Construction of histamine aptamer sensor based on Au NPs nanozyme for ultrasensitive SERS detection of histamine

Jiang, Huan; Tang, Ruyi; Tan, Yaoyu; Xia, Xuemin; Zhang, Xia

doi:10.1016/j.jfca.2023.105337

Cited by 9 publications

(3 citation statements)

References 39 publications

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“…Additionally, surface-enhanced Raman spectroscopy (SERS) [20][21][22], and ELISA [23,24], have also been intensely researched as alternative routes for histamine detection. These detection methods are based on the activity of enzymes and nanoenzymes, and their bioconjugation of histamine to produce an optoelectronic signal [25]. However, optoelectronic transducers exhibit a few drawbacks, such as the requirement of special equipment, possible influence from natural radiation, whose removal would involve a compensation method, and the response being delayed because of the mass transfer.…”

Section: Introductionmentioning

confidence: 99%

Spectro-Electrochemical Properties of A New Non-Enzymatic Modified Working Electrode Used for Histamine Assessment in the Diagnosis of Food Poisoning

Iordache,

Zubarev

et al. 2023

Foods

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We successfully prepared a non-enzymatic sensor based on a graphene-thiophene composite for histamine detection. The self-assembling properties of the thiophene onto Au support and the high electrical conductivity of graphene encouraged the choice of this type of composite. The composite was deposited via electrochemical polymerization onto the Au layer of a screen-printed microelectrode. The electropolymerization and electrochemical detection of histamine were both achieved by cyclic voltammetry. Two types of electrolytes were used for the electrochemical detection: (a) phosphate buffer solution (PBS), which showed low-intensity redox peaks for histamine; and (b) trichloroacetic acid (TCA) 0.01 M, which showed improved results over PBS and did not damage the microelectrode. For the concentration range of 100–200 mg/kg, the sensor shows a linear regression pattern for the oxidation peak fitted on the equation Ipa = 123.412 + 0.49933 ×x, with R2 = 0.94178. The lowest limit of detection was calculated to be 13.8 mg/kg and the limit of quantification was calculated at 46 mg/kg. These results are important since by monitoring the amount of histamine in a food product, early onset of spoilage can be easily detected, thus reducing foodborne poisoning and food waste (by recycling products that are still edible).

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Section: Introductionmentioning

confidence: 99%

Spectro-Electrochemical Properties of A New Non-Enzymatic Modified Working Electrode Used for Histamine Assessment in the Diagnosis of Food Poisoning

Iordache,

Zubarev

et al. 2023

Foods

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“…24−26 Thus, with similar binding affinities, aptamers can replace the role of antibodies. 27 The aptamers with specificity and chemical stability were selected by systematically evaluating ligands using the exponential enrichment (SELEX) technique through complementary binding of the DNA and targets. To date, various aptamers have been developed to target exosomes.…”

mentioning

confidence: 99%

“…The development of aptamers has opened a new path for bioprobe construction owing to their lower cost, faster production time, and easy modification than antibodies. In addition, these DNAs can bind toxic substances undetectable by antibodies. − Thus, with similar binding affinities, aptamers can replace the role of antibodies . The aptamers with specificity and chemical stability were selected by systematically evaluating ligands using the exponential enrichment (SELEX) technique through complementary binding of the DNA and targets.…”

mentioning

confidence: 99%

Extended-Gate Field-Effect Transistor Consisted of a CD9 Aptamer and MXene for Exosome Detection in Human Serum

Park

Kim

et al. 2023

ACS Sens.

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Cancer progresses silently to the terminal stage of the impossible operable condition. There are many limitations in the treatment options of cancer, but diagnosis in an early stage can improve survival rates and low recurrence. Exosomes are the biomolecules released from cancer cells and are promising candidates for clinical diagnosis. Among them, the cluster of differentiation 9 (CD9) protein is an important exosomal biomarker that can be used for exosome determination. Therefore, here, a CD9 aptamer was first synthesized and applied to an extended-gate field-effect transistor (EGFET)-type biosensor containing a disposable sensing membrane to suggest the possibility of detecting exosomes in a clinical environment. Systematically evaluating ligands using the exponential enrichment (SELEX) technique was performed to select nucleic acid sequences that can specifically target the CD9 protein. Exosomes were detected according to the electrical signal changes on a membrane, which is an extended gate using an Au microelectrode. The fabricated biosensor showed a limit of detection (LOD) of 10.64 pM for CD9 proteins, and the detection range was determined from 10 pM to 1 μM in the buffer. In the case of the clinical test, the LOD and detection ranges of exosomes in human serum samples were 6.41 × 102 exosomes/mL and 1 × 103 to 1 × 107 exosomes/mL, respectively, showing highly reliable results with low error rates. These findings suggest that the proposed aptasensor can be a powerful tool for a simple and early diagnosis of exosomes.

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Sensitive colorimetric aptasensor for multiple foodborne pathogens detection based on PCN-Mo peroxidase-like activity

Li,

Yang,

et al. 2024

Food Bioscience

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Construction of histamine aptamer sensor based on Au NPs nanozyme for ultrasensitive SERS detection of histamine

Cited by 9 publications

References 39 publications

Spectro-Electrochemical Properties of A New Non-Enzymatic Modified Working Electrode Used for Histamine Assessment in the Diagnosis of Food Poisoning

Spectro-Electrochemical Properties of A New Non-Enzymatic Modified Working Electrode Used for Histamine Assessment in the Diagnosis of Food Poisoning

Extended-Gate Field-Effect Transistor Consisted of a CD9 Aptamer and MXene for Exosome Detection in Human Serum

Sensitive colorimetric aptasensor for multiple foodborne pathogens detection based on PCN-Mo peroxidase-like activity

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