Construction of Microfluidic Chip Structure for Cell Migration Studies in Bioactive Ceramics

Abstract

Cell migration is an essential bioactive ceramics property and critical for bone induction, clinical application, and mechanism research. Standardized cell migration detection methods have many limitations, including a lack of dynamic fluid circulation and the inability to simulate cell behavior in vivo. Microfluidic chip technology, which mimics the human microenvironment and provides controlled dynamic fluid cycling, has the potential to solve these questions and generate reliable models of cell migration in vitro. In this study, a microfluidic chip is reconstructed to integrate the bioactive ceramic into the microfluidic chip structure to constitute a ceramic microbridge microfluidic chip system. Migration differences in the chip system are measured. By combining conventional detection methods with new biotechnology to analyze the causes of cell migration differences, it is found that the concentration gradients of ions and proteins adsorbed on the microbridge materials are directly related to the occurrence of cell migration behavior, which is consistent with previous reports and demonstrates the effectiveness of the microfluidic chip model. This model provides in vivo environment simulation and controllability of input and output conditions superior to standardized cell migration detection methods. The microfluidic chip system provides a new approach to studying and evaluating bioactive ceramics.

Neuroinflammatory response on a newly combinatorial cell–cell interaction chip

“…By establishing cell migration trajectories, qualitative and quantitative characterization of dynamic cell features can be determined, such as migration distance, speed, direction, and duration. 30,31 However, traditional experimental tools cannot simultaneously create an in vitro coculture environment, enable real-time observation, or perform multi-index analysis of cell behavior.…”

Biointerfaces with ultrathin patterns for directional control of cell migration

“…These microfluidic devices offer an effective method for high-throughput screening of drugs. Furthermore, bioactive ceramic microfluidic chips were employed to examine cell migration across various ceramic microbridges [ 31 ]. These chips enable the visual and dynamic observation of migration differences among cells in distinct ceramic microbridges.…”

Microfluidic single-cell migration chip reveals insights into the impact of extracellular matrices on cell movement

“…To address this issue, modern microfabrication technologies have been employed in a number of studies to investigate the movement of individual cells, using microfluidics. 37–58 Recently, we have developed a high-throughput microfluidic migration platform that utilizes robotic liquid handling and computer vision to rapidly quantify individual cellular motility. 59…”