Construction of luminescent Escherichia coli via expressing lux operons and their application on toxicity test

Yang, Jingfeng; Hu, Shulin; Wu, Maochun; Liao, Anqi; Liang, Shuli; Lin, Ying

doi:10.1007/s00253-022-12136-1

Cited by 2 publications

(1 citation statement)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Photobacterium phosphoreum is also a marine bacterium, and it needs NaCl (>3%) to glow (Table 1). Therefore, it is also affected by Na + and Cl − during detection [67].…”

Section: Photobacterium Phosphoreum In the Detection Of Pollutants In...mentioning

confidence: 99%

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

et al. 2023

View full text Add to dashboard Cite

The luminescent bacteria bioassay has been commonly used in the detection of environmental pollutants. Compared with traditional chemical and other biological detection methods, the luminescent bacteria bioassay has many demonstrated advantages such as a sensitive response, low cost, high efficiency, and environmental friendliness. The traditional luminescent bacteria bioassay has poor reproducibility and cannot achieve undisturbed soil testing, and the use of leach liquor also affects the results. This paper reviews the research progress and existing issues for the traditional luminescent bacteria bioassay used in the detection of soil pollutants. The luminescence mechanisms and detection principles of three commonly used luminescent bacteria, i.e., Vibrio fischeri, Photobacterium phosphoreum, and Vibrio qinghaiensis, are discussed and compared. In addition, two new luminescent bacteria bioassays are introduced to detect soil pollutants. One method is based on recombinant luminescent bacteria obtained with a gene-modification technique. This method can realize specific detection and enhance sensitivity, but it still cannot achieve undisturbed soil detection. The other method involves using magnetic nanoparticle (MNP)-based biosensors made from luminescent bacteria and MNPs. It can realize the accurate detection of the biological toxicity of the combined pollutants in soil without disturbing the soil’s integrity. This study shows that MNP-based biosensors have good application prospects in soil pollution detection, but the mechanism behind their utility still needs to be investigated to realize their popularization and application.

show abstract

“…Photobacterium phosphoreum is also a marine bacterium, and it needs NaCl (>3%) to glow (Table 1). Therefore, it is also affected by Na + and Cl − during detection [67].…”

Section: Photobacterium Phosphoreum In the Detection Of Pollutants In...mentioning

confidence: 99%

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

et al. 2023

View full text Add to dashboard Cite

show abstract

Insight into the mechanisms of combined toxicity of cadmium and flotation agents in luminescent bacteria: Role of micro/nano particles

Sun,

Yan,

Chen

et al. 2024

Science of The Total Environment

View full text Add to dashboard Cite

Construction of luminescent Escherichia coli via expressing lux operons and their application on toxicity test

Cited by 2 publications

References 45 publications

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

Insight into the mechanisms of combined toxicity of cadmium and flotation agents in luminescent bacteria: Role of micro/nano particles

Contact Info

Product

Resources

About