Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice

Chang, Eric H.; Frattini, Stephen A.; Robbiati, Sergio; Huerta, Patricio T.

doi:10.3791/50470

Cited by 22 publications

(24 citation statements)

References 19 publications

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“…Pre-injection recordings (pre) were conducted prior to daily drug administration, and post-injection (post) recordings were conducted approximately 10 min after the injection. During each recording session, we recorded wideband electrical activity (1-1894 Hz, sampling rate of 3787 Hz) using a Neuralynx (Bozeman, MT) data acquisition system (Chang et al, 2013). While the rats were completing the behavioral task, a camera located above the maze tracked each animal movement through monitoring light-emitting diodes attached to the animal's head stage.…”

Section: Behavioral Data Collection and Drug Administrationmentioning

confidence: 99%

Effects of chronic ketamine on hippocampal cross‐frequency coupling: implications for schizophrenia pathophysiology

Michaels

Long

Stevenson

et al. 2018

Eur J of Neuroscience

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Disrupted neuronal oscillations have been identified as a potentially important biomarker for the perceptual and cognitive symptoms of schizophrenia. Emerging evidences suggest that interactions between different frequency bands, cross-frequency coupling (CFC), serve an important role in integrating sensory and cognitive information and may contribute to disease pathophysiology. In this study, we investigated the effects of 14-day consecutive administration of ketamine (30 mg/kg i.p.) vs. saline on alterations in amplitude and changes in the coupling of low-frequency (0-30 Hz) phase and high-frequency (30-115 Hz) amplitude in the CA1 hippocampus of Long Evans rats. Intracranial electrode recordings were conducted pre- and post-injection while the animals performed a foraging task on a four-arm rectangular maze. Permutation analysis of frequency band-specific change in amplitudes revealed between-group differences in theta (6-12 Hz) and slow gamma (25-50 Hz) but not fast gamma (65-100 Hz) bands at both slow and fast speeds. Chronic ketamine challenge resulted in decreased coupling (pre to post) at slow speeds but increased coupling at faster speeds, compared to either no or modest increased coupling in the saline group. These results demonstrate that chronic ketamine administration alters the interaction of low-frequency phase and high-frequency oscillations chronically and that such coupling varies as a function of locomotive speed. These findings provide evidence for the potential relevance of CFC to the pathophysiology of schizophrenia.

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Section: Behavioral Data Collection and Drug Administrationmentioning

confidence: 99%

Effects of chronic ketamine on hippocampal cross‐frequency coupling: implications for schizophrenia pathophysiology

Michaels

Long

Stevenson

et al. 2018

Eur J of Neuroscience

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“…Two bipolar stimulating electrodes were implanted unilaterally, targeting the left medial forebrain bundle (MFB; 1.9mm & 1.4mm posterior to bregma, 0.8 mm lateral, 4.8 mm below dura). A 16-tetrode recording array (Chang et al, 2013) was implanted, targeting PC and hippocampus (2.2mm posterior to bregma, 2.0mm lateral). Animals recovered for one week, during which tetrodes targeting PC were moved down 31m daily for the first three days, and then every other day, to prevent sticking.…”

Section: Methods Detailsmentioning

confidence: 99%

“…Procedures. An electrode interface board (EIB-72-QC-Small, EIB-36-16TT Neuralynx) was attached with a custom adapter to the recording array (Chang et al, 2013) with independently drivable tetrodes connected via a pair of unity-gain headstages (HS-36 Neuralynx) to the recording system (Digital Lynx SX Neuralynx). Tetrodes were referenced to a tetrode wire in the corpus callosum and advanced as needed, up to 62m/day, while monitoring the audio and visual signal of the unit activity.…”

Section: Recordingmentioning

confidence: 99%

Impaired Hippocampal-cortical interactions during sleep and memory reactivation without consolidation in a mouse model of Alzheimer’s disease

