Construction of lncRNA TYMSOS/hsa-miR-101-3p/CEP55 and TYMSOS/hsa-miR-195-5p/CHEK1 Axis in Non-small Cell Lung Cancer

Ji, Longtao; Yang, Ting; Liu, Man; Li, Jiaqi; Si, Qiufang; Wang, Yulin; Liu, Jingjing; Dai, Liping

doi:10.1007/s10528-022-10299-0

Cited by 5 publications

(8 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As an example, TYMSOS enhanced the progression of LUAD. [26][27][28] GSEC promoted proliferation, migration and invasion in LUAD cells. [29][30][31] Potential regulatory pathways in LUAD, TYMSOS and GSEC/hsa-miR-101-3p/IQGAP3 were identified.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Identification and validation of non‐coding RNA‐mediated high expression of IQGAP3 in poor prognosis of lung adenocarcinoma

Su,

Wang,

Cao

et al. 2024

The Journal of Gene Medicine

View full text Add to dashboard Cite

BackgroundThe primary reason for tumor‐related deaths worldwide is lung adenocarcinoma (LUAD). The oncogene IQ motif‐containing GTPase activating protein 3 (IQGAP3) is crucial for contributing to tumor initiation and progression. However, the precise function and molecular mechanism of IQGAP3 in LUAD remain unknown. The present study aimed to investigate the expression, prognosis, mechanism and tumor immunity associated with IQGAP3 in LUAD.MethodsThe relationship between IQGAP3 and the poor prognosis of LUAD was analyzed using The Cancer Genome Atlas (TCGA) database. This analysis was further validated on lung cancer tissues and cell lines. The function of IQGAP3 was investigated by silencing it in LUAD cell lines. To predict microRNA (miRNA) and long non‐coding RNA associated with IQGAP3, the starBase database was utilized, and the predictions were verified by enhancing the function of miRNA. Finally, the relationship between IQGAP3 and tumor immunity was evaluated using Spearman's correlation analysis.ResultsTCGA database revealed that higher levels of IQGAP3 were associated with advanced tumor stage, N stage and poor prognosis in LUAD patients. To confirm that, we conducted experiments on lung cancer tissues and cell lines and found that silencing IQGAP3 significantly inhibited tumor cell proliferation and migration. The expression of IQGAP3 showed a negative correlation with has‐miR‐101‐3p and has‐miR‐135a‐5p, whereas it showed a positive correlation with GSEC, AC005034.3 and TYMSOS. Furthermore, the introduction of miRNA‐mimics into lung cancer cell resulted in a significant inhibition of cancer cell growth and migration. Following that, the level of IQGAP3 showed a positive correlation with the infiltration of immune cells in tumors.ConclusionsThese results reveal that IQGAP3 significantly promotes LUAD progression and could serve as a prognostic biomarker for LUAD. Furthermore, IQGAP3 is most likely regulated by the GSEC/TYMSOS‐hsa‐miR‐101‐3p axis and the AC005034.3‐hsa‐miR‐135a‐5p axis in LUAD.

show abstract

Section: Discussionmentioning

confidence: 89%

“…Several malignancies are associated with the two lncRNAs TYMSOS and GSEC. As an example, TYMSOS enhanced the progression of LUAD 26–28 . GSEC promoted proliferation, migration and invasion in LUAD cells 29–31 .…”

Section: Discussionmentioning

confidence: 93%

Identification and validation of non‐coding RNA‐mediated high expression of IQGAP3 in poor prognosis of lung adenocarcinoma

Su,

Wang,

Cao

et al. 2024

The Journal of Gene Medicine

View full text Add to dashboard Cite

show abstract

“…In the past decade, emerging evidence illustrates that TYMSOS functions as an oncogene in different cancers, including GC, NSCLC, thyroid carcinoma, and osteosarcoma. 6 , 7 , 8 , 9 , 10 Several TYMSOS-associated competing endogenous networks have been identified. For instance, FOXM1/TYMSOS/miR-214-3p/NCAPG axis facilitates NSCLC progression by modulating cell proliferation, stemness, migration, and immune cell infiltration.…”

Section: Discussionmentioning

confidence: 99%

“… 5 Aberrant expression of thymidylate synthetase opposite strand (TYMSOS) has been observed in different cancers. 6 , 7 , 8 , 9 , 10 For instance, TYMSOS promotes cell proliferation, migration, and invasion in gastric cancer (GC) through miR-4739/ZNF703 axis. 6 It is also identified as an oncogene in non-small-cell lung cancer (NSCLC), thyroid carcinoma, and osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

LncRNA TYMSOS facilitates breast cancer metastasis and immune escape through downregulating ULBP3

Zhang¹,

Tan²,

Guo³

2023

iScience

View full text Add to dashboard Cite

High expression of PAFAH1B3 results in poor prognosis in lung adenocarcinoma patients and is associated with tumor cell pyroptosis genes

Hu,

Du,

Yuan

et al. 2024

CCB

View full text Add to dashboard Cite

Background Lung adenocarcinoma (LUAD) is a common cancer with a poor prognosis. Platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29 kDa (PAFAH1B3) plays an important role in the development of many types of human malignancies. However, the precise role and mechanisms of PAFAH1B3 in LUAD are still unknown.Therefore, we will initially explore the effect of PAFAH1B3 on LUAD in this study. Methods In this study, we first performed a pan-cancer analysis of PAFAH1B3 expression and prognosis using The Cancer Genome Atlas (TCGA), genotype-tissue expression (GTEx) data, and GEPIA database. Next, the relationship between PAFAH1B3 expression and LUAD immune infiltration and pyroptosis-related genes was explored by GEPIA database and TIMER database. The effect of PAFAH1B3 on LUAD was further explored by CCK-8, wound healing, and Transwell assays. Finally, non-coding RNA (ncRNA) that may be involved in the regulation of PAFAH1B3 was explored using Starbase database analysis. Results The results found that PAFAH1B3 may be an oncogene in LUAD and has a significant adverse relationship with tumor immune cell infiltration, immune cell biomarkers and pyroptosis-related gene expression. Meanwhile, cell experiments also found that PAFAH1B3 knockout significantly reduced the proliferation, migration and invasion of A549 cells. Conclusions PAFAH1B3 high expression in LUAD patients is associated with poor prognosis, tumor immune infiltration, and cell pyroptosis gene expression.

show abstract

Construction of lncRNA TYMSOS/hsa-miR-101-3p/CEP55 and TYMSOS/hsa-miR-195-5p/CHEK1 Axis in Non-small Cell Lung Cancer

Cited by 5 publications

References 61 publications

Identification and validation of non‐coding RNA‐mediated high expression of IQGAP3 in poor prognosis of lung adenocarcinoma

Identification and validation of non‐coding RNA‐mediated high expression of IQGAP3 in poor prognosis of lung adenocarcinoma

LncRNA TYMSOS facilitates breast cancer metastasis and immune escape through downregulating ULBP3

High expression of PAFAH1B3 results in poor prognosis in lung adenocarcinoma patients and is associated with tumor cell pyroptosis genes

Contact Info

Product

Resources

About