Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers

Abstract

Lentil (Lens culinaris ssp. culinaris), is a self-pollinating diploid (2n = 2x = 14), cool-season legume crop and is consumed worldwide as a rich source of protein (~24.0%), largely in vegetarian diets. Here we report development of a genetic linkage map of Lens using 114 F(2) plants derived from the intersubspecific cross between L 830 and ILWL 77. RAPD (random amplified polymorphic DNA) primers revealed more polymorphism than ISSR (intersimple sequence repeat) and SSR (simple sequence repeat) markers. The highest proportion (30.72%) of segregation distortion was observed in RAPD markers. Of the 235 markers (34 SSR, 9 ISSR and 192 RAPD) used in the mapping study, 199 (28 SSRs, 9 ISSRs and 162 RAPDs) were mapped into 11 linkage groups (LGs), varying between 17.3 and 433.8 cM and covering 3843.4 cM, with an average marker spacing of 19.3 cM. Linkage analysis revealed nine major groups with 15 or more markers each and two small LGs with two markers each, and 36 unlinked markers. The study reported assigning of 11 new SSRs on the linkage map. Of the 66 markers with aberrant segregation, 14 were unlinked and the remaining 52 were mapped. ISSR and RAPD markers were found to be useful in map construction and saturation. The current map represents maximum coverage of lentil genome and could be used for identification of QTL regions linked to agronomic traits, and for marker-assisted selection in lentil.

Diversification of indigenous gene- pool by using exotic germplasm in lentil (Lens culinaris Medikus subsp. culinaris)

“…In another study, transferrable SSR markers were also highly polymorphic in lentil (Datta et al 2011). However, in contrast to this, Gupta et al (2012) identified only 4.43 % SSR markers polymorphic. It was because they screened SSR markers only in two genotypes and used agrose gel for separation of PCR amplified product.…”

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…The DNA-based markers, such as RFLP (Havey and Muehlbauer, 1989;Muench et al, 1991;Rajora and Mahon, 1994), RAPD (Sharma et al, 1995;Ferguson et al, 2000;Sonnante and Pignone, 2001;Toklu et al, 2009;Tewari et al, 2012), AFLP (Sharma et al, 1996;Zavodna et al, 2000;Duran and Perez de le Vega, 2004;Kahraman et al, 2004;Rubeena et al, 2006;Fiocchetti et al, 2009;Toklu et al, 2009), microsatellite markers (Duran and Perez de le Hamwieh et al, 2009;Liu et al, 2008;Babayeva et al, 2009;Reddy et al, 2010;Gupta et al, 2012, Tewari et al, 2012Zaccardelli et al, 2012), inter simple sequence repeat (ISSR) (Zavodna et al, 2000;Sonnante and Pignone, 2001;Toklu et al, 2009), internal transcribed spacers (ITS) (Fernandez et al, 2000;Mayer and Bagga, 2002;Sonnante et al, 2003), non-transcribed spacer (NTS) (Fernandez et al, 2005), sequenced tagged microsatellite site (STMS) (Inder et al, 2008;Datta et al, 2011), single-nucleotide polymorphisms (SNPs) (Alo et al, 2011), and resistance gene analogue (RGA) (Yaish et al, 2004;Sari et al, 2013) were used to analyze phylogenetic relationships among taxa in the genus Lens, as well as the genetic diversity of cultivated lentil.…”

Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb

“…DNA markers are effective tools in evaluating genetic diversity (Fernandez et al, 2002;Phong et al, 2011), constructing genetic maps (Gupta et al, 2012), and molecular identification and classification (Fernandez et al, 2002). Random amplified polymorphic DNA (RAPD) (Williams et al, 1990) and inter-simple sequence repeat (ISSR) (Zietkiewicz et al, 1994) markers are polymerase chain reaction (PCR)-amplified approaches to acquire the genetic information of populations or individuals.…”