have been studied by PFGE.…”

mentioning

confidence: 99%

Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis

Roux

Raoult

1993

J Bacteriol

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Using pulsed-field gel electrophoresis, we studied the chromosomes of spotted fever group rickettsiae. We digested the DNA of 16 species currently known to belong to this group with SmaI, EagI, and BssHII. The genome size of 13 rickettsiae was between 1,200 and 1,300 kb. "Ricketisia massiliae" and "R. helvetica" genome sizes were 1,370 and 1,397 kb, respectively, and that ofR. bellii was 1,660 kb. It was possible to obtain distinctive patterns for each species, but in R. conorii, 10 isolates exhibited the same profiles, showing that pulsed-field gel electrophoresis is a good interspecies identification tool. We achieved a phylogenetic analysis of these bacteria by using the Dice coefficient and UPGMA and Package Philip programming. We established a dendrogram of the genetic relationships between the different species showing the existence of a cluster in the spotted fever group rickettsiae including R. conorii, R. rickettsii, R. parkeri, R. sibirica, "R. africae," "R.slovaca," Thai tick typhus rickettsia, and Israeli tick typhus rickettsia. We located three genes previously cloned and sequenced (genes encoding the R. rickettsii surface proteins of 120 and 190 kDa and the R. prowazekii citrate synthase gene), using Southern hybridization. The genes encoding citrate synthase and the surface protein of 190 kDa were usually located on the same band, and it is hypothesized that they are relatively close on the chromosome.The family Rickettsiaceae comprises several genera including the genus Rickettsia, which is subdivided into three groups: typhus, scrub typhus, and spotted fever group (SFG) rickettsiae. SFG rickettsiae have been grouped taxonomically on the basis of morphological, ecological, and antigenic criteria. These bacilli are gram-negative short rods, 0.3 to 0.5 pum in diameter and 0.8 to 2.0 pum in length (sometimes longer when cell division is impaired), which retain basic fuchsin when stained by the method of Gimenez (28) and which grow both in the nucleus and in the cytoplasm of the host cells (60). They are transmitted to humans by infectedarthropod bites or feces.The usual identification methods used in bacteriology are not applicable for rickettsiae because of their strictly intracellular position. The actual classification of SFG rickettsiae is exclusively based on mouse serotyping (41). The antigenic determinant of this serotyping is constituted by two major envelope proteins of high molecular weight called rOmp A and rOmp B (26). In fact, this method allows the identification of new isolates, but no information on the relationships between the different strains is given. It is necessary to define strict criteria of classification, because with the development of a new cell culture isolation technique (the shell vial technique) (36) over the past few years, there has been detection of new isolates everywhere in the world, both from ticks ("Rickettsia massiliae" in the south of France [6,7], MC6 in Morocco [37], GS in Greece [5], and "R. helvetica" in Switzerland [15]) and from humans ("R africae" in...

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Construction of physical and genetic maps of Chlamydia trachomatis serovar L2 by pulsed-field gel electrophoresis

Cited by 53 publications

References 32 publications

Chlamydia pecorum—The Fourth Species of Genus Chlamydia—

Chlamydia pecorum—The Fourth Species of Genus Chlamydia—

Chlamydia trachomatis: Genome sequence analysis of lymphogranuloma venereum isolates

Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis

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