Benthem¹,

Skelin

Moseley³

et al. 2019

Preprint

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Spatial learning is impaired in preclinical Alzheimer's disease (AD). We reported similar impairments in 3xTg-AD mice learning a spatial reorientation task. Memory reactivation during sleep is critical for learning related plasticity, and memory consolidation is correlated with hippocampal sharp wave ripple (SWR) density, cortical delta waves (DWs), and their temporal coupling -postulated as a physiological substrate of memory consolidation. Finally, hippocampal-cortical dyscoordination is prevalent in individuals with AD. Thus, we hypothesized impaired memory consolidation mechanisms in hippocampal-cortical networks could account for spatial memory deficits. We assessed sleep architecture, SWR/DW dynamics and memory reactivation in a mouse model of tauopathy and amyloidosis implanted with a recording array targeting isocortex and hippocampus. Mice underwent daily recording sessions of rest-task-rest while learning the spatial reorientation task. We assessed memory reactivation by matching activity patterns from the approach to the unmarked reward zone to patterns during slow wave sleep (SWS). AD mice had more SWS, but reduced SWR density. The increased SWS compensated for reduced SWR density so there was no reduction in SWR number. Conversely, DW density was not reduced so the number of DWs was increased. In control mice hippocampal SWR-cortical DW coupling was strengthened in post-tasksleep and was correlated with performance on the spatial reorientation task the following day. However, in AD mice SWR-DW coupling was reduced and not correlated with behavior, suggesting behavioral decoupling. Thus, reduced SWR-DW coupling may cause impaired learning in AD and may serve as a biomarker for early AD related changes. Significance StatementUnderstanding the relationship between network dynamics and cognition early in Alzheimer's disease progression is critical for identifying therapeutic targets for earlier treatment. We assessed hippocampal-cortical interactions during sleep in AD mice as a potential cause of early spatial learning and memory deficits. We identified compensatory sleep changes in AD mice, that ameliorated some brain dysfunction. Despite the compensatory changes, impaired spatial navigation and impaired hippocampal-cortical (sharp wave ripple-delta wave) interactions were apparent in AD mice. In control but not AD mice hippocampal-cortical interactions were correlated with performance on the spatial task, the following day, suggesting a potential mechanism of impaired consolidation in AD mice. Thus, changes in hippocampal-cortical brain dynamics during sleep may underlie early memory deficits in AD.

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“…More recent work has made significant strides in weight reduction while at the same time allowing for increased flexibility and higher channel counts [11–13]. There are now a wide variety of design approaches.…”

Section: Introductionmentioning

confidence: 99%

OptoZIF Drive: a 3D printed implant and assembly tool package for neural recording and optical stimulation in freely moving mice

et al. 2016

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Objective Behavioral neuroscience studies in freely moving rodents require small, light-weight implants to facilitate neural recording and stimulation. Our goal was to develop an integrated package of 3D printed parts and assembly aids for labs to rapidly fabricate, with minimal training, an implant that combines individually positionable microelectrodes, an optical fiber, zero insertion force (ZIF-clip) headstage connection, and secondary recording electrodes, e.g. for electromyograms (EMG). Approach Starting from previous implant designs that position recording electrodes using a control screw, we developed an implant where the main drive body, protective shell, and non-metal components of the microdrives are 3D printed in parallel. We compared alternative shapes and orientations of circuit boards for electrode connection to the headstage, in terms of their size, weight, and ease of wire insertion. We iteratively refined assembly methods, and integrated additional assembly aids into the 3D printed casing. Main Results We demonstrate the effectiveness of the OptoZIF Drive by performing real time optogenetic feedback in behaving mice. A novel feature of the OptoZIF Drive is its vertical circuit board, which facilities direct ZIF-clip connection. This feature requires angled insertion of an optical fiber that still can exit the drive from the center of a ring of recording electrodes. We designed an innovative 2-part protective shell that can be installed during the implant surgery to facilitate making additional connections to the circuit board. We use this feature to show that facial EMG in mice can be used as a control signal to lock stimulation to the animal's motion, with stable EMG signal over several months. To decrease assembly time, reduce assembly errors, and improve repeatability, we fabricate assembly aids including a drive holder, a drill guide, an implant fixture for microelectode “pinning”, and a gold plating fixture. Significance The expanding capability of optogenetic tools motivates continuing development of small optoelectric devices for stimulation and recording in freely moving mice. The OptoZIF Drive is the first to natively support ZIF-clip connection to recording hardware, which further supports a decrease in implant cross-section. The integrated 3D printed package of drive components and assembly tools facilities implant construction. The easy interfacing and installation of auxiliary electrodes makes the OptoZIF Drive especially attractive for real time feedback stimulation experiments.

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Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice

Cited by 22 publications

References 19 publications

Effects of chronic ketamine on hippocampal cross‐frequency coupling: implications for schizophrenia pathophysiology

Effects of chronic ketamine on hippocampal cross‐frequency coupling: implications for schizophrenia pathophysiology

Impaired Hippocampal-cortical interactions during sleep and memory reactivation without consolidation in a mouse model of Alzheimer’s disease

OptoZIF Drive: a 3D printed implant and assembly tool package for neural recording and optical stimulation in freely moving mice

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